The Effect of Spore Oil in Early Skin Wound Healing: Interactions of Skin Microbiota and Inflammation

Overview

Journal Aging (Albany NY)

Specialty Geriatrics

Date 2020 Jul 22

PMID 32692722

Citations 9

Authors

Chunwei Jiao

Yizhen Xie

Hao Yun

Huijia Liang

Chunyan He

Aimin Jiang

Qingping Wu

Burton B Yang

Affiliations

Soon will be listed here.

Abstract

The mushroom has been a traditional Chinese medicine for millennia. In this study, we isolated the spore oil (GLSO) and evaluated the effect of GLSO on skin burn wound healing and the underlying mechanisms. Mice were used to perform skin wound healing assay. Wound analysis was performed by photography, hematoxylin/eosin staining, Masson's Trichrome staining and immunohistochemical analysis. Microbiota on the wounds were analyzed using the 16s rRNA sequence and quantitative statistics. The lipopolysaccharide (LPS) content was examined in skin wounds and serum using an enzyme-linked immunosorbent assay (ELISA). The expression of Toll-like receptor 4 (TLR4) and the relative levels of inflammatory cytokines were determined by qPCR and immunofluorescence assay. A pseudo-germfree mouse model treated with antibiotics was used to investigate whether GLSO accelerated skin burn wound healing through the skin microbiota. We found that GLSO significantly accelerated the process of skin wound healing and regulated the levels of gram-negative and gram-positive bacteria. Furthermore, GLSO reduced LPS and TLR4, and levels of some other related inflammatory cytokines. The assay with the pseudo-germfree mice model showed that GLSO had a significant acceleration on skin wound healing in comparison with antibiotic treatment. Thus, GLSO downregulated the inflammation by regulating skin microbiota to accelerate skin wound healing. These findings provide a scientific rationale for the potential therapeutic use of GLSO in skin burn injury.

Citing Articles

Bioactive components, pharmacological properties and underlying mechanism of spore oil: A review.

Liu J, Zhang B, Wang L, Li S, Long Q, Xiao X Chin Herb Med. 2024; 16(3):375-391.

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Pharmacological Activities and Safety of Ganoderma lucidum Spores: A Systematic Review.

Thuy N, Tu V, Thu L, Giang T, Huyen D, Loc D Cureus. 2023; 15(9):e44574.

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Protective effect of spore oil-functionalized nano-selenium system on cisplatin-induced nephrotoxicity by regulating oxidative stress-mediated pathways and activating immune response.

Liu C, Zhou S, Lai H, Shi L, Bai W, Li X J Nanobiotechnology. 2023; 21(1):47.

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Efficacy of Human-Recombinant Epidermal Growth Factor Combined with Povidone-Iodine for Pressure Ulcers and Its Influence on Inflammatory Cytokines.

Zhao Z, Lv D, Zhang B, Yong L, Zhang R, Wang X Mediators Inflamm. 2022; 2022:3878320.

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Poloxamer 407 and Hyaluronic Acid Thermosensitive Hydrogel-Encapsulated Ginsenoside Rg3 to Promote Skin Wound Healing.

Peng X, Ding C, Zhao Y, Hao M, Liu W, Yang M Front Bioeng Biotechnol. 2022; 10:831007.

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