The Impact of Antioxidant Supplementation and Heat Stress on Carcass Characteristics, Muscle Nutritional Profile and Functionality of Lamb Meat

Overview

Journal Animals (Basel)

Date 2020 Aug 1

PMID 32731563

Citations 6

Authors

Surinder S Chauhan

Frank R Dunshea

Tim E Plozza

David L Hopkins

Eric N Ponnampalam

Affiliations

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Abstract

The impact of antioxidant supplementation and short-term heat stress on lamb body weight gain, meat nutritional profile and functionality (storage stability of lipids and colour) of lamb meat was investigated. A total of 48 crossbred ((Merino × Border Leicester) × Dorset) lambs (42 ± 2 kg body weight, 7 mo age) were randomly allocated to three dietary treatments ( = 16) by liveweight (LW) that differed in dosage of vitamin E and selenium (Se) in the diet. Vitamin E and Se levels in the control (CON), moderate (MOD) and supranutritional (SUP) dietary treatments were 28, 130 and 228 mg/kg DM as α-tocopherol acetate and 0.16, 0.66 and 1.16 mg Se as SelPlex™/kg DM, respectively. After four weeks of feeding in individual pens, including one week of adaptation, lambs were exposed to two heat treatments. Animals were moved to metabolism cages for one week and subjected to heat treatments: thermoneutral (TN; 18-21 °C and 40-50% relative humidity) and heat stress (HS; 28-40 °C and 30-40% relative humidity) conditions, respectively. Final LW and hot carcass weight were influenced by dietary treatments with higher final live weight (FLW) ( = 0.05; 46.8 vs. 44.4 and 43.8 kg, respectively) and hot carcass weight (HCW) ( = 0.01; 22.5 vs. 21.3 and 21.0 kg, respectively) recorded in lambs fed the SUP as opposed to the CON and MOD diets. Vitamin E concentration in the longissimus lumborum (LL) muscle tended to be higher in lambs fed MOD or SUP diets than the CON group. Lipid oxidation of aged meat at 72 h of simulated retail display was reduced by antioxidant supplementation. Short-term (one week) heat stress treatment significantly increased muscle linoleic acid and total omega-6 concentrations compared with the CON group. The results demonstrate that four-week antioxidant supplementation at the SUP level improved animal productivity by increasing LW and carcass weight and the functionality of meat exhibited by reduced lipid oxidation. An increase in muscle omega-6 fatty acid concentration from short-term heat stress may induce oxidative stress via proinflammatory action.

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References

Kasapidou E, Wood J, Richardson R, Sinclair L, Wilkinson R, Enser M . Effect of vitamin E supplementation and diet on fatty acid composition and on meat colour and lipid oxidation of lamb leg steaks displayed in modified atmosphere packs. Meat Sci. 2011; 90(4):908-16. DOI: 10.1016/j.meatsci.2011.11.031. View

Mishkind M, Vermeer J, Darwish E, Munnik T . Heat stress activates phospholipase D and triggers PIP accumulation at the plasma membrane and nucleus. Plant J. 2009; 60(1):10-21. DOI: 10.1111/j.1365-313X.2009.03933.x. View

Ponnampalam E, Butler K, McDonagh M, Jacobs J, Hopkins D . Relationship between muscle antioxidant status, forms of iron, polyunsaturated fatty acids and functionality (retail colour) of meat in lambs. Meat Sci. 2011; 90(2):297-303. DOI: 10.1016/j.meatsci.2011.07.014. View

Ponnampalam E, Plozza T, Kerr M, Linden N, Mitchell M, Bekhit A . Interaction of diet and long ageing period on lipid oxidation and colour stability of lamb meat. Meat Sci. 2017; 129:43-49. DOI: 10.1016/j.meatsci.2017.02.008. View

Simopoulos A . Evolutionary aspects of diet: the omega-6/omega-3 ratio and the brain. Mol Neurobiol. 2011; 44(2):203-15. DOI: 10.1007/s12035-010-8162-0. View

Ponnampalam E, Burnett V, Norng S, Hopkins D, Plozza T, Jacobs J . Muscle antioxidant (vitamin E) and major fatty acid groups, lipid oxidation and retail colour of meat from lambs fed a roughage based diet with flaxseed or algae. Meat Sci. 2015; 111:154-60. DOI: 10.1016/j.meatsci.2015.09.007. View

Fang Y, Yang S, Wu G . Free radicals, antioxidants, and nutrition. Nutrition. 2002; 18(10):872-9. DOI: 10.1016/s0899-9007(02)00916-4. View

Chauhan S, Celi P, Leury B, Dunshea F . High dietary selenium and vitamin E supplementation ameliorates the impacts of heat load on oxidative status and acid-base balance in sheep. J Anim Sci. 2015; 93(7):3342-54. DOI: 10.2527/jas.2014-8731. View

Bekhit A, Hopkins D, Fahri F, Ponnampalam E . Oxidative Processes in Muscle Systems and Fresh Meat: Sources, Markers, and Remedies. Compr Rev Food Sci Food Saf. 2021; 12(5):565-597. DOI: 10.1111/1541-4337.12027. View

10.

Suman S, Hunt M, Nair M, Rentfrow G . Improving beef color stability: practical strategies and underlying mechanisms. Meat Sci. 2014; 98(3):490-504. DOI: 10.1016/j.meatsci.2014.06.032. View

11.

Celi P, Gabai G . Oxidant/Antioxidant Balance in Animal Nutrition and Health: The Role of Protein Oxidation. Front Vet Sci. 2015; 2:48. PMC: 4672199. DOI: 10.3389/fvets.2015.00048. View

12.

Chauhan S, Celi P, Leury B, Clarke I, Dunshea F . Dietary antioxidants at supranutritional doses improve oxidative status and reduce the negative effects of heat stress in sheep. J Anim Sci. 2014; 92(8):3364-74. DOI: 10.2527/jas.2014-7714. View

13.

Aengwanich W, Kongbuntad W, Boonsorn T . Effects of shade on physiological changes, oxidative stress, and total antioxidant power in Thai Brahman cattle. Int J Biometeorol. 2010; 55(5):741-8. DOI: 10.1007/s00484-010-0389-y. View

14.

Ponnampalam E, Norng S, Burnett V, Dunshea F, Jacobs J, Hopkins D . The synergism of biochemical components controlling lipid oxidation in lamb muscle. Lipids. 2014; 49(8):757-66. DOI: 10.1007/s11745-014-3916-5. View

15.

Ponnampalam E, Sinclair A, Egan A, Blakeley S, Li D, Leury B . Effect of dietary modification of muscle long-chain n-3 fatty acid on plasma insulin and lipid metabolites, carcass traits, and fat deposition in lambs. J Anim Sci. 2001; 79(4):895-903. DOI: 10.2527/2001.794895x. View

16.

Khliji S, van de Ven R, Lamb T, Lanza M, Hopkins D . Relationship between consumer ranking of lamb colour and objective measures of colour. Meat Sci. 2010; 85(2):224-9. DOI: 10.1016/j.meatsci.2010.01.002. View

17.

Lobo V, Patil A, Phatak A, Chandra N . Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev. 2012; 4(8):118-26. PMC: 3249911. DOI: 10.4103/0973-7847.70902. View