Establishment of Day 7 Blastocyst Freezing Criteria Using Blastocyst Diameter for Single Vitrified-warmed Blastocyst Transfer from Live Birth Outcomes: a Single-center, Large Cohort, Retrospectively Matched Study

Overview

Journal J Assist Reprod Genet

Publisher Springer

Specialties Genetics
Reproductive Medicine

Date 2020 Jul 22

PMID 32691193

Citations 8

Authors

Satoshi Ueno

Kazuo Uchiyama

Tomoko Kuroda

Tadashi Okimura

Akiko Yabuuchi

Tamotsu Kobayashi

Keiichi Kato

Affiliations

Soon will be listed here.

Abstract

Purpose: To establish blastocyst freezing criteria for day 7 blastocyst (day 7 BL) for single vitrified-warmed blastocyst transfer (SVBT) by examining the diameter of blastocysts.

Methods: Patients who underwent day 7 BL transfer cycles (1143 cycles, mean age: 38.5 ± 3.5) and randomly selected patients after 1:1 matching who underwent day 6 BL transfer cycles and day 2-single-embryo transfer (SET) cycles were used for analysis. Comparison of the miscarriage (per clinical pregnancy) and live birth rates were made among day 2-SET, day 7 BL, and day 6 BL. These blastocyst groups were stratified into six groups based on blastocyst diameter, namely, 180 μm, 190 μm, 200 μm, 210 μm, over 220 μm, and hatched, for making the freezing criteria.

Results: For each diameter, 180 μm, 190 μm, 200 μm, 210 μm, over 220 μm, and hatched, the live birth rates of day 7 BL after SVBT were 9.0%, 11.9%, 11.5%, 15.6%, 20.0%, and 19.9%, respectively. Compared with the 14.6% live birth rate of the day 2-SET group, the live birth rate of 220 μm day 7 BL was significantly higher (P < 0.05) and was around the same in other diameter groups.

Conclusion: Our study demonstrates that sufficient live birth rates can be obtained after SVBT even from blastocysts on day 7 when blastocysts were vitrified at expanded blastocyst stage of over 180 μm of diameter or at hatched blastocyst stage and were transferred at the optimal time. This is the first study to establish a day 7 blastocyst freezing criteria using blastocyst diameter, which is an objective assessment way.

Citing Articles

Time-lapse imaging of morula compaction for selecting high-quality blastocysts: a retrospective cohort study.

Park J, Jeon Y, Bang S, Kim J, Kwak I, Lee W Arch Gynecol Obstet. 2024; 309(6):2897-2906.

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Development and validation of a nomogram for predicting ongoing pregnancy in single vitrified-warmed blastocyst embryo transfer cycles.

Park J, Park J, Bang S, Jeon H, Kim J, Lee W Front Endocrinol (Lausanne). 2023; 14:1257764.

PMID: 38075065 PMC: 10702135. DOI: 10.3389/fendo.2023.1257764.

Pre-Vitrification and Post-Warming Variables of Vitrified-Warmed Blastocysts That Are Predictable for Implantation.

Gabrielsen A, Iversen L, Fedder J, Eskildsen T, Englund A, Hansen S J Clin Med. 2023; 12(19).

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Comparison of 1-year cumulative live birth and perinatal outcomes following single blastocyst transfer with or without preimplantation genetic testing for aneuploidy: a propensity score-matched study.

Kato K, Ezoe K, Onogi S, Ito S, Egawa R, Aoyama N J Assist Reprod Genet. 2023; 40(11):2669-2680.

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Clinical outcome analysis of frozen-thawed embryo transfer on Day 7.

Liu X, Lou H, Zhang J, Du M, Du Y, Wu S Front Endocrinol (Lausanne). 2022; 13:1082597.

PMID: 36568064 PMC: 9780262. DOI: 10.3389/fendo.2022.1082597.

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