Efficient 2,3-butanediol Production from Whey Powder Using Metabolically Engineered Klebsiella Oxytoca

Overview

Journal Microb Cell Fact

Publisher Biomed Central

Specialties Biotechnology
Microbiology

Date 2020 Aug 12

PMID 32778112

Citations 4

Authors

Wensi Meng

Yongjia Zhang

Menghao Cao

Wen Zhang

Chuanjuan Lu

Chunyu Yang

Chao Gao

Ping Xu

Cuiqing Ma

Affiliations

Soon will be listed here.

Abstract

Background: Whey is a major pollutant generated by the dairy industry. To decrease environmental pollution caused by the industrial release of whey, new prospects for its utilization need to be urgently explored. Here, we investigated the possibility of using whey powder to produce 2,3-butanediol (BDO), an important platform chemical.

Results: Klebsiella oxytoca strain PDL-0 was selected because of its ability to efficiently produce BDO from lactose, the major fermentable sugar in whey. After deleting genes pox, pta, frdA, ldhD, and pflB responding for the production of by-products acetate, succinate, lactate, and formate, a recombinant strain K. oxytoca PDL-K5 was constructed. Fed-batch fermentation using K. oxytoca PDL-K5 produced 74.9 g/L BDO with a productivity of 2.27 g/L/h and a yield of 0.43 g/g from lactose. In addition, when whey powder was used as the substrate, 65.5 g/L BDO was produced within 24 h with a productivity of 2.73 g/L/h and a yield of 0.44 g/g.

Conclusion: This study demonstrated the efficiency of K. oxytoca PDL-0 for BDO production from whey. Due to its non-pathogenicity and efficient lactose utilization, K. oxytoca PDL-0 might also be used in the production of other important chemicals using whey as the substrate.

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References

Li L, Li K, Wang Y, Chen C, Xu Y, Zhang L . Metabolic engineering of Enterobacter cloacae for high-yield production of enantiopure (2R,3R)-2,3-butanediol from lignocellulose-derived sugars. Metab Eng. 2014; 28:19-27. DOI: 10.1016/j.ymben.2014.11.010. View

Song C, Rathnasingh C, Park J, Lee J, Song H . Isolation and Evaluation of Strains for Industrial Production of 2,3-Butanediol. J Microbiol Biotechnol. 2017; 28(3):409-417. DOI: 10.4014/jmb.1710.10038. View

Prazeres A, Carvalho F, Rivas J . Cheese whey management: a review. J Environ Manage. 2012; 110:48-68. DOI: 10.1016/j.jenvman.2012.05.018. View

Heyman B, Tulke H, Putri S, Fukusaki E, Buchs J . Online monitoring of the respiratory quotient reveals metabolic phases during microaerobic 2,3-butanediol production with . Eng Life Sci. 2020; 20(3-4):133-144. PMC: 7447875. DOI: 10.1002/elsc.201900121. View

Asunis F, De Gioannis G, Isipato M, Muntoni A, Polettini A, Pomi R . Control of fermentation duration and pH to orient biochemicals and biofuels production from cheese whey. Bioresour Technol. 2019; 289:121722. DOI: 10.1016/j.biortech.2019.121722. View

Jantama K, Polyiam P, Khunnonkwao P, Chan S, Sangproo M, Khor K . Efficient reduction of the formation of by-products and improvement of production yield of 2,3-butanediol by a combined deletion of alcohol dehydrogenase, acetate kinase-phosphotransacetylase, and lactate dehydrogenase genes in metabolically.... Metab Eng. 2015; 30:16-26. DOI: 10.1016/j.ymben.2015.04.004. View

Song C, Park J, Chung S, Lee S, Song H . Microbial production of 2,3-butanediol for industrial applications. J Ind Microbiol Biotechnol. 2019; 46(11):1583-1601. DOI: 10.1007/s10295-019-02231-0. View

Farahnak F, Seki T, Ryu D, Ogrydziak D . Construction of lactose-assimilating and high-ethanol-producing yeasts by protoplast fusion. Appl Environ Microbiol. 1986; 51(2):362-7. PMC: 238874. DOI: 10.1128/aem.51.2.362-367.1986. View

Carvalho F, Prazeres A, Rivas J . Cheese whey wastewater: characterization and treatment. Sci Total Environ. 2013; 445-446:385-96. DOI: 10.1016/j.scitotenv.2012.12.038. View

10.

