» Articles » PMID: 32771978

Fine-Needle Aspiration-Based Patient-Derived Cancer Organoids

Abstract

Patient-derived cancer organoids hold great potential to accurately model and predict therapeutic responses. Efficient organoid isolation methods that minimize post-collection manipulation of tissues would improve adaptability, accuracy, and applicability to both experimental and real-time clinical settings. Here we present a simple and minimally invasive fine-needle aspiration (FNA)-based organoid culture technique using a variety of tumor types including gastrointestinal, thyroid, melanoma, and kidney. This method isolates organoids directly from patients at the bedside or from resected tissues, requiring minimal tissue processing while preserving the histologic growth patterns and infiltrating immune cells. Finally, we illustrate diverse downstream applications of this technique including in vitro high-throughput chemotherapeutic screens, in situ immune cell characterization, and in vivo patient-derived xenografts. Thus, routine clinical FNA-based collection techniques represent an unappreciated substantial source of material that can be exploited to generate tumor organoids from a variety of tumor types for both discovery and clinical applications.

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References
1.
Cabanillas M, Patel A, Danysh B, Dadu R, Kopetz S, Falchook G . BRAF inhibitors: experience in thyroid cancer and general review of toxicity. Horm Cancer. 2014; 6(1):21-36. PMC: 4312215. DOI: 10.1007/s12672-014-0207-9. View

2.
Jain D, Allen T, Aisner D, Beasley M, Cagle P, Capelozzi V . Rapid On-Site Evaluation of Endobronchial Ultrasound-Guided Transbronchial Needle Aspirations for the Diagnosis of Lung Cancer: A Perspective From Members of the Pulmonary Pathology Society. Arch Pathol Lab Med. 2017; 142(2):253-262. DOI: 10.5858/arpa.2017-0114-SA. View

3.
Boyd J, Smith G, Hong H, Mageau R, Juskevicius R . Fine-needle aspiration is superior to needle core biopsy as a sample acquisition method for flow cytometric analysis in suspected hematologic neoplasms. Cytometry B Clin Cytom. 2014; 88(1):64-8. DOI: 10.1002/cyto.b.21183. View

4.
Ernst L, Rimm D . Quantitative examination of mechanophysical tumor cell enrichment in fine-needle aspiration specimens. Cancer. 2002; 96(5):275-9. DOI: 10.1002/cncr.10746. View

5.
Tiriac H, Bucobo J, Tzimas D, Grewel S, LaComb J, Rowehl L . Successful creation of pancreatic cancer organoids by means of EUS-guided fine-needle biopsy sampling for personalized cancer treatment. Gastrointest Endosc. 2018; 87(6):1474-1480. PMC: 6143289. DOI: 10.1016/j.gie.2017.12.032. View