Purified Astaxanthin from Promotes Tissue Regeneration by Reducing Oxidative Stress and the Secretion of Collagen and

Overview

Journal Oxid Med Cell Longev

Publisher Wiley

Specialties Cell Biology
Endocrinology

Date 2020 Aug 25

PMID 32832000

Citations 7

Authors

Hsin-Yu Chou

Dik-Lung Ma

Chung-Hang Leung

Chien-Chih Chiu

Tzyh-Chyuan Hour

Hui-Min David Wang

Affiliations

Soon will be listed here.

Abstract

Intracellular reactive apoptosis and reactive oxygen species (ROS) play a crucial role in ultraviolet- (UV-) induced inflammation and aging reaction in human dermal tissues. This study determines the mechanism by which extracts (HPE) and purified astaxanthin (HPA) to promote skin regeneration in the injured tissue and . The results show that HPE and HPA decrease the DNA damage and promote the secretion of collagen from the human normal fibroblast cell line (Hs68) in a dose-dependent manner. UV irradiation and HPA reduce oxidative stress damage due to phorbol-12-myristate-13-acetate (PMA). When skin cells are injured by free radicals, cells undergo a programmed cellular death. Cellular apoptotic death is determined using annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) double staining to verify that there is no cell membrane asymmetry and that the nuclear membrane is broken. Inflammatory symptoms and apoptotic injuries to experimental rats in a group that is treated with HPA treated are decreased in a dose-dependent manner after UVB exposure (300 mJ/cm) for 15 min , compared to the vehicle control group. These positive results show that HPA repairs UVB-triggered skin tissue injury and aging by conducting electrons out of cells to maintain a low level of oxidative stress so that collagen is synthesized and .

Citing Articles

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References

Liu J, Sun Z, Gerken H, Huang J, Jiang Y, Chen F . Genetic engineering of the green alga Chlorella zofingiensis: a modified norflurazon-resistant phytoene desaturase gene as a dominant selectable marker. Appl Microbiol Biotechnol. 2014; 98(11):5069-79. DOI: 10.1007/s00253-014-5593-y. View

Song X, Wang M, Zhang L, Zhang J, Wang X, Liu W . Changes in cell ultrastructure and inhibition of JAK1/STAT3 signaling pathway in CBRH-7919 cells with astaxanthin. Toxicol Mech Methods. 2012; 22(9):679-86. DOI: 10.3109/15376516.2012.717119. View

Zhang L, Liao C, Yang Y, Wang Y, Ding K, Huo D . Response of lipid biosynthesis in Chlorella pyrenoidosa to intracellular reactive oxygen species level under stress conditions. Bioresour Technol. 2019; 287:121414. DOI: 10.1016/j.biortech.2019.121414. View

Hseu Y, Chou C, Kumar K, Fu K, Wang H, Hsu L . Ellagic acid protects human keratinocyte (HaCaT) cells against UVA-induced oxidative stress and apoptosis through the upregulation of the HO-1 and Nrf-2 antioxidant genes. Food Chem Toxicol. 2012; 50(5):1245-55. DOI: 10.1016/j.fct.2012.02.020. View

Wang H, Chiu C, Wu P, Chen C . Subamolide E from Cinnamomum subavenium induces sub-G1 cell-cycle arrest and caspase-dependent apoptosis and reduces the migration ability of human melanoma cells. J Agric Food Chem. 2011; 59(15):8187-92. DOI: 10.1021/jf2018929. View

Schulz I, Mahler H, Boiteux S, Epe B . Oxidative DNA base damage induced by singlet oxygen and photosensitization: recognition by repair endonucleases and mutagenicity. Mutat Res. 2000; 461(2):145-56. DOI: 10.1016/s0921-8777(00)00049-5. View

Wang H, Chen C, Chen C, Ho M, Chou Y, Chang H . (-)-N-Formylanonaine from Michelia alba as a human tyrosinase inhibitor and antioxidant. Bioorg Med Chem. 2010; 18(14):5241-7. DOI: 10.1016/j.bmc.2010.05.045. View

Arakaki A, Matsumoto T, Tateishi T, Matsumoto M, Nojima D, Tomoko Y . UV-C irradiation accelerates neutral lipid synthesis in the marine oleaginous diatom Fistulifera solaris. Bioresour Technol. 2017; 245(Pt B):1520-1526. DOI: 10.1016/j.biortech.2017.05.188. View

Liu L, Wang W, Huang S, Hong Y, Li G, Lin S . Inhibition of the Ras/Raf interaction and repression of renal cancer xenografts by an enantiomeric iridium(iii) metal-based compound. Chem Sci. 2017; 8(7):4756-4763. PMC: 5603957. DOI: 10.1039/c7sc00311k. View

10.

