Alginate/Pluronic F127-based Encapsulation Supports Viability and Functionality of Human Dental Pulp Stem Cell-derived Insulin-producing Cells

Overview

Journal J Biol Eng

Publisher Biomed Central

Specialty Biomedical Engineering

Date 2020 Aug 29

PMID 32855655

Citations 5

Authors

Suryo Kuncorojakti

Watchareewan Rodprasert

Supansa Yodmuang

Thanaphum Osathanon

Prasit Pavasant

Sayamon Srisuwatanasagul

Chenphop Sawangmake

Affiliations

Soon will be listed here.

Abstract

Background: Current approach for diabetes treatment remained several adverse events varied from gastrointestinal to life-threatening symptoms. Regenerative therapy regarding Edmonton protocol has been facing serious limitations involving protocol efficiency and safety. This led to the study for alternative insulin-producing cell (IPC) resource and transplantation platform. In this study, evaluation of encapsulated human dental pulp-derived stem cell (hDPSC)-derived IPCs by alginate (ALG) and pluronic F127-coated alginate (ALGPA) was performed.

Results: The results showed that ALG and ALGPA preserved hDPSC viability and allowed glucose and insulin diffusion in and out. ALG and ALGPA-encapsulated hDPSC-derived IPCs maintained viability for at least 336 h and sustained pancreatic endoderm marker ), pancreatic islet markers ( and ), and intracellular pro-insulin and insulin expressions for at least 14 days. Functional analysis revealed a glucose-responsive C-peptide secretion of ALG- and ALGPA-encapsulated hDPSC-derived IPCs at 14 days post-encapsulation.

Conclusion: ALG and ALGPA encapsulations efficiently preserved the viability and functionality of hDPSC-derived IPCs in vitro and could be the potential transplantation platform for further clinical application.

Citing Articles

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Differentiation of Alginate-Encapsulated Wharton Jelly-Derived Mesenchymal Stem Cells into Insulin Producing Cells.

Poursafavi Z, Abroun S, Kaviani S, Roodbari N Cell J. 2022; 24(8):449-457.

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Purbantoro S, Osathanon T, Nantavisai S, Sawangmake C Heliyon. 2022; 8(7):e09936.

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