Evaluation of the Anti-Leishmanial Effect of Recombinant α-Toxin

Overview

Journal Infect Drug Resist

Publisher Dove Medical Press

Date 2020 Aug 9

PMID 32765010

Citations 1

Authors

Fatemeh Namdar

Hossein Khanahmad

Zahra Ghayour

Farzaneh Mirzaei

Azam Namdar

Maryam Aghaei

Shahrokh Izadi

Faham Khamesipour

Seyed Hossein Hejazi

Affiliations

Soon will be listed here.

Abstract

Background: Leishmaniasis is an infectious disease common in tropical and subtropical regions caused by the genus , which is transmitted by the bite of female sandflies. In this study, we evaluate the anti-leishmanial effect of recombinant α-toxin protein alone and the combination with glucantime through in vitro and in vivo.

Materials And Methods: Production, expression, and purification of recombinant α-toxin were evaluated by SDS-PAGE and Western blotting techniques. The antileishmanial activities of the purified α-toxin plus and without glucantime were examined in vitro and in vivo.

Results: The results indicated successful expression of α-toxin as a 48 kDa band on SDS-PAGE and Western blot methods. Also, evaluation of α-toxin IC showed the strong fatal effect of it, and glucantime on medium proliferated promastigotes at lower concentrations compared with glucantime or α-toxin alone. Moreover, in vivo surveys showed that at the end of treatment courses, the mean of lesion size diminished in glucantime plus α-toxin treated mice versus negative control groups (p < 0.001). Also, there was a significant difference in the parasite burden of the spleen and liver of the control versus the test groups (p < 0.001).

Conclusion: The results showed recombinant α-toxin has synergistic effects with glucantime in destroying parasites.

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