De Novo Transcriptome Assembly and Gene Expression Profiling of the Copepod Feeding on the PUA-Producing Diatom

Overview

Journal Mar Drugs

Publisher MDPI

Specialties Biology
Pharmacology

Date 2020 Jul 31

PMID 32727111

Citations 12

Authors

Sneha Asai

Remo Sanges

Chiara Lauritano

Penelope K Lindeque

Francesco Esposito

Adrianna Ianora

Ylenia Carotenuto

Affiliations

Soon will be listed here.

Abstract

Diatoms are the dominant component of the marine phytoplankton. Several diatoms produce secondary metabolites, namely oxylipins, with teratogenic effects on their main predators, crustacean copepods. Our study reports the de novo assembled transcriptome of the calanoid copepod feeding on the oxylipin-producing diatom . Differential expression analysis was also performed between copepod females exposed to the diatom and the control flagellate , which does not produce oxylipins. Our results showed that transcripts involved in carbohydrate, amino acid, folate and methionine metabolism, embryogenesis, and response to stimulus were differentially expressed in the two conditions. Expression of 27 selected genes belonging to these functional categories was also analyzed by RT-qPCR in females exposed to a mixed solution of the oxylipins heptadienal and octadienal at the concentration of 10 µM, 15 µM, and 20 µM. The results confirmed differential expression analysis, with up-regulation of genes involved in stress response and down-regulation of genes associated with folate and methionine metabolism, embryogenesis, and signaling. Overall, we offer new insights on the mechanism of action of oxylipins on maternally-induced embryo abnormality. Our results may also help identify biomarker genes associated with diatom-related reproductive failure in the natural copepod population at sea.

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