Multigene Engineering of Medium-chain Fatty Acid Biosynthesis in Transgenic by a Multigene Expression System

Overview

Journal 3 Biotech

Publisher Springer

Specialty Biotechnology

Date 2020 Jul 28

PMID 32714735

Authors

Yusheng Zheng

Lizhi Chen

Zhiyong Zhu

Dongdong Li

Peng Zhou

Affiliations

Soon will be listed here.

Abstract

Medium-chain fatty acids (MCFAs) are an ideal feedstock for biodiesel and a range of oleochemical products. In this study, different combinations of , and from coconut ( L.) were coexpressed in transgenic a Cre/LoxP multigene expression system. Transgenic lines expressing different combinations of these genes were designated FL ( + ), FK ( + ) and FLK ( + + ). The homozygous seeds of transgenic expressing high levels of these genes were screened, and their fatty acid composition and lipid contents were determined. Compared with its content in wild-type , the lauric acid (C12:0) content was significantly increased by at least 395%, 134% and 124% in FLK, FL and FK seeds, respectively. Meanwhile, the myristic acid (C14:0) content was significantly increased by at least 383%, 106% and 102% in FL, FLK and FK seeds, respectively, compared to its level in wild-type seeds. Therefore, the FLK plants exhibited the best effects to increase the level of C12:0, and FL expressed the optimal combination of genes to increase the level of 14:0 MCFA.

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