MiR-494 Promotes Progression of Retinoblastoma Via PTEN Through PI3K/AKT Signaling Pathway

Overview

Journal Oncol Lett

Specialty Oncology

Date 2020 Jul 30

PMID 32724440

Citations 8

Authors

Fen Xu

Guiqin Liu

Lijuan Wang

Xiyan Wang

Xiao Jin

Wen Bo

Affiliations

Soon will be listed here.

Abstract

Increasing evidence has indicated that the dysregulation of microRNA (miRNA) occur in the pathogenesis of retinoblastoma (RB). Aim of the present study was to investigate the possible role of miR-494 (miR-494-3p) in RB. It was demonstrated that miR-494 expression was increased in RB tissue samples and cell lines. Also, it was prominently associated with clinicopathological features. Functional assays showed that RB cell proliferation, invasion and migration can be promoted by miR-494 overexpression. Besides, phosphatase and tensin homolog (PTEN) was verified as a possible target of miR-494 by a luciferase assay, western blot and qRT-PCR assay in RB. miR-494 and PTEN expression was negatively related in a correlation analysis on tumor tissues of 66 patients. In addition, PTEN was proved to reverse miR-494 effect on RB cell progression. Moreover, PI3K/AKT signaling pathway was validated to take part in RB progression. Taken together, the current study proposes that miR-494 might function as a tumor promoter and regulates RB progression through targeting PTEN.

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References

Zhao Y, Yang W, Xin H, Han J, Ma S . MiR-182-5p Knockdown Targeting PTEN Inhibits Cell Proliferation and Invasion of Breast Cancer Cells. Yonsei Med J. 2019; 60(2):148-157. PMC: 6342713. DOI: 10.3349/ymj.2019.60.2.148. View

Xu W, Yang Z, Zhou S, Lu N . Posttranslational regulation of phosphatase and tensin homolog (PTEN) and its functional impact on cancer behaviors. Drug Des Devel Ther. 2014; 8:1745-51. PMC: 4199979. DOI: 10.2147/DDDT.S71061. View

He H, Liao X, Yang Q, Liu Y, Peng Y, Zhong H . MicroRNA-494-3p Promotes Cell Growth, Migration, and Invasion of Nasopharyngeal Carcinoma by Targeting Sox7. Technol Cancer Res Treat. 2018; 17:1533033818809993. PMC: 6259066. DOI: 10.1177/1533033818809993. View

Shen Z, Li Y, Zhao C, Wang F, Zhou R, Chen G . miR‑494‑BAG‑1 axis is involved in cinobufacini‑induced cell proliferation and apoptosis in gastric cancer. Mol Med Rep. 2018; 17(5):7435-7441. DOI: 10.3892/mmr.2018.8788. View

Liu S, Hu C, Wang Y, Shi G, Li Y, Wu H . miR-124 inhibits proliferation and invasion of human retinoblastoma cells by targeting STAT3. Oncol Rep. 2016; 36(4):2398-404. DOI: 10.3892/or.2016.4999. View

Spangle J, Roberts T, Zhao J . The emerging role of PI3K/AKT-mediated epigenetic regulation in cancer. Biochim Biophys Acta Rev Cancer. 2017; 1868(1):123-131. PMC: 5548615. DOI: 10.1016/j.bbcan.2017.03.002. View

Schroeder C, Grell J, Hube-Magg C, Kluth M, Lang D, Simon R . Aberrant expression of the microtubule-associated protein tau is an independent prognostic feature in prostate cancer. BMC Cancer. 2019; 19(1):193. PMC: 6397474. DOI: 10.1186/s12885-019-5390-1. View

Xie C, Lu H, Nomura A, Hanse E, Forster C, Parker J . Co-deleting Pten with Rb in retinal progenitor cells in mice results in fully penetrant bilateral retinoblastomas. Mol Cancer. 2015; 14:93. PMC: 4411757. DOI: 10.1186/s12943-015-0360-y. View

Zhang Y, Guo L, Li Y, Feng G, Teng F, Li W . MicroRNA-494 promotes cancer progression and targets adenomatous polyposis coli in colorectal cancer. Mol Cancer. 2018; 17(1):1. PMC: 5755155. DOI: 10.1186/s12943-017-0753-1. View

10.

Wang Z, Lu B, Wang H, Cheng Z, Yin Y . MicroRNA-21 modulates chemosensitivity of breast cancer cells to doxorubicin by targeting PTEN. Arch Med Res. 2011; 42(4):281-90. DOI: 10.1016/j.arcmed.2011.06.008. View

11.

Fu X, Wen H, Jing L, Yang Y, Wang W, Liang X . MicroRNA-155-5p promotes hepatocellular carcinoma progression by suppressing PTEN through the PI3K/Akt pathway. Cancer Sci. 2017; 108(4):620-631. PMC: 5406601. DOI: 10.1111/cas.13177. View

12.

Tay Y, Kats L, Salmena L, Weiss D, Tan S, Ala U . Coding-independent regulation of the tumor suppressor PTEN by competing endogenous mRNAs. Cell. 2011; 147(2):344-57. PMC: 3235920. DOI: 10.1016/j.cell.2011.09.029. View

13.

Kim T, Park J, Woo J . Resveratrol induces cell death through ROS‑dependent downregulation of Notch1/PTEN/Akt signaling in ovarian cancer cells. Mol Med Rep. 2019; 19(4):3353-3360. DOI: 10.3892/mmr.2019.9962. View

14.

Dalgard C, Gonzalez M, deNiro J, OBrien J . Differential microRNA-34a expression and tumor suppressor function in retinoblastoma cells. Invest Ophthalmol Vis Sci. 2009; 50(10):4542-51. DOI: 10.1167/iovs.09-3520. View

15.

Li J, Yen C, Liaw D, Podsypanina K, Bose S, Wang S . PTEN, a putative protein tyrosine phosphatase gene mutated in human brain, breast, and prostate cancer. Science. 1997; 275(5308):1943-7. DOI: 10.1126/science.275.5308.1943. View

16.

Yu F, Pang G, Zhao G . RETRACTED: ANRIL acts as onco-lncRNA by regulation of microRNA-24/c-Myc, MEK/ERK and Wnt/β-catenin pathway in retinoblastoma. Int J Biol Macromol. 2019; 128:583-592. DOI: 10.1016/j.ijbiomac.2019.01.157. View

17.

Guo L, Bai Y, Ji S, Ma H . MicroRNA‑98 suppresses cell growth and invasion of retinoblastoma via targeting the IGF1R/k‑Ras/Raf/MEK/ERK signaling pathway. Int J Oncol. 2019; 54(3):807-820. PMC: 6365030. DOI: 10.3892/ijo.2019.4689. View

18.

Wei D, Miao Y, Yu L, Wang D, Wang Y . Downregulation of microRNA‑198 suppresses cell proliferation and invasion in retinoblastoma by directly targeting PTEN. Mol Med Rep. 2018; 18(1):595-602. DOI: 10.3892/mmr.2018.8979. View

19.

Wang J, Wang X, Li Z, Liu H, Teng Y . MicroRNA-183 suppresses retinoblastoma cell growth, invasion and migration by targeting LRP6. FEBS J. 2013; 281(5):1355-1365. DOI: 10.1111/febs.12659. View

20.

Sun Z, Zhang A, Zhang L . Inhibition of microRNA‑492 attenuates cell proliferation and invasion in retinoblastoma via directly targeting LATS2. Mol Med Rep. 2018; 19(3):1965-1971. DOI: 10.3892/mmr.2018.9784. View