Identification of Biomarkers for Sarcoidosis and Tuberculosis of the Lung Using Systematic and Integrated Analysis

Overview

Journal Med Sci Monit

Specialties General Medicine
Pathology

Date 2020 Jul 24

PMID 32701935

Citations 5

Authors

Min Zhao

Xin Di

Xin Jin

Chang Tian

Shan Cong

Jiaying Liu

Ke Wang

Affiliations

Soon will be listed here.

Abstract

BACKGROUND Sarcoidosis (SARC) is a multisystem inflammatory disease of unknown etiology and pulmonary tuberculosis (PTB) is caused by Mycobacterium tuberculosis. Both of these diseases affect lungs and lymph nodes and share similar clinical manifestations. However, the underlying mechanisms for the similarities and differences in genetic characteristics of SARC and PTB remain unclear. MATERIAL AND METHODS Three datasets (GSE16538, GSE20050, and GSE19314) were retrieved from the Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) in SARC and PTB were identified using GEO2R online analyzer and Venn diagram software. Functional enrichment analysis was performed using Database for Annotation, Visualization and Integrated Discovery (DAVID) and R packages. Two protein-protein interaction (PPI) networks were constructed using Search Tool for the Retrieval of Interacting Genes database, and module analysis was performed using Cytoscape. Hub genes were identified using area under the receiver operating characteristic curve analysis. RESULTS We identified 228 DEGs, including 56 common SARC-PTB DEGs (enriched in interferon-gamma-mediated signaling, response to gamma radiation, and immune response) and 172 SARC-only DEGs (enriched in immune response, cellular calcium ion homeostasis, and dendritic cell chemotaxis). Potential biomarkers for SARC included CBX5, BCL11B, and GPR18. CONCLUSIONS We identified potential biomarkers that can be used as candidates for diagnosis and/or treatment of patients with SARC.

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References

Gibejova A, Mrazek F, Subrtova D, Sekerova V, Szotkowska J, Kolek V . Expression of macrophage inflammatory protein-3 beta/CCL19 in pulmonary sarcoidosis. Am J Respir Crit Care Med. 2003; 167(12):1695-703. DOI: 10.1164/rccm.200205-487OC. View

Perez R, Rivera-Marrero C, Roman J . Pulmonary granulomatous inflammation: From sarcoidosis to tuberculosis. Semin Respir Infect. 2003; 18(1):23-32. DOI: 10.1053/srin.2003.50005. View

Martens H, Zollner B, Zissel G, Burdon D, Schlaak M, Muller-Quernheim J . Anti-Borrelia burgdorferi immunoglobulin seroprevalence in pulmonary sarcoidosis: a negative report. Eur Respir J. 1997; 10(6):1356-8. DOI: 10.1183/09031936.97.10061356. View

Planck A, Eklund A, Grunewald J, Vene S . No serological evidence of Rickettsia helvetica infection in Scandinavian sarcoidosis patients. Eur Respir J. 2004; 24(5):811-3. DOI: 10.1183/09031936.04.00014604. View

Derler A, Eisendle K, Baltaci M, Obermoser G, Zelger B . High prevalence of 'Borrelia-like' organisms in skin biopsies of sarcoidosis patients from Western Austria. J Cutan Pathol. 2009; 36(12):1262-8. DOI: 10.1111/j.1600-0560.2009.01271.x. View

Iijima Y, Sugiyama Y, Sawahata M, Nakayama M, Bando M . Clinical Features of Pulmonary Sarcoidosis Complicated by Lung Cancer. Intern Med. 2017; 56(15):1957-1960. PMC: 5577069. DOI: 10.2169/internalmedicine.56.7047. View

Biberfeld P, Petren A, Eklund A, Lindemalm C, Barkhem T, Ekman M . Human herpesvirus-6 (HHV-6, HBLV) in sarcoidosis and lymphoproliferative disorders. J Virol Methods. 1988; 21(1-4):49-59. DOI: 10.1016/0166-0934(88)90051-1. View

