MiR-197-3p Reduces Epithelial-mesenchymal Transition by Targeting ABCA7 in Ovarian Cancer Cells
Overview
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The present study was designed to explore the role of microRNA-197-3p in regulating the epithelial-mesenchymal cellular transition in ovarian cancer. The results showed that miR-197 to be significantly ( < 0.05) downregulated in human ovarian cancer tissues and cell lines. Overexpression of miR-197 significantly ( < 0.05) reduced the proliferation of OVACAR-3 cancer cells. Additionally, the colony formation of the OVACAR-3 cells was inhibited by 59% relative to control. The migration and invasion of the OVACAR-3 cells was inhibited by 64% and 72%, respectively, upon miR-197 overexpression. Western blot analysis showed miR-197 was found to upregulate the expression of E-cadherin, while the expression of N-cadherin, vimentin, and snail proteins was found to decrease significantly ( < 0.05). TargetScan analysis together with dual luciferase assay revealed that miR-197 exerts its effects by targeting ABCA7 in ovarian cancer. ABCA7 was significantly ( < 0.05) overexpressed in ovarian cancer tissues and cell lines. However, silencing of ABCA7 resulted in significant inhibition of cell proliferation, migration, and invasion. Nonetheless, overexpression of ABCA7 could abolish the tumor-suppressive effects of miR-197 on the OVACAR-3 cells. Taken together, miR-197 acts a tumor-suppressive in ovarian cancer and points towards its therapeutic implications in the treatment of ovarian cancer.
Guerrero-Orriach J, Carmona-Luque M, Rodriguez-Capitan M, Quesada-Munoz G Pharmaceuticals (Basel). 2025; 18(2).
PMID: 40005961 PMC: 11858145. DOI: 10.3390/ph18020146.
de Bakker T, Maes A, Dragan T, Martinive P, Penninckx S, Van Gestel D Cells. 2025; 14(1.
PMID: 39791719 PMC: 11719474. DOI: 10.3390/cells14010018.
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Fang K, Fang D, Yu H, Chen Y, Yu P, Wang Z Transl Androl Urol. 2024; 13(5):812-827.
PMID: 38855608 PMC: 11157407. DOI: 10.21037/tau-23-469.
Vera-Chang M, Danforth J, Stuart M, Goodarzi A, Brand M, Richardson R Clin Epigenetics. 2023; 15(1):174.
PMID: 37891670 PMC: 10612361. DOI: 10.1186/s13148-023-01564-z.
Wu X, Zhou S, Wang L, Ma J, Zhou Y, Ruan Y Mol Biotechnol. 2023; 66(9):2455-2466.
PMID: 37740818 DOI: 10.1007/s12033-023-00874-0.