The Uptake of Ivermectin and Its Effects in Roots, Leaves and Seeds of Soybean ()

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2020 Aug 16

PMID 32796616

Citations 2

Authors

Martina Navratilova

Lucie Raisova Stuchlikova

Katerina Motkova

Barbora Szotakova

Lenka Skalova

Lenka Langhansova

Radka Podlipna

Affiliations

Soon will be listed here.

Abstract

In recent years interest has grown in the occurrence and the effects of pharmaceuticals in the environment. The aim of this work is to evaluate the risk of fertilizing crops with manure from livestock treated with anthelmintics. The present study was designed to follow the fate of the commonly used anthelmintic drug, ivermectin (IVM) and its metabolites in soybeans ( (L.) Merr.), a plant that is grown and consumed world-wide for its high content of nutritional and health-beneficial substances. In vitro plantlets and soybean plants, cultivated in a greenhouse, were used for this purpose. Our results showed the uptake of IVM and its translocation to the leaves, but not in the pods and the beans. Four IVM metabolites were detected in the roots, and one in the leaves. IVM exposure decreased slightly the number and weight of the beans and induced changes in the activities of antioxidant enzymes. In addition, the presence of IVM affected the proportion of individual isoflavones and reduced the content of isoflavones aglycones, which might decrease the therapeutic value of soybeans. Fertilization of soybean fields with manure from IVM-treated animals appears to be safe for humans, due to the absence of IVM in beans, the food part of plants. On the other hand, it could negatively affect soybean plants and herbivorous invertebrates.

Citing Articles

Temperature moderates impact of formulated moxidectin on seed germination of three temperate grassland species.

Eichberg C, Hartman A, Kronenberger A, During R, Donath T PLoS One. 2022; 17(11):e0277865.

PMID: 36409735 PMC: 9678283. DOI: 10.1371/journal.pone.0277865.

Lipidomics-Based Comparison of Molecular Compositions of Green, Yellow, and Red Bell Peppers.

Sutliff A, Saint-Cyr M, Hendricks A, Chen S, Doenges K, Quinn K Metabolites. 2021; 11(4).

PMID: 33919953 PMC: 8070949. DOI: 10.3390/metabo11040241.

References

Iglesias L, Saumell C, Sagues F, Sallovitz J, Lifschitz A . Ivermectin dissipation and movement from feces to soil under field conditions. J Environ Sci Health B. 2017; 53(1):42-48. DOI: 10.1080/03601234.2017.1371554. View

Vokral I, Michaela S, Radka P, Jiri L, Lukas P, Dominika S . Ivermectin environmental impact: Excretion profile in sheep and phytotoxic effect in Sinapis alba. Ecotoxicol Environ Saf. 2019; 169:944-949. DOI: 10.1016/j.ecoenv.2018.11.097. View

Svobodnikova L, Kummerova M, Zezulka S, Babula P . Possible use of a Nicotiana tabacum 'Bright Yellow 2' cell suspension as a model to assess phytotoxicity of pharmaceuticals (diclofenac). Ecotoxicol Environ Saf. 2019; 182:109369. DOI: 10.1016/j.ecoenv.2019.109369. View

Singh V, Pandey B, Suthar S . Phytotoxicity and degradation of antibiotic ofloxacin in duckweed (Spirodela polyrhiza) system. Ecotoxicol Environ Saf. 2019; 179:88-95. DOI: 10.1016/j.ecoenv.2019.04.018. View

Jaeger L, Carvalho-Costa F . Status of benzimidazole resistance in intestinal nematode populations of livestock in Brazil: a systematic review. BMC Vet Res. 2017; 13(1):358. PMC: 5702221. DOI: 10.1186/s12917-017-1282-2. View

Carvalho P, Basto M, Almeida C, Brix H . A review of plant-pharmaceutical interactions: from uptake and effects in crop plants to phytoremediation in constructed wetlands. Environ Sci Pollut Res Int. 2014; 21(20):11729-63. DOI: 10.1007/s11356-014-2550-3. View

