Anabolic-androgenic Steroids and Brain Injury: MiRNA Evaluation in Users Compared to Cocaine Abusers and Elderly People

Overview

Journal Aging (Albany NY)

Specialty Geriatrics

Date 2020 Aug 7

PMID 32756006

Citations 15

Authors

Francesco Sessa

Monica Salerno

Luigi Cipolloni

Giuseppe Bertozzi

Giovanni Messina

Giulio Di Mizio

Alessio Asmundo

Cristoforo Pomara

Affiliations

Soon will be listed here.

Abstract

Anabolic-androgenic steroids (AASs) can be used to treat both hormonal diseases and other pathologies characterized by muscle loss (aging, cancer, and AIDS). Even if the adverse effects related to the misuse of AASs have been well studied in different systems and apparatuses, knowledge about brain damage is poor.In this scenario, this experimental study aimed to analyze the role of several microRNAs (miRNAs) in brain damage after AAS misuse, to better comprehend the underlying mechanisms. The research hypothesis at the base of this experimental study is that the chronic use of AASs may be associated to brain damage with a dysregulation of these miRNAs. Moreover, miRNA expression values were compared among three different groups, "AAS" group, "Cocaine" group and "Aging" group, in order to define if AAS brain damage can be compared with the brain impairment linked to aging and/or cocaine assumption.This experimental study revealed that the tested miRNAs (hsa-miR-21-5p, hsa-miR-34a-5p, hsa-miR-124-5p, hsa-miR-132-3p, and hsa-miR-144-3p) were overexpressed in all enrolled groups. In the light of the presented results, the identification of specific circulating and/or tissue biomarkers is challenging for the scientific community. Further studies with larger samples are needed to confirm these interesting findings.

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References

Goldman A, Basaria S . Adverse health effects of androgen use. Mol Cell Endocrinol. 2017; 464:46-55. DOI: 10.1016/j.mce.2017.06.009. View

Sun Y, Luo Z, Guo X, Su D, Liu X . An updated role of microRNA-124 in central nervous system disorders: a review. Front Cell Neurosci. 2015; 9:193. PMC: 4438253. DOI: 10.3389/fncel.2015.00193. View

Favaloro B, Allocati N, Graziano V, Di Ilio C, De Laurenzi V . Role of apoptosis in disease. Aging (Albany NY). 2012; 4(5):330-49. PMC: 3384434. DOI: 10.18632/aging.100459. View

Pomara C, Barone R, Marino Gammazza A, Sangiorgi C, Barone F, Pitruzzella A . Effects of Nandrolone Stimulation on Testosterone Biosynthesis in Leydig Cells. J Cell Physiol. 2015; 231(6):1385-91. PMC: 5064776. DOI: 10.1002/jcp.25272. View

Fineschi V, DI Paolo M, Neri M, Bello S, DErrico S, Dinucci D . Anabolic steroid- and exercise-induced cardio-depressant cytokines and myocardial β1 receptor expression in CD1 mice. Curr Pharm Biotechnol. 2010; 12(2):275-84. DOI: 10.2174/138920111794295792. View

Rao Y, Mott N, Wang Y, Chung W, Pak T . MicroRNAs in the aging female brain: a putative mechanism for age-specific estrogen effects. Endocrinology. 2013; 154(8):2795-806. PMC: 3713211. DOI: 10.1210/en.2013-1230. View

Smith-Vikos T, Slack F . MicroRNAs and their roles in aging. J Cell Sci. 2012; 125(Pt 1):7-17. PMC: 3269020. DOI: 10.1242/jcs.099200. View

Hauger L, Westlye L, Fjell A, Walhovd K, Bjornebekk A . Structural brain characteristics of anabolic-androgenic steroid dependence in men. Addiction. 2019; 114(8):1405-1415. PMC: 6767448. DOI: 10.1111/add.14629. View

Sessa F, Salerno M, Di Mizio G, Bertozzi G, Messina G, Tomaiuolo B . Anabolic Androgenic Steroids: Searching New Molecular Biomarkers. Front Pharmacol. 2018; 9:1321. PMC: 6256094. DOI: 10.3389/fphar.2018.01321. View

10.

Fischbach S, Carew T . MicroRNAs in memory processing. Neuron. 2009; 63(6):714-6. DOI: 10.1016/j.neuron.2009.09.007. View

11.

Moya P, Wendland J, Salemme J, Fried R, Murphy D . miR-15a and miR-16 regulate serotonin transporter expression in human placental and rat brain raphe cells. Int J Neuropsychopharmacol. 2012; 16(3):621-9. DOI: 10.1017/S1461145712000454. View

12.

Ma K, Zhang H, Wei G, Dong Z, Zhao H, Han X . Identification of key genes, pathways, and miRNA/mRNA regulatory networks of CUMS-induced depression in nucleus accumbens by integrated bioinformatics analysis. Neuropsychiatr Dis Treat. 2019; 15:685-700. PMC: 6421879. DOI: 10.2147/NDT.S200264. View

13.

Bertozzi G, Salerno M, Pomara C, Sessa F . Neuropsychiatric and Behavioral Involvement in AAS Abusers. A Literature Review. Medicina (Kaunas). 2019; 55(7). PMC: 6681542. DOI: 10.3390/medicina55070396. View

14.

Monda V, Salerno M, Sessa F, Bernardini R, Valenzano A, Marsala G . Functional Changes of Orexinergic Reaction to Psychoactive Substances. Mol Neurobiol. 2018; 55(8):6362-6368. DOI: 10.1007/s12035-017-0865-z. View

15.

Kaufman M, Janes A, Hudson J, Brennan B, Kanayama G, Kerrigan A . Brain and cognition abnormalities in long-term anabolic-androgenic steroid users. Drug Alcohol Depend. 2015; 152:47-56. PMC: 4458166. DOI: 10.1016/j.drugalcdep.2015.04.023. View

16.

Hadar A, Milanesi E, Walczak M, Puzianowska-Kuznicka M, Kuznicki J, Squassina A . SIRT1, miR-132 and miR-212 link human longevity to Alzheimer's Disease. Sci Rep. 2018; 8(1):8465. PMC: 5981646. DOI: 10.1038/s41598-018-26547-6. View

17.

Dinan T . MicroRNAs as a target for novel antipsychotics: a systematic review of an emerging field. Int J Neuropsychopharmacol. 2009; 13(3):395-404. DOI: 10.1017/S1461145709990800. View

18.

Sagoe D, McVeigh J, Bjornebekk A, Essilfie M, Andreassen C, Pallesen S . Polypharmacy among anabolic-androgenic steroid users: a descriptive metasynthesis. Subst Abuse Treat Prev Policy. 2015; 10:12. PMC: 4377045. DOI: 10.1186/s13011-015-0006-5. View

19.

Lane R, Hilsabeck T, Rea S . The role of mitochondrial dysfunction in age-related diseases. Biochim Biophys Acta. 2015; 1847(11):1387-400. PMC: 10481969. DOI: 10.1016/j.bbabio.2015.05.021. View

20.

Murphy T, Dias G, Thuret S . Effects of diet on brain plasticity in animal and human studies: mind the gap. Neural Plast. 2014; 2014:563160. PMC: 4037119. DOI: 10.1155/2014/563160. View