Human Follicular Fluid Shows Diverse Metabolic Profiles at Different Follicle Developmental Stages

Overview

Journal Reprod Biol Endocrinol

Publisher Biomed Central

Specialties Endocrinology
Reproductive Medicine

Date 2020 Jul 25

PMID 32703275

Citations 15

Authors

Jihong Yang

Ting Feng

Suying Li

Xinyue Zhang

Yun Qian

Affiliations

Soon will be listed here.

Abstract

Background: To investigate the metabolic profiles in the follicular fluid (FF) samples from patients undergoing in vitro fertilization (IVF) and to analyze the correlations with follicular development.

Methods: The FF samples were obtained from participants (N = 26) who were receiving IVF under the gonadotropin-releasing hormone agonist (GnRH-a) long protocol stimulation and were collected separately from small (8-13 mm) and large (17-22 mm) follicles at the time of oocyte retrieval. Metabolomic analysis of the FF samples was performed using liquid chromatography-tandem mass spectrometry (LC-MS/MS).

Results: The results demonstrated that the size of the follicle influences the metabolic signature of the FF according to the profile and differential metabolites. Dehydroepiandrosterone (DHEA), which is enriched in steroid hormone biosynthesis, correlated negatively with the oocyte maturation rate and the high-quality embryo rate, and thus could be used to estimate the predictive diagnostic potential of follicular development.

Conclusion: The FF has different metabolic characteristics in different stages of follicular development. Exploring meaningful metabolites could predict follicular development, and modifications of these metabolites could influence follicular development.

Citing Articles

Unraveling the complexity of follicular fluid: insights into its composition, function, and clinical implications.

Pan Y, Pan C, Zhang C J Ovarian Res. 2024; 17(1):237.

PMID: 39593094 PMC: 11590415. DOI: 10.1186/s13048-024-01551-9.

Transplantation of human umbilical cord-derived mesenchymal stem cells improves age-related ovarian functional decline via regulating the local renin-angiotensin system on inflammation and oxidative stress.

Wei L, Bo L, Luo C, Yin N, Jiang W, Qian F Stem Cell Res Ther. 2024; 15(1):377.

PMID: 39444026 PMC: 11515572. DOI: 10.1186/s13287-024-03997-6.

Follicular Fluid Metabolomics: Tool for Predicting IVF Outcomes of Different Infertility Causes.

Zhang Y, He C, He Y, Zhu Z Reprod Sci. 2024; .

PMID: 39090336 DOI: 10.1007/s43032-024-01664-y.

The role of CoQ10 in embryonic development.

He X, Chen H, Liao M, Zhao X, Zhang D, Jiang M J Assist Reprod Genet. 2024; 41(3):767-779.

PMID: 38372883 PMC: 10957822. DOI: 10.1007/s10815-024-03052-6.

Metabolomic profiling of exosomes reveals age-related changes in ovarian follicular fluid.

Gu Y, Zhang X, Wang R, Wei Y, Peng H, Wang K Eur J Med Res. 2024; 29(1):4.

PMID: 38173013 PMC: 10762974. DOI: 10.1186/s40001-023-01586-6.

References

Burger H . Androgen production in women. Fertil Steril. 2002; 77 Suppl 4:S3-5. DOI: 10.1016/s0015-0282(02)02985-0. View

Nagy B, Poto L, Farkas N, Koppan M, Varnagy A, Kovacs K . Follicular fluid progesterone concentration is associated with fertilization outcome after IVF: a systematic review and meta-analysis. Reprod Biomed Online. 2019; 38(6):871-882. DOI: 10.1016/j.rbmo.2018.12.045. View

Roy S, Gandra D, Seger C, Biswas A, Kushnir V, Gleicher N . Oocyte-Derived Factors (GDF9 and BMP15) and FSH Regulate AMH Expression Via Modulation of H3K27AC in Granulosa Cells. Endocrinology. 2018; 159(9):3433-3445. PMC: 6112599. DOI: 10.1210/en.2018-00609. View

Tao Z, Song W, Zhu C, Xu W, Liu H, Zhang S . Comparative transcriptomic analysis of high and low egg-producing duck ovaries. Poult Sci. 2017; 96(12):4378-4388. DOI: 10.3382/ps/pex229. View

Jiao J, Shi B, Wang T, Fang Y, Cao T, Zhou Y . Characterization of long non-coding RNA and messenger RNA profiles in follicular fluid from mature and immature ovarian follicles of healthy women and women with polycystic ovary syndrome. Hum Reprod. 2018; 33(9):1735-1748. DOI: 10.1093/humrep/dey255. View

Kushnir M, Naessen T, Wanggren K, Hreinsson J, Rockwood A, Meikle A . Exploratory study of the association of steroid profiles in stimulated ovarian follicular fluid with outcomes of IVF treatment. J Steroid Biochem Mol Biol. 2015; 162:126-33. DOI: 10.1016/j.jsbmb.2015.09.015. View

