A Completely ATPase from Combinatorial Protein Design
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Our understanding of biological chemistry is shaped by the observation that all life comes from other life-as Pasteur put it, . A key step in expanding our biochemical vocabulary is to recapitulate biogenic catalysis using non-natural sequences that did not arise from common ancestry. Here we describe an enzyme designed completely that hydrolyzes ATP. This protein was designed to lack β-sheet structure and is competitively inhibited by magnesium, two traits that are unlike natural ATPases.
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