Widespread Protein Lysine Acetylation in Gut Microbiome and Its Alterations in Patients with Crohn's Disease

Overview

Journal Nat Commun

Specialty Biology

Date 2020 Aug 19

PMID 32807798

Citations 30

Authors

Xu Zhang

Zhibin Ning

Janice Mayne

Yidai Yang

Shelley A Deeke

Krystal Walker

Charles L Farnsworth

Matthew P Stokes

Jean-Francois Couture

David Mack

Alain Stintzi

Daniel Figeys

Affiliations

Soon will be listed here.

Abstract

Lysine acetylation (Kac), an abundant post-translational modification (PTM) in prokaryotes, regulates various microbial metabolic pathways. However, no studies have examined protein Kac at the microbiome level, and it remains unknown whether Kac level is altered in patient microbiomes. Herein, we use a peptide immuno-affinity enrichment strategy coupled with mass spectrometry to characterize protein Kac in the microbiome, which successfully identifies 35,200 Kac peptides from microbial or human proteins in gut microbiome samples. We demonstrate that Kac is widely distributed in gut microbial metabolic pathways, including anaerobic fermentation to generate short-chain fatty acids. Applying to the analyses of microbiomes of patients with Crohn's disease identifies 52 host and 136 microbial protein Kac sites that are differentially abundant in disease versus controls. This microbiome-wide acetylomic approach aids in advancing functional microbiome research.

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