Unal G, Erdogan E, Oguz A, Kaptaner B, Kankaya E, Elp M . Determination of hormones inducing oocyte maturation in Chalcalburnus tarichi (Pallas, 1811). Fish Physiol Biochem. 2008; 34(4):447-54. DOI: 10.1007/s10695-008-9203-8. View

Wu X, Pan X, Cao S, Xu F, Lan L, Zhang Y . iTRAQ-based quantitative proteomic analysis provides insights into strong broodiness in Muscovy duck (Cairina moschata) combined with metabolomics analysis. J Proteomics. 2019; 204:103401. DOI: 10.1016/j.jprot.2019.103401. View

Du Y, Guo N, Wang Y, Teng X, Hua X, Deng T . Follicular fluid concentrations of phthalate metabolites are associated with altered intrafollicular reproductive hormones in women undergoing in vitro fertilization. Fertil Steril. 2019; 111(5):953-961. DOI: 10.1016/j.fertnstert.2019.01.021. View

10.

Liu L, Yin T, Chen Y, Li Y, Yin L, Ding J . Follicular dynamics of glycerophospholipid and sphingolipid metabolisms in polycystic ovary syndrome patients. J Steroid Biochem Mol Biol. 2018; 185:142-149. DOI: 10.1016/j.jsbmb.2018.08.008. View

11.

Agarwal A, Gupta S, Sharma R . Role of oxidative stress in female reproduction. Reprod Biol Endocrinol. 2005; 3:28. PMC: 1215514. DOI: 10.1186/1477-7827-3-28. View

12.

Haider S, Inbaraj R . Relativein vitro effectiveness of estradiol-17β, androgens, corticosteroids, progesterone and other pregnene derivatives on germinal vesicle breakdown in oocytes of Indian major carps,Labeo rohita, Cirrhinus mrigala andCatla catla. Fish Physiol Biochem. 2013; 6(5):289-95. DOI: 10.1007/BF01881683. View

13.

Celik E, Celik O, Kumbak B, Yilmaz E, Turkcuoglu I, Simsek Y . A comparative study on oxidative and antioxidative markers of serum and follicular fluid in GnRH agonist and antagonist cycles. J Assist Reprod Genet. 2012; 29(11):1175-83. PMC: 3510367. DOI: 10.1007/s10815-012-9843-6. View

14.

Perovic M, Sudar-Milovanovic E, Simonovic E, Resanovic I, Draganic V, Radakovic J . Hypothesis regarding the effects of gonadotropins on the level of free fatty acids and phospholipids in serum and follicular fluid during controlled ovarian stimulation. Med Hypotheses. 2019; 123:30-34. DOI: 10.1016/j.mehy.2018.11.021. View

15.

Khan H, Bhatti S, Suhail S, Gul R, Awais A, Hamayun H . Antral follicle count (AFC) and serum anti-Müllerian hormone (AMH) are the predictors of natural fecundability have similar trends irrespective of fertility status and menstrual characteristics among fertile and infertile women below the age of 40.... Reprod Biol Endocrinol. 2019; 17(1):20. PMC: 6371573. DOI: 10.1186/s12958-019-0464-0. View

16.

Marei W, Van Raemdonck G, Baggerman G, Bols P, Leroy J . Proteomic changes in oocytes after in vitro maturation in lipotoxic conditions are different from those in cumulus cells. Sci Rep. 2019; 9(1):3673. PMC: 6403224. DOI: 10.1038/s41598-019-40122-7. View

17.

Wirleitner B, Okhowat J, Vistejnova L, Kralickova M, Karlikova M, Vanderzwalmen P . Relationship between follicular volume and oocyte competence, blastocyst development and live-birth rate: optimal follicle size for oocyte retrieval. Ultrasound Obstet Gynecol. 2017; 51(1):118-125. DOI: 10.1002/uog.18955. View

18.

Chen M, Zhang B, Cai S, Zeng X, Ye Q, Mao X . Metabolic disorder of amino acids, fatty acids and purines reflects the decreases in oocyte quality and potential in sows. J Proteomics. 2019; 200:134-143. DOI: 10.1016/j.jprot.2019.03.015. View

19.

Castiglione Morelli M, Iuliano A, Schettini S, Petruzzi D, Ferri A, Colucci P . NMR metabolic profiling of follicular fluid for investigating the different causes of female infertility: a pilot study. Metabolomics. 2019; 15(2):19. DOI: 10.1007/s11306-019-1481-x. View

20.

Zuelke K, Jeffay S, Zucker R, Perreault S . Glutathione (GSH) concentrations vary with the cell cycle in maturing hamster oocytes, zygotes, and pre-implantation stage embryos. Mol Reprod Dev. 2002; 64(1):106-12. DOI: 10.1002/mrd.10214. View