Electrochemical Immunosensor with Cu(I)/Cu(II)-chitosan-graphene Nanocomposite-based Signal Amplification for the Detection of Newcastle Disease Virus

Overview

Journal Sci Rep

Specialty Science

Date 2020 Aug 19

PMID 32807824

Citations 5

Authors

Jiaoling Huang

Zhixun Xie

Yihong Huang

Liji Xie

Sisi Luo

Qing Fan

TingTing Zeng

Yanfang Zhang

Sheng Wang

Minxiu Zhang

Zhiqin Xie

Xianwen Deng

Affiliations

Soon will be listed here.

Abstract

An electrochemical immunoassay for the ultrasensitive detection of Newcastle disease virus (NDV) was developed using graphene and chitosan-conjugated Cu(I)/Cu(II) (Cu(I)/Cu(II)-Chi-Gra) for signal amplification. Graphene (Gra) was used for both the conjugation of an anti-Newcastle disease virus monoclonal antibody (MAb/NDV) and the immobilization of anti-Newcastle disease virus polyclonal antibodies (PAb/NDV). Cu(I)/Cu(II) was selected as an electroactive probe, immobilized on a chitosan-graphene (Chi-Gra) hybrid material, and detected by differential pulse voltammetry (DPV) after a sandwich-type immune response. Because Gra had a large surface area, many antibodies were loaded onto the electrochemical immunosensor to effectively increase the electrical signal. Additionally, the introduction of Gra significantly increased the loading amount of electroactive probes (Cu(I)/Cu(II)), and the electrical signal was further amplified. Cu(I)/Cu(II) and Cu(I)/Cu(II)-Chi-Gra were compared in detail to characterize the signal amplification ability of this platform. The results showed that this immunosensor exhibited excellent analytical performance in the detection of NDV in the concentration range of 10 to 10 EID/0.1 mL, and it had a detection limit of 10 EID/0.1 mL, which was calculated based on a signal-to-noise (S/N) ratio of 3. The resulting immunosensor also exhibited high sensitivity, good reproducibility and acceptable stability.

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References

Brown V, Bevins S . A review of virulent Newcastle disease viruses in the United States and the role of wild birds in viral persistence and spread. Vet Res. 2017; 48(1):68. PMC: 5659000. DOI: 10.1186/s13567-017-0475-9. View

Ganar K, Das M, Sinha S, Kumar S . Newcastle disease virus: current status and our understanding. Virus Res. 2014; 184:71-81. PMC: 7127793. DOI: 10.1016/j.virusres.2014.02.016. View

Fratnik Steyer A, Zorman Rojs O, Krapez U, Slavec B, Barlic-Maganja D . A diagnostic method based on MGB probes for rapid detection and simultaneous differentiation between virulent and vaccine strains of avian paramyxovirus type 1. J Virol Methods. 2010; 166(1-2):28-36. DOI: 10.1016/j.jviromet.2010.02.012. View

Li Q, Wang L, Sun Y, Liu J, Ma F, Yang J . Evaluation of an immunochromatographic strip for detection of avian avulavirus 1 (Newcastle disease virus). J Vet Diagn Invest. 2019; 31(3):475-480. PMC: 6838702. DOI: 10.1177/1040638719837320. View

Hosu O, Selvolini G, Cristea C, Marrazza G . Electrochemical Immunosensors for Disease Detection and Diagnosis. Curr Med Chem. 2017; 25(33):4119-4137. DOI: 10.2174/0929867324666170727104429. View

Felix F, Angnes L . Electrochemical immunosensors - A powerful tool for analytical applications. Biosens Bioelectron. 2017; 102:470-478. DOI: 10.1016/j.bios.2017.11.029. View

Aydin E, Aydin M, Sezginturk M . Electrochemical immunosensor based on chitosan/conductive carbon black composite modified disposable ITO electrode: An analytical platform for p53 detection. Biosens Bioelectron. 2018; 121:80-89. DOI: 10.1016/j.bios.2018.09.008. View

