Isolation of TSCD11 Gene for Early Chloroplast Development Under High Temperature in Rice

Overview

Journal Rice (N Y)

Date 2020 Jul 19

PMID 32681435

Citations 3

Authors

Guonan Fang

Shenglong Yang

Banpu Ruan

Chaolei Liu

Anpeng Zhang

Hongzhen Jiang

Shilin Ding

Biao Tian

Yu Zhang

Noushin Jahan

Li Zhu

Guangheng Zhang

Guojun Dong

Qiang Zhang

Dali Zeng

Longbiao Guo

Zhenyu Gao

Qian Qian

Affiliations

Soon will be listed here.

Abstract

Background: Chloroplasts are essential for photosynthesis and play key roles in plant development. High temperature affects structure of chloroplasts and metabolism in plants. The seryl-tRNA synthetase plays an important role in translation of proteins. Although seryl-tRNA synthetase has been widely studied in microbes and animals, few studies have reported about its role in chloroplast development under high temperature in rice.

Results: In this study, we isolated a novel temperature-sensitive chlorophyll-deficient 11 (tscd11) mutant by ethyl methane sulfonate (EMS) mutagenesis of japonica variety Wuyujing7. The tscd11 mutant developed albino leaves at the 3-leaf stage under high temperature (35 °C), but had normal green leaves under low temperature (25 °C). Consistent with the albino phenotype, impaired chloroplasts, decreased chlorophyll content and increased ROS accumulation were found in the tscd11 mutant at 35 °C. Fine mapping and DNA sequencing of tscd11 revealed a missense mutation (G to A) in the eighth exon of LOC_Os11g39670 resulted in amino acid change (Glu to Lys). The TSCD11 gene encodes a seryl-tRNA synthetase localized to chloroplast. Complementation test confirmed that the point mutation in TSCD11 is responsible for the phenotype of tscd11. TSCD11 is highly expressed in leaves. Compared with the wild type (WT), mutation in TSCD11 led to significant alteration in expression levels of genes associated with chlorophyll biosynthesis, photosynthesis and chloroplast development under high temperature.

Conclusions: TSCD11, encoding a seryl-tRNA synthetase localized to chloroplast, is vital to early chloroplast development at high temperature in rice, which help to further study on the molecular mechanism of chloroplast development under high temperature.

Citing Articles

Comparative Analysis of the Chloroplast Genomes of the (Styracaceae) Species: Providing Insights into Molecular Evolution and Phylogenetic Relationships.

Dai W, Zheng H, Xu M, Zhu X, Long H, Xu X Int J Mol Sci. 2025; 26(1.

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The Rice Aspartyl-tRNA Synthetase YLC3 Regulates Amino Acid Homeostasis and Chloroplast Development Under Low Temperature.

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LSL1 controls cell death and grain production by stabilizing chloroplast in rice.

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PMID: 35960419 DOI: 10.1007/s11427-022-2152-6.

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References

Huang L, Sun Q, Qin F, Li C, Zhao Y, Zhou D . Down-regulation of a SILENT INFORMATION REGULATOR2-related histone deacetylase gene, OsSRT1, induces DNA fragmentation and cell death in rice. Plant Physiol. 2007; 144(3):1508-19. PMC: 1914135. DOI: 10.1104/pp.107.099473. View

Allakhverdiev S, Kreslavski V, Klimov V, Los D, Carpentier R, Mohanty P . Heat stress: an overview of molecular responses in photosynthesis. Photosynth Res. 2008; 98(1-3):541-50. DOI: 10.1007/s11120-008-9331-0. View

Liu Y, He J, Chen Z, Ren X, Hong X, Gong Z . ABA overly-sensitive 5 (ABO5), encoding a pentatricopeptide repeat protein required for cis-splicing of mitochondrial nad2 intron 3, is involved in the abscisic acid response in Arabidopsis. Plant J. 2010; 63(5):749-65. DOI: 10.1111/j.1365-313X.2010.04280.x. View

Lee S, Kim J, Yoo E, Lee C, Hirochika H, An G . Differential regulation of chlorophyll a oxygenase genes in rice. Plant Mol Biol. 2005; 57(6):805-18. DOI: 10.1007/s11103-005-2066-9. View

