Yeast STE7, STE11, and STE12 Genes Are Required for Expression of Cell-type-specific Genes

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1988 Feb 1

PMID 3280969

Citations 44

Authors

S Fields

D T Chaleff

G F Sprague Jr

Affiliations

Soon will be listed here.

Abstract

Cell type specialization in yeast haploids involves the mutually exclusive expression of one of two sets of genes, the a-specific and alpha-specific genes. We demonstrated that the products of the STE7, STE11, and STE12 genes were required for the expression of both gene sets. RNA levels transcribed from these gene sets were significantly decreased but not abolished in haploids containing a null mutation in the STE7, STE11, or STE12 gene. Transcript levels from the a- and alpha-specific gene sets were not further reduced in strains harboring mutations in all three STE genes, suggesting that STE7, STE11, and STE12 are required for the same aspect of transcription. We further showed that the requirement for these products was not the same for each member of a particular gene set. However, for any given a- or alpha-specific gene, the effect on RNA levels of any of the three ste mutations was similar.

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References

Rigby P, Dieckmann M, Rhodes C, Berg P . Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977; 113(1):237-51. DOI: 10.1016/0022-2836(77)90052-3. View

Johnson A, Herskowitz I . A repressor (MAT alpha 2 Product) and its operator control expression of a set of cell type specific genes in yeast. Cell. 1985; 42(1):237-47. DOI: 10.1016/s0092-8674(85)80119-7. View

Thomas P . Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980; 77(9):5201-5. PMC: 350025. DOI: 10.1073/pnas.77.9.5201. View

Wilson K, Herskowitz I . Negative regulation of STE6 gene expression by the alpha 2 product of Saccharomyces cerevisiae. Mol Cell Biol. 1984; 4(11):2420-7. PMC: 369073. DOI: 10.1128/mcb.4.11.2420-2427.1984. View

Mackay V, MANNEY T . Mutations affecting sexual conjugation and related processes in Saccharomyces cerevisiae. I. Isolation and phenotypic characterization of nonmating mutants. Genetics. 1974; 76(2):255-71. PMC: 1213064. DOI: 10.1093/genetics/76.2.255. View

Sprague Jr G, Blair L, Thorner J . Cell interactions and regulation of cell type in the yeast Saccharomyces cerevisiae. Annu Rev Microbiol. 1983; 37:623-60. DOI: 10.1146/annurev.mi.37.100183.003203. View

Nakayama N, Miyajima A, Arai K . Nucleotide sequences of STE2 and STE3, cell type-specific sterile genes from Saccharomyces cerevisiae. EMBO J. 1985; 4(10):2643-8. PMC: 554555. DOI: 10.1002/j.1460-2075.1985.tb03982.x. View

Dubois E, Jacobs E, Jauniaux J . Expression of the ROAM mutations in Saccharomyces cerevisiae: involvement of trans-acting regulatory elements and relation with the Ty1 transcription. EMBO J. 1982; 1(9):1133-9. PMC: 553174. DOI: 10.1002/j.1460-2075.1982.tb01308.x. View

Fields S, Herskowitz I . The yeast STE12 product is required for expression of two sets of cell-type specific genes. Cell. 1985; 42(3):923-30. DOI: 10.1016/0092-8674(85)90288-0. View

10.

Jensen R, Sprague Jr G, Herskowitz I . Regulation of yeast mating-type interconversion: feedback control of HO gene expression by the mating-type locus. Proc Natl Acad Sci U S A. 1983; 80(10):3035-9. PMC: 393968. DOI: 10.1073/pnas.80.10.3035. View

11.

Strathern J, Hicks J, Herskowitz I . Control of cell type in yeast by the mating type locus. The alpha 1-alpha 2 hypothesis. J Mol Biol. 1981; 147(3):357-72. DOI: 10.1016/0022-2836(81)90488-5. View

12.

Hartwell L . Mutants of Saccharomyces cerevisiae unresponsive to cell division control by polypeptide mating hormone. J Cell Biol. 1980; 85(3):811-22. PMC: 2111434. DOI: 10.1083/jcb.85.3.811. View

13.

Bender A, Sprague Jr G . Yeast peptide pheromones, a-factor and alpha-factor, activate a common response mechanism in their target cells. Cell. 1986; 47(6):929-37. DOI: 10.1016/0092-8674(86)90808-1. View

14.

Mitchell A, Herskowitz I . Activation of meiosis and sporulation by repression of the RME1 product in yeast. Nature. 1986; 319(6056):738-42. DOI: 10.1038/319738a0. View

15.

Sprague Jr G, Jensen R, Herskowitz I . Control of yeast cell type by the mating type locus: positive regulation of the alpha-specific STE3 gene by the MAT alpha 1 product. Cell. 1983; 32(2):409-15. DOI: 10.1016/0092-8674(83)90460-9. View

16.

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

17.

Mackay V, MANNEY T . Mutations affecting sexual conjugation and related processes in Saccharomyces cerevisiae. II. Genetic analysis of nonmating mutants. Genetics. 1974; 76(2):273-88. PMC: 1213065. DOI: 10.1093/genetics/76.2.273. View

18.

Jenness D, Goldman B, Hartwell L . Saccharomyces cerevisiae mutants unresponsive to alpha-factor pheromone: alpha-factor binding and extragenic suppression. Mol Cell Biol. 1987; 7(4):1311-9. PMC: 365215. DOI: 10.1128/mcb.7.4.1311-1319.1987. View

19.

Chaleff D, Tatchell K . Molecular cloning and characterization of the STE7 and STE11 genes of Saccharomyces cerevisiae. Mol Cell Biol. 1985; 5(8):1878-86. PMC: 366903. DOI: 10.1128/mcb.5.8.1878-1886.1985. View

20.

Rothstein R . One-step gene disruption in yeast. Methods Enzymol. 1983; 101:202-11. DOI: 10.1016/0076-6879(83)01015-0. View