Shen X, Zhao C, Lu J, Guo M . Physicochemical Properties of Whey-Protein-Stabilized Astaxanthin Nanodispersion and Its Transport via a Caco-2 Monolayer. J Agric Food Chem. 2018; 66(6):1472-1478. DOI: 10.1021/acs.jafc.7b05284. View

11.

Hu W, Lee S, Jung M, Heo S, Hur J, Wang M . Induction of cell cycle arrest and apoptosis by the ethyl acetate fraction of Kalopanax pictus leaves in human colon cancer cells. Bioresour Technol. 2010; 101(23):9366-72. DOI: 10.1016/j.biortech.2010.06.091. View

12.

Lin L, Chen C, Kuo C, Lin Y, Hwang B, Wang T . 36H: A Novel Potent Inhibitor for Antimelanogenesis. Oxid Med Cell Longev. 2018; 2018:6354972. PMC: 5817369. DOI: 10.1155/2018/6354972. View

13.

Choi W, Miyamura Y, Wolber R, Smuda C, Reinhold W, Liu H . Regulation of human skin pigmentation in situ by repetitive UV exposure: molecular characterization of responses to UVA and/or UVB. J Invest Dermatol. 2010; 130(6):1685-96. PMC: 3478754. DOI: 10.1038/jid.2010.5. View

14.

Lin C, Chen C, Lin T, Tung J, Wang S . Anti-inflammation activity of fruit essential oil from Cinnamomum insularimontanum Hayata. Bioresour Technol. 2008; 99(18):8783-7. DOI: 10.1016/j.biortech.2008.04.041. View

15.

Chen C, Chiu C, Wu C, Chou Y, Wang H . Enhancements of skin cell proliferations and migrations via 6-dehydrogingerdione. J Agric Food Chem. 2012; 61(6):1349-56. DOI: 10.1021/jf304340q. View

16.

Hsin-Ti L, Wen-Sheng L, Yi-Chia W, Ya-Wei L, Wen Z, David W . The Effect in Topical Use of Lycogen(TM) via Sonophoresis for Anti-aging on Facial Skin. Curr Pharm Biotechnol. 2015; 16(12):1063-9. DOI: 10.2174/1389201016666150731112010. View

17.

Chen R, Zhang Y, Lan J, Liu H, Li W, Wu Y . Ischemic Postconditioning Alleviates Intestinal Ischemia-Reperfusion Injury by Enhancing Autophagy and Suppressing Oxidative Stress through the Akt/GSK-3/Nrf2 Pathway in Mice. Oxid Med Cell Longev. 2020; 2020:6954764. PMC: 7102478. DOI: 10.1155/2020/6954764. View

18.

Che H, Li Q, Zhang T, Wang D, Yang L, Xu J . Effects of Astaxanthin and Docosahexaenoic-Acid-Acylated Astaxanthin on Alzheimer's Disease in APP/PS1 Double-Transgenic Mice. J Agric Food Chem. 2018; 66(19):4948-4957. DOI: 10.1021/acs.jafc.8b00988. View

19.

Wu P, Chiu C, Chen C, Wang H . 7-Hydroxydehydronuciferine induces human melanoma death via triggering autophagy and apoptosis. Exp Dermatol. 2015; 24(12):930-5. DOI: 10.1111/exd.12805. View

20.

Ma D, Liu L, Leung K, Chen Y, Zhong H, Chan D . Antagonizing STAT3 dimerization with a rhodium(III) complex. Angew Chem Int Ed Engl. 2014; 53(35):9178-82. DOI: 10.1002/anie.201404686. View