Endosidin 2 Accelerates PIN2 Endocytosis and Disturbs Intracellular Trafficking of PIN2, PIN3, and PIN4 but Not of SYT1

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2020 Aug 14

PMID 32790800

Citations 7

Authors

Alexandra Leskova

Maria Labajova

Miroslav Krausko

Alexandra Zahradnikova

Frantisek Baluska

Karol Micieta

Jan Turna

Jan Jasik

Affiliations

Soon will be listed here.

Abstract

We established that Endosidin2 (ES2) affected the trafficking routes of both newly synthesized and endocytic pools of PIN-FORMED2 (PIN2) in Arabidopsis root epidermal cells. PIN2 populations accumulated in separated patches, which gradually merged into large and compact ES2 aggregates (ES2As). FM4-64 endocytic tracer labeled ES2As as well. Both PIN2 pools also appeared in vacuoles. Accelerated endocytosis of PIN2, its aggregation in the cytoplasm, and redirection of PIN2 flows to vacuoles led to a substantial reduction of the abundance of this protein in the plasma membrane. Whereas PIN-FORMED3 and PIN-FORMED4 also aggregated in the cytoplasm, SYT1 was not sensitive to ES2 treatment and did not appear either in the cytoplasmic aggregates or vacuoles. Ultrastructural analysis revealed that ES2 affects the Golgi apparatus so that stacks acquired cup-shape and even circular shape surrounded by several vesicles. Abnormally shaped Golgi stacks, stack remnants, multi-lamellar structures, separated Golgi cisterna rings, tubular structures, and vesicles formed discrete clusters.

Citing Articles

SYNAPTOTAGMIN 4 is expressed mainly in the phloem and participates in abiotic stress tolerance in Arabidopsis.

Kumar A, Krausko M, Jasik J Front Plant Sci. 2024; 15:1363555.

PMID: 39011301 PMC: 11246894. DOI: 10.3389/fpls.2024.1363555.

A chemical genetic screen with the EXO70 inhibitor Endosidin2 uncovers potential modulators of exocytosis in Arabidopsis.

Li X, Wang D, Yin X, Dai M, Staiger C, Zhang C Plant Direct. 2024; 8(6):e592.

PMID: 38881683 PMC: 11176578. DOI: 10.1002/pld3.592.

The EXO70 inhibitor Endosidin2 alters plasma membrane protein composition in Arabidopsis roots.

Li X, Zhu P, Chen Y, Huang L, Wang D, Newton D Front Plant Sci. 2023; 14:1171957.

PMID: 37324680 PMC: 10264680. DOI: 10.3389/fpls.2023.1171957.

Inhibition of the Exocyst Complex with Endosidin 2 Reduces Polarized Growth in .

Bormann E, Xu R, Nargi C, Wu M, Vidali L MicroPubl Biol. 2022; 2022.

PMID: 36411798 PMC: 9674970. DOI: 10.17912/micropub.biology.000655.

Exocyst inactivation in urothelial cells disrupts autophagy and activates non-canonical NF-κB signaling.

Ortega M, Villiger R, Harrison-Chau M, Lieu S, Tamashiro K, Lee A Dis Model Mech. 2022; 15(10).

PMID: 36004645 PMC: 9586569. DOI: 10.1242/dmm.049785.

References

Jasik J, Boggetti B, Baluska F, Volkmann D, Gensch T, Rutten T . PIN2 turnover in Arabidopsis root epidermal cells explored by the photoconvertible protein Dendra2. PLoS One. 2013; 8(4):e61403. PMC: 3630207. DOI: 10.1371/journal.pone.0061403. View

Jasik J, Bokor B, Stuchlik S, Micieta K, Turna J, Schmelzer E . Effects of Auxins on PIN-FORMED2 (PIN2) Dynamics Are Not Mediated by Inhibiting PIN2 Endocytosis. Plant Physiol. 2016; 172(2):1019-1031. PMC: 5047079. DOI: 10.1104/pp.16.00563. View

Viotti C, Bubeck J, Stierhof Y, Krebs M, Langhans M, van den Berg W . Endocytic and secretory traffic in Arabidopsis merge in the trans-Golgi network/early endosome, an independent and highly dynamic organelle. Plant Cell. 2010; 22(4):1344-57. PMC: 2879741. DOI: 10.1105/tpc.109.072637. View