Moradi F, Ismail A . Responses of photosynthesis, chlorophyll fluorescence and ROS-scavenging systems to salt stress during seedling and reproductive stages in rice. Ann Bot. 2007; 99(6):1161-73. PMC: 3243573. DOI: 10.1093/aob/mcm052. View

Chen G, Wu C, He L, Qiu Z, Zhang S, Zhang Y . Knocking Out the Gene Induces Hypersensitivity to Oxidative Stress and Premature Leaf Senescence in Rice. Int J Mol Sci. 2018; 19(10). PMC: 6213272. DOI: 10.3390/ijms19102853. View

Baxter A, Mittler R, Suzuki N . ROS as key players in plant stress signalling. J Exp Bot. 2013; 65(5):1229-40. DOI: 10.1093/jxb/ert375. View

Wu Z, Zhang X, He B, Diao L, Sheng S, Wang J . A chlorophyll-deficient rice mutant with impaired chlorophyllide esterification in chlorophyll biosynthesis. Plant Physiol. 2007; 145(1):29-40. PMC: 1976586. DOI: 10.1104/pp.107.100321. View

Bohra-Mishra P, Oppenheimer M, Hsiang S . Nonlinear permanent migration response to climatic variations but minimal response to disasters. Proc Natl Acad Sci U S A. 2014; 111(27):9780-5. PMC: 4103331. DOI: 10.1073/pnas.1317166111. View

10.

Lopez-Juez E . Plastid biogenesis, between light and shadows. J Exp Bot. 2006; 58(1):11-26. DOI: 10.1093/jxb/erl196. View

11.

Szymanski M, Deniziak M, Barciszewski J . The new aspects of aminoacyl-tRNA synthetases. Acta Biochim Pol. 2001; 47(3):821-34. View

12.

Peng S, Huang J, Sheehy J, Laza R, Visperas R, Zhong X . Rice yields decline with higher night temperature from global warming. Proc Natl Acad Sci U S A. 2004; 101(27):9971-5. PMC: 454199. DOI: 10.1073/pnas.0403720101. View

13.

Yang X, Li Y, Qi M, Liu Y, Li T . Targeted Control of Chloroplast Quality to Improve Plant Acclimation: From Protein Import to Degradation. Front Plant Sci. 2019; 10:958. PMC: 6670758. DOI: 10.3389/fpls.2019.00958. View

14.

Jacob P, Hirt H, Bendahmane A . The heat-shock protein/chaperone network and multiple stress resistance. Plant Biotechnol J. 2016; 15(4):405-414. PMC: 5362687. DOI: 10.1111/pbi.12659. View

15.

Qiu Z, Zhu L, He L, Chen D, Zeng D, Chen G . DNA damage and reactive oxygen species cause cell death in the rice local lesions 1 mutant under high light and high temperature. New Phytol. 2018; 222(1):349-365. DOI: 10.1111/nph.15597. View

16.

Yamamoto Y, Aminaka R, Yoshioka M, Khatoon M, Komayama K, Takenaka D . Quality control of photosystem II: impact of light and heat stresses. Photosynth Res. 2008; 98(1-3):589-608. DOI: 10.1007/s11120-008-9372-4. View

17.

Wang Y, Wang C, Zheng M, Lyu J, Xu Y, Li X . WHITE PANICLE1, a Val-tRNA Synthetase Regulating Chloroplast Ribosome Biogenesis in Rice, Is Essential for Early Chloroplast Development. Plant Physiol. 2016; 170(4):2110-23. PMC: 4825129. DOI: 10.1104/pp.15.01949. View

18.

Schimmel P, Soll D . Aminoacyl-tRNA synthetases: general features and recognition of transfer RNAs. Annu Rev Biochem. 1979; 48:601-48. DOI: 10.1146/annurev.bi.48.070179.003125. View

19.

Yoo S, Cho S, Sugimoto H, Li J, Kusumi K, Koh H . Rice virescent3 and stripe1 encoding the large and small subunits of ribonucleotide reductase are required for chloroplast biogenesis during early leaf development. Plant Physiol. 2009; 150(1):388-401. PMC: 2675711. DOI: 10.1104/pp.109.136648. View

20.

Qiu Z, Kang S, He L, Zhao J, Zhang S, Hu J . The newly identified heat-stress sensitive albino 1 gene affects chloroplast development in rice. Plant Sci. 2018; 267:168-179. DOI: 10.1016/j.plantsci.2017.11.015. View