Possible Role of MicroRNA MiRNA-IL-25 Interaction in Mice with Ulcerative Colitis

Overview

Journal Bioengineered

Date 2020 Aug 12

PMID 32779953

Citations 4

Authors

Jun Yao

Ruo-Yu Gao

Ming-Han Luo

Cheng Wei

Ben-Hua Wu

Li-Liangzi Guo

Li-Sheng Wang

Jian-Yao Wang

De-Feng Li

Affiliations

Soon will be listed here.

Abstract

Background: The regulatory network of ulcerative colitis (UC)-associated miRNAs is not fully understood. In this study, we aim to investigate the global profile and regulatory network of UC associated miRNAs in the context of dextran sulfate sodium (DSS).

Methods: UC was induced in C57BL mice using DSS. Differentially expressed miRNAs were screened by RNA sequencing and subjected to the Kyoto Encyclopedia of Genes and Genomes Pathway enrichment analysis. RT-qPCR was used to verify the differential expression of miRNAs and candidate target mRNA. Luciferase reporter vector bearing a miRNA binding site was constructed to verify the binding site of the miRNA on mRNA.

Results: A total of 95 miRNAs (31 were up-regulated and 64 were down regulated) differentially expressed in the colonic tissues of the UC mice. Among the differentially expressed miRNAs, IL-25 pathway genes were enriched. Subsequent RT-qPCR confirmed a decreased expression of IL-25 and a significant up regulation of IL-25 target miRNAs including mmu-miR-135b-5p, mmu-miR-7239-5p and mmu-miR-691 in UC mice.

Conclusion: Using the luciferase assay, we verified the biding sites of mmu-miR-135b-5p and mmu-miR-691 to the IL-25 3'UTR. In conclusion, mmu-miR-135b-5p:IL-25 and mmu-miR-691:IL-25 interactions are important pathways that may exert a protective role in UC.

Citing Articles

Selection and Evaluation of mRNA and miRNA Reference Genes for Expression Studies (qPCR) in Archived Formalin-Fixed and Paraffin-Embedded (FFPE) Colon Samples of DSS-Induced Colitis Mouse Model.

Unkovic A, Bostjancic E, Belic A, Perse M Biology (Basel). 2023; 12(2).

PMID: 36829468 PMC: 9952917. DOI: 10.3390/biology12020190.

Interleukin 25 and its biological features and function in intestinal diseases.

Jiang W, Wang Z, Zhang J, Li M Cent Eur J Immunol. 2023; 47(4):362-372.

PMID: 36817397 PMC: 9901255. DOI: 10.5114/ceji.2022.124416.

Sinomenine ameliorates cardiac hypertrophy by activating Nrf2/ARE signaling pathway.

Yuan M, Zhao B, Jia H, Zhang C, Zuo X Bioengineered. 2021; 12(2):12778-12788.

PMID: 34895050 PMC: 8810090. DOI: 10.1080/21655979.2021.2000195.

Long non-coding RNA SOX21-AS1 modulates lung cancer progress upon microRNA miR-24-3p/PIM2 axis.

Wang F, Gu T, Chen Y, Chen Y, Xiong D, Zhu Y Bioengineered. 2021; 12(1):6724-6737.

PMID: 34511042 PMC: 8806559. DOI: 10.1080/21655979.2021.1955578.

References

Olaru A, Selaru F, Mori Y, Vazquez C, David S, Paun B . Dynamic changes in the expression of MicroRNA-31 during inflammatory bowel disease-associated neoplastic transformation. Inflamm Bowel Dis. 2010; 17(1):221-31. PMC: 3006011. DOI: 10.1002/ibd.21359. View

Yu Q, Saruta M, Papadakis K . Visilizumab induces apoptosis of mucosal T lymphocytes in ulcerative colitis through activation of caspase 3 and 8 dependent pathways. Clin Immunol. 2008; 127(3):322-9. DOI: 10.1016/j.clim.2008.02.009. View

Wu F, Huang Y, Dong F, Kwon J . Ulcerative Colitis-Associated Long Noncoding RNA, BC012900, Regulates Intestinal Epithelial Cell Apoptosis. Inflamm Bowel Dis. 2016; 22(4):782-95. DOI: 10.1097/MIB.0000000000000691. View

Camelo A, Barlow J, Drynan L, Neill D, Ballantyne S, Wong S . Blocking IL-25 signalling protects against gut inflammation in a type-2 model of colitis by suppressing nuocyte and NKT derived IL-13. J Gastroenterol. 2012; 47(11):1198-211. PMC: 3501170. DOI: 10.1007/s00535-012-0591-2. View

Qiu W, Wu B, Wang X, Buchanan M, Regueiro M, Hartman D . PUMA-mediated intestinal epithelial apoptosis contributes to ulcerative colitis in humans and mice. J Clin Invest. 2011; 121(5):1722-32. PMC: 3083802. DOI: 10.1172/JCI42917. View

