In Situ Hybridization of Nucleus Basalis Neurons Shows Increased Beta-amyloid MRNA in Alzheimer Disease

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1988 Feb 1

PMID 3277188

Citations 33

Authors

M L Cohen

T E Golde

M F Usiak

L H Younkin

S G Younkin

Affiliations

Soon will be listed here.

Abstract

To determine which cells within the brain produce beta-amyloid mRNA and to assess expression of the beta-amyloid gene in Alzheimer disease, we analyzed brain tissue from Alzheimer and control patients by in situ hybridization. Our results demonstrate that beta-amyloid mRNA is produced by neurons in the nucleus basalis of Meynert and cerebral cortex and that nucleus basalis perikarya from Alzheimer patients consistently hybridize more beta-amyloid probe than those from controls. These observations support the hypothesis that increased expression of the beta-amyloid gene plays an important role in the deposition of amyloid in the brains of patients with Alzheimer disease.

Citing Articles

Potential Implications of miRNAs in the Pathogenesis, Diagnosis, and Therapeutics of Alzheimer's Disease.

Wang L, Shui X, Diao Y, Chen D, Zhou Y, Lee T Int J Mol Sci. 2023; 24(22).

PMID: 38003448 PMC: 10671222. DOI: 10.3390/ijms242216259.

Transcriptional and Post-Transcriptional Regulations of Amyloid-β Precursor Protein APP mRNA.

Sato K, Takayama K, Hashimoto M, Inoue S Front Aging. 2022; 2:721579.

PMID: 35822056 PMC: 9261399. DOI: 10.3389/fragi.2021.721579.

Amyloid precursor protein glycosylation is altered in the brain of patients with Alzheimer's disease.

Boix C, Lopez-Font I, Cuchillo-Ibanez I, Saez-Valero J Alzheimers Res Ther. 2020; 12(1):96.

PMID: 32787955 PMC: 7425076. DOI: 10.1186/s13195-020-00664-9.

The amyloid precursor protein copper binding domain histidine residues 149 and 151 mediate APP stability and metabolism.

Spoerri L, Vella L, Pham C, Barnham K, Cappai R J Biol Chem. 2012; 287(32):26840-53.

PMID: 22685292 PMC: 3411021. DOI: 10.1074/jbc.M112.355743.

hnRNP C promotes APP translation by competing with FMRP for APP mRNA recruitment to P bodies.

Lee E, Kim H, Kuwano Y, Abdelmohsen K, Srikantan S, Subaran S Nat Struct Mol Biol. 2010; 17(6):732-9.

PMID: 20473314 PMC: 2908492. DOI: 10.1038/nsmb.1815.

References

Blessed G, Tomlinson B, Roth M . The association between quantitative measures of dementia and of senile change in the cerebral grey matter of elderly subjects. Br J Psychiatry. 1968; 114(512):797-811. DOI: 10.1192/bjp.114.512.797. View

Podlisny M, Lee G, Selkoe D . Gene dosage of the amyloid beta precursor protein in Alzheimer's disease. Science. 1987; 238(4827):669-71. DOI: 10.1126/science.2960019. View

Terry R, Peck A, DeTeresa R, Schechter R, Horoupian D . Some morphometric aspects of the brain in senile dementia of the Alzheimer type. Ann Neurol. 1981; 10(2):184-92. DOI: 10.1002/ana.410100209. View

Caruthers M, Beaucage S, Efcavitch J, FISHER E, Goldman R, deHaseth P . Chemical synthesis and biological studies on mutated gene-control regions. Cold Spring Harb Symp Quant Biol. 1983; 47 Pt 1:411-8. DOI: 10.1101/sqb.1983.047.01.048. View

Hall J, Dudley L, Dobner P, Lewis S, Cowan N . Identification of two human beta-tubulin isotypes. Mol Cell Biol. 1983; 3(5):854-62. PMC: 368608. DOI: 10.1128/mcb.3.5.854-862.1983. View

