Pea Gβ Subunit of G Proteins Has a Role in Nitric Oxide-induced Stomatal Closure in Response to Heat and Drought Stress

Overview

Journal Protoplasma

Publisher Springer

Specialty Biology

Date 2020 Aug 2

PMID 32737572

Citations 5

Authors

Deepak Bhardwaj

Ranjan Kumar Sahoo

Afsar Raza Naqvi

Suman Lakhanpaul

Narendra Tuteja

Affiliations

Soon will be listed here.

Abstract

Heterotrimeric G proteins consisting of Gα, Gβ and Gγ subunits act as downstream effectors to regulate multiple functions including abiotic stress tolerance. However, the mechanism of Gβ-mediated heat and drought tolerance is yet to be established. To explore the role of Pisum sativum Gβ subunit (PsGβ) in heat and drought stress, transgenic tobacco plants overexpressing (OEs) PsGβ were raised. Transgenic plants showing ectopic expression of PsGβ performed better under heat and drought stress in comparison with vector control plants. The seed germination, relative water content (RWC) and nitric oxide (NO) induction in the guard cells of transgenic plants were significantly higher in contrast to control plants. PsGβ promoter was isolated and several stress-responsive elements were identified. The change in Gβ expression in response to heat, methyl jasmonate (MeJA), abscisic acid (ABA), drought and salt confirms the presence of heat, low temperature and drought-responsive elements in the PsGβ promoter. Also, heat and drought stress caused the release of NO-induced stomatal closure in the leaves of transgenic tobacco plants OEs PsGβ. The better performance of transgenic plant OEs PsGβ is also attributed to the improved photosynthetic parameters as compared with control plants. These findings suggest a role of PsGβ in the signalling pathway leading to NO-induced stomatal closure during heat and drought stress.

Citing Articles

An atypical heterotrimeric Gα and its interactome suggest an extra-large role in overcoming abiotic and biotic stress.

Sharma B, Ganotra J, Biswal B, Sharma K, Gandhi S, Bhardwaj D Physiol Mol Biol Plants. 2023; 29(10):1543-1561.

PMID: 38076761 PMC: 10709287. DOI: 10.1007/s12298-023-01378-6.

An emerging role of heterotrimeric G-proteins in nodulation and nitrogen sensing.

Sharma S, Ganotra J, Samantaray J, Sahoo R, Bhardwaj D, Tuteja N Planta. 2023; 258(5):101.

PMID: 37847414 DOI: 10.1007/s00425-023-04251-8.

G-Protein β-Subunit Gene Enhances Drought and Salt Resistance in Wheat.

Xiong X, Liu Y, Zhang L, Li X, Zhao Y, Zheng Y Int J Mol Sci. 2023; 24(8).

PMID: 37108500 PMC: 10138664. DOI: 10.3390/ijms24087337.

Elucidating the role of key physio-biochemical traits and molecular network conferring heat stress tolerance in cucumber.

Hongal D, Raju D, Kumar S, Talukdar A, Das A, Kumari K Front Plant Sci. 2023; 14:1128928.

PMID: 36895870 PMC: 9990136. DOI: 10.3389/fpls.2023.1128928.

Heterotrimeric G Protein Signaling in Abiotic Stress.

Wang Y, Botella J Plants (Basel). 2022; 11(7).

PMID: 35406855 PMC: 9002505. DOI: 10.3390/plants11070876.

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