Metabolic Profiling Reveals Significant Perturbations of Intracellular Glucose Homeostasis in -Infected Cells

Overview

Journal Metabolites

Publisher MDPI

Date 2020 Jul 29

PMID 32717953

Citations 8

Authors

Zijiao Zou

Jessica Oi-Ling Tsang

Bingpeng Yan

Kenn Ka-Heng Chik

Chris Chun-Yiu Chan

Jianli Cao

Ronghui Liang

Kaiming Tang

Feifei Yin

Zi-Wei Ye

Hin Chu

Jasper Fuk-Woo Chan

Shuofeng Yuan

Kwok-Yung Yuen

Affiliations

Soon will be listed here.

Abstract

A71 (EV-A71) is a common cause of hand, foot, and mouth disease. Severe EV-A71 infections may be associated with life-threatening neurological complications. However, the pathogenic mechanisms underlying these severe clinical and pathological features remain incompletely understood. Metabolites are known to play critical roles in multiple stages of the replication cycles of viruses. The metabolic reprogramming induced by viral infections is essential for optimal virus replication and may be potential antiviral targets. In this study, we applied targeted metabolomics profiling to investigate the metabolic changes of induced pluripotent human stem cell (iPSC)-derived neural progenitor cells (NPCs) upon EV-A71 infection. A targeted quantitation of polar metabolites identified 14 candidates with altered expression profiles. A pathway enrichment analysis pinpointed glucose metabolic pathways as being highly perturbed upon EV-A71 infection. Gene silencing of one of the key enzymes of glycolysis, 6-phosphofructo-2-kinase (PFKFB3), significantly suppressed EV-A71 replication in vitro. Collectively, we demonstrated the feasibility to manipulate EV-A71-triggered host metabolic reprogramming as a potential anti-EV-A71 strategy.

Citing Articles

Metabolomic analysis reveals an important role of sphingosine 1-phosphate in the development of HFMD due to EV-A71 infection.

Ji W, Dang D, Zhou G, Tao L, Sun T, Li D Antimicrob Agents Chemother. 2024; 69(2):e0127224.

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PMI-controlled mannose metabolism and glycosylation determines tissue tolerance and virus fitness.

Liang R, Ye Z, Qin Z, Xie Y, Yang X, Sun H Nat Commun. 2024; 15(1):2144.

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Modulation of nucleotide metabolism by picornaviruses.

Nouwen L, Breeuwsma M, Zaal E, van de Lest C, Buitendijk I, Zwaagstra M PLoS Pathog. 2024; 20(3):e1012036.

PMID: 38457376 PMC: 10923435. DOI: 10.1371/journal.ppat.1012036.

Transcriptome profiling of A549 non-small cell lung cancer cells in response to muscle larvae excretory/secretory products.

Wang H, Zhu Y, Li M, Pan J, Li D, Guo W Front Vet Sci. 2023; 10:1208538.

PMID: 37601754 PMC: 10433203. DOI: 10.3389/fvets.2023.1208538.

Metabolomics meets systems immunology.

Fu J, Zhu F, Xu C, Li Y EMBO Rep. 2023; 24(4):e55747.

PMID: 36916532 PMC: 10074123. DOI: 10.15252/embr.202255747.

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