Dettmer J, Schubert D, Calvo-Weimar O, Stierhof Y, Schmidt R, Schumacher K . Essential role of the V-ATPase in male gametophyte development. Plant J. 2004; 41(1):117-24. DOI: 10.1111/j.1365-313X.2004.02282.x. View

Drakakaki G, Robert S, Szatmari A, Brown M, Nagawa S, Van Damme D . Clusters of bioactive compounds target dynamic endomembrane networks in vivo. Proc Natl Acad Sci U S A. 2011; 108(43):17850-5. PMC: 3203817. DOI: 10.1073/pnas.1108581108. View

Baluska F, Kubica S, Hauskrecht M . Postmitotic 'isodiametric' cell growth in the maize root apex. Planta. 2013; 181(3):269-74. DOI: 10.1007/BF00195876. View

Ballensiefen W, Ossipov D, Schmitt H . Recycling of the yeast v-SNARE Sec22p involves COPI-proteins and the ER transmembrane proteins Ufe1p and Sec20p. J Cell Sci. 1998; 111 ( Pt 11):1507-20. DOI: 10.1242/jcs.111.11.1507. View

Laxmi A, Pan J, Morsy M, Chen R . Light plays an essential role in intracellular distribution of auxin efflux carrier PIN2 in Arabidopsis thaliana. PLoS One. 2008; 3(1):e1510. PMC: 2200863. DOI: 10.1371/journal.pone.0001510. View

Uemura T, Nakano R, Takagi J, Wang Y, Kramer K, Finkemeier I . A Golgi-Released Subpopulation of the Trans-Golgi Network Mediates Protein Secretion in Arabidopsis. Plant Physiol. 2018; 179(2):519-532. PMC: 6426420. DOI: 10.1104/pp.18.01228. View

10.

Huang L, Zhang C . Use Endosidin2 to Study Plant Exocytosis and Vacuolar Trafficking. Methods Mol Biol. 2018; 1789:167-175. DOI: 10.1007/978-1-4939-7856-4_13. View

11.

Huttlin E, Bruckner R, Paulo J, Cannon J, Ting L, Baltier K . Architecture of the human interactome defines protein communities and disease networks. Nature. 2017; 545(7655):505-509. PMC: 5531611. DOI: 10.1038/nature22366. View

12.

Reynolds E . The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963; 17:208-12. PMC: 2106263. DOI: 10.1083/jcb.17.1.208. View

13.

Robinson D, Langhans M, Saint-Jore-Dupas C, Hawes C . BFA effects are tissue and not just plant specific. Trends Plant Sci. 2008; 13(8):405-8. DOI: 10.1016/j.tplants.2008.05.010. View

14.

Vukasinovic N, Zarsky V . Tethering Complexes in the Arabidopsis Endomembrane System. Front Cell Dev Biol. 2016; 4:46. PMC: 4871884. DOI: 10.3389/fcell.2016.00046. View

15.

Sauer M, Delgadillo M, Zouhar J, Reynolds G, Pennington J, Jiang L . MTV1 and MTV4 encode plant-specific ENTH and ARF GAP proteins that mediate clathrin-dependent trafficking of vacuolar cargo from the trans-Golgi network. Plant Cell. 2013; 25(6):2217-35. PMC: 3723622. DOI: 10.1105/tpc.113.111724. View

16.

Paciorek T, Zazimalova E, Ruthardt N, Petrasek J, Stierhof Y, Kleine-Vehn J . Auxin inhibits endocytosis and promotes its own efflux from cells. Nature. 2005; 435(7046):1251-6. DOI: 10.1038/nature03633. View

17.

Clough S, Bent A . Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 1999; 16(6):735-43. DOI: 10.1046/j.1365-313x.1998.00343.x. View

18.

Liljegren S, Leslie M, Darnielle L, Lewis M, Taylor S, Luo R . Regulation of membrane trafficking and organ separation by the NEVERSHED ARF-GAP protein. Development. 2009; 136(11):1909-18. PMC: 2680113. DOI: 10.1242/dev.033605. View

19.

Wu B, Guo W . The Exocyst at a Glance. J Cell Sci. 2015; 128(16):2957-64. PMC: 4541039. DOI: 10.1242/jcs.156398. View

20.

Formstecher E, Aresta S, Collura V, Hamburger A, Meil A, Trehin A . Protein interaction mapping: a Drosophila case study. Genome Res. 2005; 15(3):376-84. PMC: 551564. DOI: 10.1101/gr.2659105. View