The Yeast SNF3 Gene Encodes a Glucose Transporter Homologous to the Mammalian Protein

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1988 Apr 1

PMID 3281163

Citations 66

Authors

J L Celenza

M Carlson

Affiliations

Soon will be listed here.

Abstract

The SNF3 gene is required for high-affinity glucose transport in the yeast Saccharomyces cerevisiae and has also been implicated in control of gene expression by glucose repression. We report here the nucleotide sequence of the cloned SNF3 gene. The predicted amino acid sequence shows that SNF3 encodes a 97-kilodalton protein that is homologous to mammalian glucose transporters and has 12 putative membrane-spanning regions. We also show that a functional SNF3-lacZ gene-fusion product cofractionates with membrane proteins and is localized to the cell surface, as judged by indirect immunofluorescence microscopy. Expression of the fusion protein is regulated by glucose repression.

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