Evaluation of the Effects of Paclobutrazol and Cultivation Years on Saponins in Using UPLC-ELSD

Overview

Journal Int J Anal Chem

Publisher Wiley

Specialty Chemistry

Date 2020 Jul 23

PMID 32695173

Citations 3

Authors

Peng Sun

Juhua Tong

Xianen Li

Affiliations

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Abstract

Nowadays, there is a growing concern about the quality of herbs used in traditional Chinese medicine. In this study, we evaluated the impacts of paclobutrazol and cultivation period on steroid saponins in . A rapid method to simultaneously determine three principle steroid saponins (ophiopogonins B, D, and D') using ultraperformance liquid chromatography combined with an evaporative light-scattering detector was developed. The contents of three saponins in paclobutrazol-treated and nontreated Sichuan and those in the 2-year and 3-year Zhejiang were analyzed. The results showed that the saponin contents were sharply reduced in paclobutrazol-treated as compared to the control, whereas the concentrations of the three targeted saponins in Zhejiang varied with the increase in cultivation years, reflecting varied effects on saponins. Our study provided chemical evidences for further quality control and agricultural practices of .

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