Serosurvey and Molecular Detection of the Main Zoonotic Parasites Carried by Commensal Population in Tehran, Iran

Overview

Journal Trop Med Health

Specialty Tropical Medicine

Date 2020 Jul 25

PMID 32704233

Citations 7

Authors

Taher Azimi

Mohammad Reza Pourmand

Fatemeh Fallah

Abdollah Karimi

Roxana Mansour-Ghanaie

Seyedeh Mahsan Hoseini-Alfatemi

Mehdi Shirdoust

Leila Azimi

Affiliations

Soon will be listed here.

Abstract

Background: are reservoirs for transmission of various zoonotic parasites, and they have become a threat to public health worldwide. Given the large number and the significant presence of throughout the city of Tehran, this study aims to assess the frequency of zoonotic parasites carried by commensal rodents wandering in Tehran, Iran. The study considered the north, south, west, east, and center regions of Tehran for the purposes of this study. The serological tests were applied in order to detect effective antibodies against (), spp., and spp. using a commercial qualitative rat ELISA kit. The frequency of () was surveyed by using the conventional PCR method. Furthermore, nested PCR was employed to detect the presence of spp. and spp. in commensal dispersed in Tehran.

Results: Approximately, 76% of the 100 tested were infected with at least one zoonotic parasite, indicating the significant frequency of parasites within the study areas. Seroreactivity against , spp., and spp. was detected in 5%, 0%, and 1% of the tested, respectively. DNA was detected in 32 out of 100 (32%) . In addition, spp. and spp. DNA were found in 18 out of 100 (18%) and 76 out of 100 (76%) investigated, respectively. with 15% and with 70% had the highest frequency of parasites among the collected from the western and northeastern regions of Tehran, respectively. Moreover, spp. with 95% and spp. with 30% had the highest frequency in the east and center districts, respectively.

Conclusion: The findings showed a wide geographical dissemination of spp., , and spp. in within five districts of Tehran. In contrast, other parasites such as spp. infection were rarely detected in populations. No evidence for the circulation of spp. was found in this study.

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References

Paparini A, Jackson B, Ward S, Young S, Ryan U . Multiple Cryptosporidium genotypes detected in wild black rats (Rattus rattus) from northern Australia. Exp Parasitol. 2012; 131(4):404-12. DOI: 10.1016/j.exppara.2012.05.009. View

Maas M, de Vries A, Reusken C, Buijs J, Goris M, Hartskeerl R . Prevalence of spp. and Seoul hantavirus in brown rats () in four regions in the Netherlands, 2011-2015. Infect Ecol Epidemiol. 2018; 8(1):1490135. PMC: 6022222. DOI: 10.1080/20008686.2018.1490135. View

Akhoundi M, Mohebali M, Asadi M, Mahmodi M, Amraei K, Mirzaei A . Molecular characterization of Leishmania spp. in reservoir hosts in endemic foci of zoonotic cutaneous leishmaniasis in Iran. Folia Parasitol (Praha). 2013; 60(3):218-24. DOI: 10.14411/fp.2013.024. View

Pellizzaro M, de Oliveira Conrado F, Martins C, Joaquim S, Ferreira F, Langoni H . Serosurvey of Leptospira spp. and Toxoplasma gondii in rats captured from two zoos in Southern Brazil. Rev Soc Bras Med Trop. 2018; 50(6):857-860. DOI: 10.1590/0037-8682-0138-2017. View

Pereira A, Ferreira E, Lima A, Tonelli G, Rego F, Paglia A . Detection of Leishmania spp in silvatic mammals and isolation of Leishmania (Viannia) braziliensis from Rattus rattus in an endemic area for leishmaniasis in Minas Gerais State, Brazil. PLoS One. 2017; 12(11):e0187704. PMC: 5703529. DOI: 10.1371/journal.pone.0187704. View

Eder M, Cortes F, de Siqueira Filha N, Araujo de Franca G, Degroote S, Braga C . Scoping review on vector-borne diseases in urban areas: transmission dynamics, vectorial capacity and co-infection. Infect Dis Poverty. 2018; 7(1):90. PMC: 6120094. DOI: 10.1186/s40249-018-0475-7. View

