Peptidoglycan Derived from MLGA Up-regulates the Expression of Chicken -defensin 9 Without Triggering an Inflammatory Response

Overview

Journal Innate Immun

Publisher Sage Publications

Specialties Allergy & Immunology
Microbiology

Date 2020 Aug 28

PMID 32847443

Citations 7

Authors

Juan Huang

Junhui Li

Qiufen Li

Lin Li

Nianhua Zhu

Xiaowen Xiong

Guanhong Li

Affiliations

Soon will be listed here.

Abstract

Defensins are critical components of the innate immune system and play an important role in the integration of innate and adaptive immune responses. Although information on the immunomodulatory properties of peptidoglycan from bacteria is abundant, little is known about the β-defensin induction effect of peptidoglycan from the probiotic . This study investigated the effect of intact peptidoglycan from MLGA on the induction of avian β-defensin 9 in chicken immune cells and intestinal explants. Peptidoglycan from MLGA dose dependently promoted avian β-defensin 9 mRNA expression in chicken PBMCs, splenocytes, thymocytes, hepatocytes, and chicken embryo jejunum, ileum, and cecum explants and increased the capacity of PBMC or splenocyte lysates to inhibit the growth of Enteritidis. In contrast to the effect of MLGA-derived peptidoglycan, peptidoglycan derived from pathogenic reduced avian β-defensin 9 mRNA expression in chicken PBMCs and splenocytes. The inducible effect of peptidoglycan from MLGA on avian β-defensin 9 expression in PBMCs and splenocytes was observed without activation of the expression of associated pro-inflammatory cytokines IL-1β, IL-8, and IL-12p40, whereas these cytokine expressions were suppressed by peptidoglycan hydrolysate obtained by lysozyme digestion. The results of the present study show the capability of peptidoglycan derived from MLGA to induce the antimicrobial peptide defensin while simultaneously avoiding the deleterious risks of an inflammatory response.

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