Comparison of Glucose, Acetate and Ethanol As Carbon Resource for Production of Poly(3-Hydroxybutyrate) and Other Acetyl-CoA Derivatives

Overview

Journal Front Bioeng Biotechnol

Date 2020 Aug 28

PMID 32850713

Citations 14

Authors

Shenmei Sun

Yamei Ding

Min Liu

Mo Xian

Guang Zhao

Affiliations

Soon will be listed here.

Abstract

Poly(3-hydroxybutyrate) (PHB) is a biodegradable and biocompatible thermoplastic, and synthesized from the central metabolite acetyl-CoA. The acetyl-CoA synthesis from glucose presents low atomic economy due to the release of CO in pyruvate decarboxylation. As ethanol and acetate can be converted into acetyl-CoA directly, they were used as carbon source for PHB production in this study. The reductase mutant AdhE A267T/E568K was introduced into to enable growth on ethanol, and acetate utilization was improved by overexpression of acetyl-CoA synthetase ACS. Comparison of the PHB production using glucose, ethanol or acetate as sole carbon source showed that the production and yield from ethanol was much higher than those from glucose and acetate, and metabolome analysis revealed the differences in metabolism of glucose, ethanol and acetate. Furthermore, other acetyl-CoA derived chemicals including 3-hydroxypropionate and phloroglucinol were produced from those three feedstocks, and similar results were achieved, suggesting that ethanol could be a suitable carbon source for the production of acetyl-CoA derivatives.

Citing Articles

Transcriptome Analysis Revealed the Molecular Mechanism of Acetic Acid Increasing Pigment Production in CICC41233.

Wang Y, Wu W, Wu X, Li W, Cui J, Long C J Fungi (Basel). 2025; 11(1).

PMID: 39852468 PMC: 11767103. DOI: 10.3390/jof11010049.

Lysine acetylation decreases enzyme activity and protein level of lactate dehydrogenase.

Liu M, Huo M, Guo L, Fu Y, Xian M, Qi Q Eng Microbiol. 2024; 2(4):100045.

PMID: 39628700 PMC: 11611030. DOI: 10.1016/j.engmic.2022.100045.

Microbial conversion of ethanol to high-value products: progress and challenges.

Sun M, Gao A, Liu X, Bai Z, Wang P, Ledesma-Amaro R Biotechnol Biofuels Bioprod. 2024; 17(1):115.

PMID: 39160588 PMC: 11334397. DOI: 10.1186/s13068-024-02546-w.

PHB production by Bacillus megaterium strain MNSH1-9K-1 using low-cost media.

Rivas-Castillo A, Valdez-Calderon A, Angeles-Padilla A, Figueroa-Ocampo C, Carrillo-Ibarra S, Quezada-Cruz M Braz J Microbiol. 2024; 55(1):245-254.

PMID: 38212508 PMC: 10920526. DOI: 10.1007/s42770-023-01232-7.

Perspectives for Using CO as a Feedstock for Biomanufacturing of Fuels and Chemicals.

Kurt E, Qin J, Williams A, Zhao Y, Xie D Bioengineering (Basel). 2023; 10(12).

PMID: 38135948 PMC: 10740661. DOI: 10.3390/bioengineering10121357.

References

Enjalbert B, Cocaign-Bousquet M, Portais J, Letisse F . Acetate Exposure Determines the Diauxic Behavior of Escherichia coli during the Glucose-Acetate Transition. J Bacteriol. 2015; 197(19):3173-81. PMC: 4560281. DOI: 10.1128/JB.00128-15. View

Clark D . The fermentation pathways of Escherichia coli. FEMS Microbiol Rev. 1989; 5(3):223-34. DOI: 10.1016/0168-6445(89)90033-8. View

Cao Y, Jiang X, Zhang R, Xian M . Improved phloroglucinol production by metabolically engineered Escherichia coli. Appl Microbiol Biotechnol. 2011; 91(6):1545-52. DOI: 10.1007/s00253-011-3304-5. View

Steinbuchel A, Schlegel H . Physiology and molecular genetics of poly(beta-hydroxy-alkanoic acid) synthesis in Alcaligenes eutrophus. Mol Microbiol. 1991; 5(3):535-42. DOI: 10.1111/j.1365-2958.1991.tb00725.x. View

Hong P, Zhang J, Liu X, Tan T, Li Z . Effect of NADH kinase on poly-3-hydroxybutyrate production by recombinant Escherichia coli. J Biosci Bioeng. 2016; 122(6):685-688. DOI: 10.1016/j.jbiosc.2016.06.005. View

