A Simple GC-MS/MS Method for Determination of Smoke Taint-Related Volatile Phenols in Grapes

Overview

Journal Metabolites

Publisher MDPI

Date 2020 Jul 26

PMID 32709091

Citations 10

Authors

Zhiqian Liu

Vilnis Ezernieks

Priyanka Reddy

Aaron Elkins

Christian Krill

Kieran Murphy

Simone Rochfort

German Spangenberg

Affiliations

Soon will be listed here.

Abstract

Volatile phenols (VPs) derived from smoke-exposed grapes are known to confer a smoky flavor to wine. Current methods for determination of VPs in grape berries either involve complex sample purification/derivatization steps or employ two analytical platforms for free and bound VP fractions. We report here a simple gas chromatography-tandem mass spectrometry (GC-MS/MS) method for quantification of both free and bound VPs in grapes, based on optimized (1) GC-MS/MS parameters, (2) an analyte extraction procedure, and (3) phenol glycoside hydrolysis conditions. Requiring neither sample cleanup nor a derivatization step, this method is sensitive (LOD ≤ 1 ng/g berries) and reproducible (RSD < 12% for repeated analyses) and is expected to significantly reduce the sample turnover time for smoke taint detection in vineyards.

Citing Articles

Transcriptome analysis reveals activation of detoxification and defense mechanisms in smoke-exposed Merlot grape (Vitis vinifera) berries.

Hewitt S, Aragon M, Ashmore P, Collins T, Dhingra A Sci Rep. 2024; 14(1):21330.

PMID: 39266584 PMC: 11393342. DOI: 10.1038/s41598-024-72079-7.

Detection of biomarkers for characterizing smoke-taint in grapes.

Rochfort S, Reddy P, Fernanado K, Liu Z, Ezernieks V, Spangenberg G Food Chem X. 2024; 23:101665.

PMID: 39257488 PMC: 11385792. DOI: 10.1016/j.fochx.2024.101665.

Discovery of Potent Glycosidases Enables Quantification of Smoke-Derived Phenolic Glycosides through Enzymatic Hydrolysis.

Cui Y, Riley M, Moreno M, Cepeda M, Perez I, Wen Y J Agric Food Chem. 2024; 72(20):11617-11628.

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Gel polymer electrolyte-based dual screen-printed electrodes for the headspace quantification of 4-ethylphenol and ethanethiol simultaneously in wines.

Portugal-Gomez P, Dominguez-Renedo O, Alonso-Lomillo M Mikrochim Acta. 2024; 191(4):208.

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Casey J, Jackson S, Ryan J, Newton S J Chromatogr A. 2023; 1693:463884.

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