Aydin M, Aydin E, Sezginturk M . A highly selective electrochemical immunosensor based on conductive carbon black and star PGMA polymer composite material for IL-8 biomarker detection in human serum and saliva. Biosens Bioelectron. 2018; 117:720-728. DOI: 10.1016/j.bios.2018.07.010. View

Wang B, Ji X, Zhao H, Wang N, Li X, Ni R . An amperometric β-glucan biosensor based on the immobilization of bi-enzyme on Prussian blue-chitosan and gold nanoparticles-chitosan nanocomposite films. Biosens Bioelectron. 2013; 55:113-9. DOI: 10.1016/j.bios.2013.12.004. View

10.

Bhattarai J, Neupane D, Nepal B, Mikhaylov V, Demchenko A, Stine K . Preparation, Modification, Characterization, and Biosensing Application of Nanoporous Gold Using Electrochemical Techniques. Nanomaterials (Basel). 2018; 8(3). PMC: 5869662. DOI: 10.3390/nano8030171. View

11.

Sun D, Li H, Li M, Li C, Qian L, Yang B . Electrochemical immunosensors with AuPt-vertical graphene/glassy carbon electrode for alpha-fetoprotein detection based on label-free and sandwich-type strategies. Biosens Bioelectron. 2019; 132:68-75. DOI: 10.1016/j.bios.2019.02.045. View

12.

Rezaei B, Shoushtari A, Rabiee M, Uzun L, Mak W, Turner A . An electrochemical immunosensor for cardiac Troponin I using electrospun carboxylated multi-walled carbon nanotube-whiskered nanofibres. Talanta. 2018; 182:178-186. DOI: 10.1016/j.talanta.2018.01.046. View

13.

Sun B, Gou Y, Ma Y, Zheng X, Bai R, Abdelmoaty A . Investigate electrochemical immunosensor of cortisol based on gold nanoparticles/magnetic functionalized reduced graphene oxide. Biosens Bioelectron. 2016; 88:55-62. DOI: 10.1016/j.bios.2016.07.047. View

14.

Tuteja S, Chen R, Kukkar M, Song C, Mutreja R, Singh S . A label-free electrochemical immunosensor for the detection of cardiac marker using graphene quantum dots (GQDs). Biosens Bioelectron. 2016; 86:548-556. DOI: 10.1016/j.bios.2016.07.052. View

15.

Bhardwaj H, Pandey M, Rajesh , Sumana G . Electrochemical Aflatoxin B1 immunosensor based on the use of graphene quantum dots and gold nanoparticles. Mikrochim Acta. 2019; 186(8):592. DOI: 10.1007/s00604-019-3701-5. View

16.

Geim A, Novoselov K . The rise of graphene. Nat Mater. 2007; 6(3):183-91. DOI: 10.1038/nmat1849. View

17.

Liu J, Wang J, Wang T, Li D, Xi F, Wang J . Three-dimensional electrochemical immunosensor for sensitive detection of carcinoembryonic antigen based on monolithic and macroporous graphene foam. Biosens Bioelectron. 2014; 65:281-6. DOI: 10.1016/j.bios.2014.10.016. View

18.

Li L, Zhang L, Yu J, Ge S, Song X . All-graphene composite materials for signal amplification toward ultrasensitive electrochemical immunosensing of tumor marker. Biosens Bioelectron. 2015; 71:108-114. DOI: 10.1016/j.bios.2015.04.032. View

19.

Huang J, Xie Z, Xie Z, Luo S, Xie L, Huang L . Silver nanoparticles coated graphene electrochemical sensor for the ultrasensitive analysis of avian influenza virus H7. Anal Chim Acta. 2016; 913:121-7. DOI: 10.1016/j.aca.2016.01.050. View

20.

Guzman J, Saucedo I, Revilla J, Navarro R, Guibal E . Copper sorption by chitosan in the presence of citrate ions: influence of metal speciation on sorption mechanism and uptake capacities. Int J Biol Macromol. 2003; 33(1-3):57-65. DOI: 10.1016/s0141-8130(03)00067-9. View