Tsuchiya K, Karayama M, Sato T, Yasui H, Hozumi H, Suzuki Y . Simultaneous Occurrence of Sarcoidosis and Anti-neutrophil Cytoplasmic Antibody-associated Vasculitis in a Patient with Lung Cancer. Intern Med. 2019; 58(22):3299-3304. PMC: 6911755. DOI: 10.2169/internalmedicine.3004-19. View

Hong S, Mok Y, Jeon C, Jee S, Samet J . Tuberculosis, smoking and risk for lung cancer incidence and mortality. Int J Cancer. 2016; 139(11):2447-55. DOI: 10.1002/ijc.30384. View

10.

Svendsen C, Milman N, Nielsen H, Krogfelt K, Larsen K . A prospective study evaluating the presence of Rickettsia in Danish patients with sarcoidosis. Scand J Infect Dis. 2009; 41(10):745-52. DOI: 10.1080/00365540903177727. View

11.

Nourbakhsh F, Atabaki R, Roohbakhsh A . The role of orphan G protein-coupled receptors in the modulation of pain: A review. Life Sci. 2018; 212:59-69. DOI: 10.1016/j.lfs.2018.09.028. View

12.

Tokuyasu H, Izumi H, Mukai N, Takeda K, Sakaguchi Y, Isowa N . Small cell lung cancer complicated by pulmonary sarcoidosis. Intern Med. 2010; 49(18):1997-2001. DOI: 10.2169/internalmedicine.49.3797. View

13.

Gupta D, Agarwal R, Aggarwal A, Jindal S . Molecular evidence for the role of mycobacteria in sarcoidosis: a meta-analysis. Eur Respir J. 2007; 30(3):508-16. DOI: 10.1183/09031936.00002607. View

14.

McNeill M, Zanders T, Morris M . A 49-year-old man with concurrent diagnoses of lung cancer, sarcoidosis, and multiple regions of adenopathy on positron emission tomography. Chest. 2009; 135(2):546-549. DOI: 10.1378/chest.08-1221. View

15.

Guo T, Ma H, Zhou Y . Bioinformatics analysis of microarray data to identify the candidate biomarkers of lung adenocarcinoma. PeerJ. 2019; 7:e7313. PMC: 6626531. DOI: 10.7717/peerj.7313. View

16.

Alberg A, Brock M, Ford J, Samet J, Spivack S . Epidemiology of lung cancer: Diagnosis and management of lung cancer, 3rd ed: American College of Chest Physicians evidence-based clinical practice guidelines. Chest. 2013; 143(5 Suppl):e1S-e29S. PMC: 4694610. DOI: 10.1378/chest.12-2345. View

17.

Chang S, Mirabolfathinejad S, Katta H, Cumpian A, Gong L, Caetano M . T helper 17 cells play a critical pathogenic role in lung cancer. Proc Natl Acad Sci U S A. 2014; 111(15):5664-9. PMC: 3992670. DOI: 10.1073/pnas.1319051111. View

18.

Maertzdorf J, Weiner 3rd J, Mollenkopf H, Bauer T, Prasse A, Muller-Quernheim J . Common patterns and disease-related signatures in tuberculosis and sarcoidosis. Proc Natl Acad Sci U S A. 2012; 109(20):7853-8. PMC: 3356621. DOI: 10.1073/pnas.1121072109. View

19.

Landi C, Carleo A, Cillis G, Rottoli P . Sarcoidosis: proteomics and new perspectives for improving personalized medicine. Expert Rev Proteomics. 2018; 15(10):829-835. DOI: 10.1080/14789450.2018.1528148. View

20.

Arger N, Ho M, Woodruff P, Koth L . Serum CXCL11 correlates with pulmonary outcomes and disease burden in sarcoidosis. Respir Med. 2019; 152:89-96. PMC: 6594698. DOI: 10.1016/j.rmed.2019.04.005. View