Saratale R, Shin H, Ghodake G, Kumar G, Oh M, Saratale G . Combined effect of inorganic salts with calcium peroxide pretreatment for kenaf core biomass and their utilization for 2,3-butanediol production. Bioresour Technol. 2018; 258:26-32. DOI: 10.1016/j.biortech.2018.02.115. View

11.

Jang J, Jung H, Im D, Jung M, Oh M . Pathway engineering of Enterobacter aerogenes to improve acetoin production by reducing by-products formation. Enzyme Microb Technol. 2017; 106:114-118. DOI: 10.1016/j.enzmictec.2017.07.009. View

12.

Moon S, Kim D, Park J, Min J, Song H . Development of a semi-continuous two-stage simultaneous saccharification and fermentation process for enhanced 2,3-butanediol production by Klebsiella oxytoca. Lett Appl Microbiol. 2018; 66(4):300-305. DOI: 10.1111/lam.12845. View

13.

Ma C, Wang A, Qin J, Li L, Ai X, Jiang T . Enhanced 2,3-butanediol production by Klebsiella pneumoniae SDM. Appl Microbiol Biotechnol. 2008; 82(1):49-57. DOI: 10.1007/s00253-008-1732-7. View

14.

Wang A, Xu Y, Ma C, Gao C, Li L, Wang Y . Efficient 2,3-butanediol production from cassava powder by a crop-biomass-utilizer, Enterobacter cloacae subsp. dissolvens SDM. PLoS One. 2012; 7(7):e40442. PMC: 3390385. DOI: 10.1371/journal.pone.0040442. View

15.

Guo X, Zhang Y, Cao C, Shen T, Wu M, Chen Y . Enhanced production of 2,3-butanediol by overexpressing acetolactate synthase and acetoin reductase in Klebsiella pneumoniae. Biotechnol Appl Biochem. 2014; 61(6):707-15. DOI: 10.1002/bab.1217. View

16.

Xin B, Tao F, Wang Y, Liu H, Ma C, Xu P . Coordination of metabolic pathways: Enhanced carbon conservation in 1,3-propanediol production by coupling with optically pure lactate biosynthesis. Metab Eng. 2017; 41:102-114. DOI: 10.1016/j.ymben.2017.03.009. View

17.

Rebecchi S, Pinelli D, Zanaroli G, Fava F, Frascari D . Effect of oxygen mass transfer rate on the production of 2,3-butanediol from glucose and agro-industrial byproducts by ATCC9789. Biotechnol Biofuels. 2018; 11:145. PMC: 5964669. DOI: 10.1186/s13068-018-1138-4. View

18.

Champluvier B, Francart B, Rouxhet P . Co-immobilization by adhesion of beta-galactosidase in nonviable cells of Kluyveromyces lactis with Klebsiella oxytoca: conversion of lactose into 2, 3-butanediol. Biotechnol Bioeng. 1989; 34(6):844-53. DOI: 10.1002/bit.260340614. View

19.

Cho S, Kim T, Woo H, Lee J, Kim Y, Um Y . Enhanced 2,3-Butanediol Production by Optimizing Fermentation Conditions and Engineering Klebsiella oxytoca M1 through Overexpression of Acetoin Reductase. PLoS One. 2015; 10(9):e0138109. PMC: 4569360. DOI: 10.1371/journal.pone.0138109. View

20.

Saratale G, Jung M, Oh M . Reutilization of green liquor chemicals for pretreatment of whole rice waste biomass and its application to 2,3-butanediol production. Bioresour Technol. 2016; 205:90-6. DOI: 10.1016/j.biortech.2016.01.028. View