Meng D, Shi Z, Wu L, Zhou Q, Wu Q, Chen J . Production and characterization of poly(3-hydroxypropionate-co-4-hydroxybutyrate) with fully controllable structures by recombinant Escherichia coli containing an engineered pathway. Metab Eng. 2012; 14(4):317-24. DOI: 10.1016/j.ymben.2012.04.003. View

Aldor I, Keasling J . Metabolic engineering of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) composition in recombinant Salmonella enterica serovar typhimurium. Biotechnol Bioeng. 2001; 76(2):108-14. DOI: 10.1002/bit.1150. View

Liu C, Ding Y, Zhang R, Liu H, Xian M, Zhao G . Functional balance between enzymes in malonyl-CoA pathway for 3-hydroxypropionate biosynthesis. Metab Eng. 2016; 34:104-111. DOI: 10.1016/j.ymben.2016.01.001. View

Chen Y, Lin E . Regulation of the adhE gene, which encodes ethanol dehydrogenase in Escherichia coli. J Bacteriol. 1991; 173(24):8009-13. PMC: 212600. DOI: 10.1128/jb.173.24.8009-8013.1991. View

10.

Fic E, Bonarek P, Gorecki A, Kedracka-Krok S, Mikolajczak J, Polit A . cAMP receptor protein from escherichia coli as a model of signal transduction in proteins--a review. J Mol Microbiol Biotechnol. 2008; 17(1):1-11. DOI: 10.1159/000178014. View

11.

Yuzawa S, Kim W, Katz L, Keasling J . Heterologous production of polyketides by modular type I polyketide synthases in Escherichia coli. Curr Opin Biotechnol. 2012; 23(5):727-35. DOI: 10.1016/j.copbio.2011.12.029. View

12.

Brandl H, Gross R, Lenz R, Fuller R . Pseudomonas oleovorans as a Source of Poly(beta-Hydroxyalkanoates) for Potential Applications as Biodegradable Polyesters. Appl Environ Microbiol. 1988; 54(8):1977-82. PMC: 202789. DOI: 10.1128/aem.54.8.1977-1982.1988. View

13.

Farmer W, Liao J . Reduction of aerobic acetate production by Escherichia coli. Appl Environ Microbiol. 1997; 63(8):3205-10. PMC: 168618. DOI: 10.1128/aem.63.8.3205-3210.1997. View

14.

Martin V, Pitera D, Withers S, Newman J, Keasling J . Engineering a mevalonate pathway in Escherichia coli for production of terpenoids. Nat Biotechnol. 2003; 21(7):796-802. DOI: 10.1038/nbt833. View

15.

Pan X, Fan Z, Chen W, Ding Y, Luo H, Bao X . Enhanced ethanol production inside carbon-nanotube reactors containing catalytic particles. Nat Mater. 2007; 6(7):507-11. DOI: 10.1038/nmat1916. View

16.

Membrillo-Hernandez J, Echave P, Cabiscol E, Tamarit J, Ros J, Lin E . Evolution of the adhE gene product of Escherichia coli from a functional reductase to a dehydrogenase. Genetic and biochemical studies of the mutant proteins. J Biol Chem. 2000; 275(43):33869-75. DOI: 10.1074/jbc.M005464200. View

17.

Tamarit J, Cabiscol E, Ros J . Identification of the major oxidatively damaged proteins in Escherichia coli cells exposed to oxidative stress. J Biol Chem. 1998; 273(5):3027-32. DOI: 10.1074/jbc.273.5.3027. View

18.

Gorke B, Stulke J . Carbon catabolite repression in bacteria: many ways to make the most out of nutrients. Nat Rev Microbiol. 2008; 6(8):613-24. DOI: 10.1038/nrmicro1932. View

19.

Lu X, Liu Y, Yang Y, Wang S, Wang Q, Wang X . Constructing a synthetic pathway for acetyl-coenzyme A from one-carbon through enzyme design. Nat Commun. 2019; 10(1):1378. PMC: 6435721. DOI: 10.1038/s41467-019-09095-z. View

20.

Liu B, Xiang S, Zhao G, Wang B, Ma Y, Liu W . Efficient production of 3-hydroxypropionate from fatty acids feedstock in Escherichia coli. Metab Eng. 2018; 51:121-130. DOI: 10.1016/j.ymben.2018.10.003. View