Alpha 2.0
Login Register
» Articles » PMID: 32734277

Differentiation of Cardiomyocytes from Human Pluripotent Stem Cells in Fully Chemically Defined Conditions

Overview
Journal STAR Protoc
Publisher Cell Press
Specialties Biology
Biomedical Engineering
Science
Date 2020 Aug 1
PMID 32734277
Citations 19
Authors
Yongshun Lin
Jizhong Zou
Affiliations
Soon will be listed here.
Abstract

In the past few years, several different methods for differentiation of directed cardiomyocytes from human pluripotent stem cells (hPSCs) in chemically defined conditions have been reported, including our own (Burridge et al., 2014; Lian et al., 2012; Lin et al., 2017). To help researchers adapt to our simple and cost-effective method, we provide step-by-step protocols for hPSC-cardiomyocyte differentiation, including hPSC culture, cardiomyocyte differentiation, cardiomyocyte passaging, and cryopreservation. For complete details on the use and execution of this protocol, please refer to Lin et al. (2017).

Citing Articles

Applications, Limitations, and Considerations of Clinical Trials in a Dish.

Mir A, Zhu A, Lau R, Barr N, Sheikh Z, Acuna D Bioengineering (Basel). 2024; 11(11).

PMID: 39593756 PMC: 11591410. DOI: 10.3390/bioengineering11111096.

Gain of 1q confers an MDM4-driven growth advantage to undifferentiated and differentiating hESC while altering their differentiation capacity.

Krivec N, Couvreu de Deckersberg E, Lei Y, Al Delbany D, Regin M, Verhulst S Cell Death Dis. 2024; 15(11):852.

PMID: 39572522 PMC: 11582570. DOI: 10.1038/s41419-024-07236-x.

The Current State of Realistic Heart Models for Disease Modelling and Cardiotoxicity.

Kistamas K, Lamberto F, Vaiciuleviciute R, Leal F, Muenthaisong S, Marte L Int J Mol Sci. 2024; 25(17).

PMID: 39273136 PMC: 11394806. DOI: 10.3390/ijms25179186.

Optical redox imaging to screen synthetic hydrogels for stem cell-derived cardiomyocyte differentiation and maturation.

Desa D, Amitrano M, Murphy W, Skala M Biophotonics Discov. 2024; 1(1).

PMID: 39036366 PMC: 11258857. DOI: 10.1117/1.bios.1.1.015002.

Engineered heart tissue: Design considerations and the state of the art.

Gokhan I, Blum T, Campbell S Biophys Rev (Melville). 2024; 5(2):021308.

PMID: 38912258 PMC: 11192576. DOI: 10.1063/5.0202724.

References
1.
Melkoumian Z, Weber J, Weber D, Fadeev A, Zhou Y, Dolley-Sonneville P . Synthetic peptide-acrylate surfaces for long-term self-renewal and cardiomyocyte differentiation of human embryonic stem cells. Nat Biotechnol. 2010; 28(6):606-10. DOI: 10.1038/nbt.1629. View
2.
Burridge P, Matsa E, Shukla P, Lin Z, Churko J, Ebert A . Chemically defined generation of human cardiomyocytes. Nat Methods. 2014; 11(8):855-60. PMC: 4169698. DOI: 10.1038/nmeth.2999. View
3.
Robertson C, Tran D, George S . Concise review: maturation phases of human pluripotent stem cell-derived cardiomyocytes. Stem Cells. 2013; 31(5):829-37. PMC: 3749929. DOI: 10.1002/stem.1331. View
4.
Preissner K . Structure and biological role of vitronectin. Annu Rev Cell Biol. 1991; 7:275-310. DOI: 10.1146/annurev.cb.07.110191.001423. View
5.
Chen G, Gulbranson D, Hou Z, Bolin J, Ruotti V, Probasco M . Chemically defined conditions for human iPSC derivation and culture. Nat Methods. 2011; 8(5):424-9. PMC: 3084903. DOI: 10.1038/nmeth.1593. View