Gutierrez-Gonzalez J, Guttikonda S, Tran L, Aldrich D, Zhong R, Yu O . Differential expression of isoflavone biosynthetic genes in soybean during water deficits. Plant Cell Physiol. 2010; 51(6):936-48. DOI: 10.1093/pcp/pcq065. View

Syslova E, Landa P, Raisova Stuchlikova L, Matouskova P, Skalova L, Szotakova B . Metabolism of the anthelmintic drug fenbendazole in Arabidopsis thaliana and its effect on transcriptome and proteome. Chemosphere. 2018; 218:662-669. DOI: 10.1016/j.chemosphere.2018.11.135. View

Ismail M, Botros S, Metwally A, William S, Farghally A, Tao L . Resistance to praziquantel: direct evidence from Schistosoma mansoni isolated from Egyptian villagers. Am J Trop Med Hyg. 1999; 60(6):932-5. DOI: 10.4269/ajtmh.1999.60.932. View

10.

Madikizela L, Ncube S, Chimuka L . Uptake of pharmaceuticals by plants grown under hydroponic conditions and natural occurring plant species: A review. Sci Total Environ. 2018; 636:477-486. DOI: 10.1016/j.scitotenv.2018.04.297. View

11.

Chitescu C, Nicolau A, Romkens P, van der Fels-Klerx H . Quantitative modelling to estimate the transfer of pharmaceuticals through the food production system. J Environ Sci Health B. 2014; 49(7):457-67. DOI: 10.1080/03601234.2014.896659. View

12.

Syslova E, Landa P, Navratilova M, Raisova Stuchlikova L, Matouskova P, Skalova L . Ivermectin biotransformation and impact on transcriptome in Arabidopsis thaliana. Chemosphere. 2019; 234:528-535. DOI: 10.1016/j.chemosphere.2019.06.102. View

13.

Gamborg O, Miller R, Ojima K . Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res. 1968; 50(1):151-8. DOI: 10.1016/0014-4827(68)90403-5. View

14.

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

15.

Marsik P, Podlipna R, Vanek T . Study of praziquantel phytoremediation and transformation and its removal in constructed wetland. J Hazard Mater. 2016; 323(Pt A):394-399. DOI: 10.1016/j.jhazmat.2016.05.045. View

16.

Kawakami Y, Tsurugasaki W, Nakamura S, Osada K . Comparison of regulative functions between dietary soy isoflavones aglycone and glucoside on lipid metabolism in rats fed cholesterol. J Nutr Biochem. 2005; 16(4):205-12. DOI: 10.1016/j.jnutbio.2004.11.005. View

17.

Liu J, Hu B, Liu W, Qin W, Wu H, Zhang J . Metabolomic tool to identify soybean [Glycine max (L.) Merrill] germplasms with a high level of shade tolerance at the seedling stage. Sci Rep. 2017; 7:42478. PMC: 5304147. DOI: 10.1038/srep42478. View

18.

Bartikova H, Podlipna R, Skalova L . Veterinary drugs in the environment and their toxicity to plants. Chemosphere. 2015; 144:2290-301. DOI: 10.1016/j.chemosphere.2015.10.137. View

19.

Raisova Stuchlikova L, Skalova L, Szotakova B, Syslova E, Vokral I, Vanek T . Biotransformation of flubendazole and fenbendazole and their effects in the ribwort plantain (Plantago lanceolata). Ecotoxicol Environ Saf. 2017; 147:681-687. DOI: 10.1016/j.ecoenv.2017.09.020. View

20.

Stuchlikova Raisova L, Podlipna R, Szotakova B, Syslova E, Skalova L . Evaluation of drug uptake and deactivation in plant: Fate of albendazole in ribwort plantain (Plantago laceolata) cells and regenerants. Ecotoxicol Environ Saf. 2017; 141:37-42. DOI: 10.1016/j.ecoenv.2017.03.014. View