Pseudonajide Peptide Derived from Snake Venom Alters Cell Envelope Integrity Interfering on Biofilm Formation in Staphylococcus Epidermidis

Overview

Journal BMC Microbiol

Publisher Biomed Central

Specialty Microbiology

Date 2020 Aug 5

PMID 32746783

Citations 4

Authors

Rafael Schneider

Muriel Primon-Barros

Rafael Gomes Von Borowski

Sophie Chat

Sylvie Nonin-Lecomte

Reynald Gillet

Alexandre Jose Macedo

Affiliations

Soon will be listed here.

Abstract

Background: The increase in bacterial resistance phenotype cases is a global health problem. New strategies must be explored by the scientific community in order to create new treatment alternatives. Animal venoms are a good source for antimicrobial peptides (AMPs), which are excellent candidates for new antimicrobial drug development. Cathelicidin-related antimicrobial peptides (CRAMPs) from snake venoms have been studied as a model for the design of new antimicrobial pharmaceuticals against bacterial infections.

Results: In this study we present an 11 amino acid-long peptide, named pseudonajide, which is derived from a Pseudonaja textilis venom peptide and has antimicrobial and antibiofilm activity against Staphylococcus epidermidis. Pseudonajide was selected based on the sequence alignments of various snake venom peptides that displayed activity against bacteria. Antibiofilm activity assays with pseudonajide concentrations ranging from 3.12 to 100 μM showed that the lowest concentration to inhibit biofilm formation was 25 μM. Microscopy analysis demonstrated that pseudonajide interacts with the bacterial cell envelope, disrupting the cell walls and membranes, leading to morphological defects in prokaryotes.

Conclusions: Our results suggest that pseudonajide's positives charges interact with negatively charged cell wall components of S. epidermidis, leading to cell damage and inhibiting biofilm formation.

Citing Articles

Cec4-Derived Peptide Inhibits Planktonic and Biofilm-Associated Methicillin Resistant Staphylococcus epidermidis.

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Disclosure of a Promising Lead to Tackle Complicated Skin and Skin Structure Infections: Antimicrobial and Antibiofilm Actions of Peptide PP4-3.1.

Gomes A, Bessa L, Fernandes I, Ferraz R, Monteiro C, Martins M Pharmaceutics. 2021; 13(11).

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Capsicumicine, a New Bioinspired Peptide from Red Peppers Prevents Staphylococcal Biofilm and via a Matrix Anti-Assembly Mechanism of Action.

Gomes Von Borowski R, Chat S, Schneider R, Nonin-Lecomte S, Bouaziz S, Giudice E Microbiol Spectr. 2021; 9(2):e0047121.

PMID: 34704807 PMC: 8549733. DOI: 10.1128/Spectrum.00471-21.

Combined Proteotranscriptomic-Based Strategy to Discover Novel Antimicrobial Peptides from Cone Snails.

Ebou A, Koua D, Addablah A, Kakou-Ngazoa S, Dutertre S Biomedicines. 2021; 9(4).

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