Cox K, DeLeon D, Angerer L, Angerer R . Detection of mrnas in sea urchin embryos by in situ hybridization using asymmetric RNA probes. Dev Biol. 1984; 101(2):485-502. DOI: 10.1016/0012-1606(84)90162-3. View

McGeer P, McGeer E, Suzuki J, Dolman C, Nagai T . Aging, Alzheimer's disease, and the cholinergic system of the basal forebrain. Neurology. 1984; 34(6):741-5. DOI: 10.1212/wnl.34.6.741. View

Glenner G, Wong C . Alzheimer's disease and Down's syndrome: sharing of a unique cerebrovascular amyloid fibril protein. Biochem Biophys Res Commun. 1984; 122(3):1131-5. DOI: 10.1016/0006-291x(84)91209-9. View

Lawrence J, Singer R . Quantitative analysis of in situ hybridization methods for the detection of actin gene expression. Nucleic Acids Res. 1985; 13(5):1777-99. PMC: 341111. DOI: 10.1093/nar/13.5.1777. View

10.

Masters C, Simms G, Weinman N, Multhaup G, McDonald B, Beyreuther K . Amyloid plaque core protein in Alzheimer disease and Down syndrome. Proc Natl Acad Sci U S A. 1985; 82(12):4245-9. PMC: 397973. DOI: 10.1073/pnas.82.12.4245. View

11.

Siegel R, Young 3rd W . Detection of preprocholecystokinin and preproenkephalin A mRNAs in rat brain by hybridization histochemistry using complementary RNA probes. Neuropeptides. 1985; 6(6):573-80. DOI: 10.1016/0143-4179(85)90121-0. View

12.

Lewis M, Sherman T, Watson S . In situ hybridization histochemistry with synthetic oligonucleotides: strategies and methods. Peptides. 1985; 6 Suppl 2:75-87. DOI: 10.1016/0196-9781(85)90138-x. View

13.

Katzman R . Alzheimer's disease. N Engl J Med. 1986; 314(15):964-73. DOI: 10.1056/NEJM198604103141506. View

14.

Younkin S, Goodridge B, Katz J, LOCKETT G, Nafziger D, Usiak M . Molecular forms of acetylcholinesterases in Alzheimer's disease. Fed Proc. 1986; 45(13):2982-8. View

15.

Mann D, YATES P, Marcyniuk B, Ravindra C . The topography of plaques and tangles in Down's syndrome patients of different ages. Neuropathol Appl Neurobiol. 1986; 12(5):447-57. DOI: 10.1111/j.1365-2990.1986.tb00053.x. View

16.

Robakis N, Wisniewski H, Jenkins E, Houck G, Yao X, Ramakrishna N . Chromosome 21q21 sublocalisation of gene encoding beta-amyloid peptide in cerebral vessels and neuritic (senile) plaques of people with Alzheimer disease and Down syndrome. Lancet. 1987; 1(8529):384-5. DOI: 10.1016/s0140-6736(87)91754-5. View

17.

Goldgaber D, Lerman M, MCBRIDE O, Saffiotti U, Gajdusek D . Characterization and chromosomal localization of a cDNA encoding brain amyloid of Alzheimer's disease. Science. 1987; 235(4791):877-80. DOI: 10.1126/science.3810169. View

18.

Tanzi R, Gusella J, Watkins P, BRUNS G, St George-Hyslop P, Van Keuren M . Amyloid beta protein gene: cDNA, mRNA distribution, and genetic linkage near the Alzheimer locus. Science. 1987; 235(4791):880-4. DOI: 10.1126/science.2949367. View

19.

Kang J, Lemaire H, Unterbeck A, Salbaum J, Masters C, Grzeschik K . The precursor of Alzheimer's disease amyloid A4 protein resembles a cell-surface receptor. Nature. 1987; 325(6106):733-6. DOI: 10.1038/325733a0. View

20.

Delabar J, Goldgaber D, Lamour Y, Nicole A, Huret J, de Grouchy J . Beta amyloid gene duplication in Alzheimer's disease and karyotypically normal Down syndrome. Science. 1987; 235(4794):1390-2. DOI: 10.1126/science.2950593. View