Lee J, Park E, Yuki Y, Ahmad S, Mizuguchi K, Ishii K . Profiles of microRNA networks in intestinal epithelial cells in a mouse model of colitis. Sci Rep. 2015; 5:18174. PMC: 4673535. DOI: 10.1038/srep18174. View

Fasseu M, Treton X, Guichard C, Pedruzzi E, Cazals-Hatem D, Richard C . Identification of restricted subsets of mature microRNA abnormally expressed in inactive colonic mucosa of patients with inflammatory bowel disease. PLoS One. 2010; 5(10). PMC: 2950152. DOI: 10.1371/journal.pone.0013160. View

Min M, Peng L, Yang Y, Guo M, Wang W, Sun G . MicroRNA-155 is involved in the pathogenesis of ulcerative colitis by targeting FOXO3a. Inflamm Bowel Dis. 2014; 20(4):652-9. DOI: 10.1097/MIB.0000000000000009. View

Su J, Chen T, Ji X, Liu C, Yadav P, Wu R . IL-25 downregulates Th1/Th17 immune response in an IL-10-dependent manner in inflammatory bowel disease. Inflamm Bowel Dis. 2013; 19(4):720-8. DOI: 10.1097/MIB.0b013e3182802a76. View

10.

McHenga S, Wang D, Li C, Shan F, Lu C . Inhibitory effect of recombinant IL-25 on the development of dextran sulfate sodium-induced experimental colitis in mice. Cell Mol Immunol. 2009; 5(6):425-31. PMC: 4072414. DOI: 10.1038/cmi.2008.53. View

11.

Garo L, Murugaiyan G . Contribution of MicroRNAs to autoimmune diseases. Cell Mol Life Sci. 2016; 73(10):2041-51. PMC: 11108434. DOI: 10.1007/s00018-016-2167-4. View

12.

Cohen R, Yu A, Wu E, Xie J, Mulani P, Chao J . Systematic review: the costs of ulcerative colitis in Western countries. Aliment Pharmacol Ther. 2010; 31(7):693-707. DOI: 10.1111/j.1365-2036.2010.04234.x. View

13.

Cai M, Chen S, Hu W . MicroRNA-141 Is Involved in Ulcerative Colitis Pathogenesis via Aiming at CXCL5. J Interferon Cytokine Res. 2017; 37(9):415-420. DOI: 10.1089/jir.2017.0019. View

14.

Zhao P, Zhao L, Zhang K, Feng H, Wang H, Wang T . Infection with street strain rabies virus induces modulation of the microRNA profile of the mouse brain. Virol J. 2012; 9:159. PMC: 3549733. DOI: 10.1186/1743-422X-9-159. View

15.

Takagi T, Naito Y, Mizushima K, Hirata I, Yagi N, Tomatsuri N . Increased expression of microRNA in the inflamed colonic mucosa of patients with active ulcerative colitis. J Gastroenterol Hepatol. 2010; 25 Suppl 1:S129-33. DOI: 10.1111/j.1440-1746.2009.06216.x. View

16.

Cheng C, Hua J, Tan J, Qian W, Zhang L, Hou X . Identification of differentially expressed genes, associated functional terms pathways, and candidate diagnostic biomarkers in inflammatory bowel diseases by bioinformatics analysis. Exp Ther Med. 2019; 18(1):278-288. PMC: 6566124. DOI: 10.3892/etm.2019.7541. View

17.

Loddo I, Romano C . Inflammatory Bowel Disease: Genetics, Epigenetics, and Pathogenesis. Front Immunol. 2015; 6:551. PMC: 4629465. DOI: 10.3389/fimmu.2015.00551. View

18.

Chen Q, Duan X, Fan H, Xu M, Tang Q, Zhang L . Oxymatrine protects against DSS-induced colitis via inhibiting the PI3K/AKT signaling pathway. Int Immunopharmacol. 2017; 53:149-157. DOI: 10.1016/j.intimp.2017.10.025. View

19.

Koukos G, Polytarchou C, Kaplan J, Oikonomopoulos A, Ziring D, Hommes D . A microRNA signature in pediatric ulcerative colitis: deregulation of the miR-4284/CXCL5 pathway in the intestinal epithelium. Inflamm Bowel Dis. 2015; 21(5):996-1005. PMC: 4402238. DOI: 10.1097/MIB.0000000000000339. View

20.

Li J, Liang H, Bai M, Ning T, Wang C, Fan Q . miR-135b Promotes Cancer Progression by Targeting Transforming Growth Factor Beta Receptor II (TGFBR2) in Colorectal Cancer. PLoS One. 2015; 10(6):e0130194. PMC: 4462589. DOI: 10.1371/journal.pone.0130194. View