Navea-Perez H, Diaz-Saez V, Corpas-Lopez V, Merino-Espinosa G, Morillas-Marquez F, Martin-Sanchez J . Leishmania infantum in wild rodents: reservoirs or just irrelevant incidental hosts?. Parasitol Res. 2015; 114(6):2363-70. DOI: 10.1007/s00436-015-4434-y. View

Meerburg B, Singleton G, Kijlstra A . Rodent-borne diseases and their risks for public health. Crit Rev Microbiol. 2009; 35(3):221-70. DOI: 10.1080/10408410902989837. View

Galan-Puchades M, Sanxis-Furio J, Pascual J, Bueno-Mari R, Franco S, Peracho V . First survey on zoonotic helminthosis in urban brown rats (Rattus norvegicus) in Spain and associated public health considerations. Vet Parasitol. 2018; 259:49-52. DOI: 10.1016/j.vetpar.2018.06.023. View

10.

Marcelino A, Ferreira E, Avendanha J, Costa C, Chiarelli D, Almeida G . Molecular detection of Leishmania braziliensis in Rattus norvegicus in an area endemic for cutaneous leishmaniasis in Brazil. Vet Parasitol. 2011; 183(1-2):54-8. DOI: 10.1016/j.vetpar.2011.06.019. View

11.

Dellarupe A, Fitte B, Pardini L, Campero L, Bernstein M, Robles M . Toxoplasma gondii and Neospora caninum infections in synanthropic rodents from Argentina. Rev Bras Parasitol Vet. 2019; 28(1):113-118. DOI: 10.1590/S1984-29612019009. View

12.

Azimi T, Nasiri M, Zamani S, Hashemi A, Goudarzi H, Fooladi A . High genetic diversity among strains in Tehran, Iran. J Clin Tuberc Other Mycobact Dis. 2019; 11:1-6. PMC: 6830142. DOI: 10.1016/j.jctube.2018.01.001. View

13.

Salotra P, Sreenivas G, Pogue G, Lee N, Nakhasi H, Ramesh V . Development of a species-specific PCR assay for detection of Leishmania donovani in clinical samples from patients with kala-azar and post-kala-azar dermal leishmaniasis. J Clin Microbiol. 2001; 39(3):849-54. PMC: 87840. DOI: 10.1128/JCM.39.3.849-854.2001. View

14.

Yin C, He Y, Zhou D, Yan C, He X, Wu S . Seroprevalence of Toxoplasma gondii in rats in southern China. J Parasitol. 2010; 96(6):1233-4. DOI: 10.1645/GE-2610.1. View

15.

Bajer A . Between-year variation and spatial dynamics of Cryptosporidium spp. and Giardia spp. infections in naturally infected rodent populations. Parasitology. 2008; 135(14):1629-49. DOI: 10.1017/S0031182008004952. View

16.

Kimura A, Edagawa A, Okada K, Takimoto A, Yonesho S, Karanis P . Detection and genotyping of Cryptosporidium from brown rats (Rattus norvegicus) captured in an urban area of Japan. Parasitol Res. 2007; 100(6):1417-20. DOI: 10.1007/s00436-007-0488-9. View

17.

Sharifipour S, Davoodi Rad K . Seroprevalence of hepatitis E virus among different age groups in Tehran, Iran. New Microbes New Infect. 2020; 34:100638. PMC: 6976942. DOI: 10.1016/j.nmni.2019.100638. View

18.

Araujo J, da Silva A, Rosa R, Mattei R, da Silva R, Richini-Pereira V . Isolation and multilocus genotyping of Toxoplasma gondii in seronegative rodents in Brazil. Vet Parasitol. 2010; 174(3-4):328-31. DOI: 10.1016/j.vetpar.2010.08.039. View

19.

Saki J, Khademvatan S . Detection of Toxoplasma gondii by PCR and mouse bioassay in rodents of Ahvaz district, southwestern Iran. Biomed Res Int. 2014; 2014:383859. PMC: 3925531. DOI: 10.1155/2014/383859. View

20.

Ng-Hublin J, Singleton G, Ryan U . Molecular characterization of Cryptosporidium spp. from wild rats and mice from rural communities in the Philippines. Infect Genet Evol. 2013; 16:5-12. DOI: 10.1016/j.meegid.2013.01.011. View