MIST: a Multilocus Identification System for

Due to the rapid expansion in microbial taxonomy, precise identification of common industrially and agriculturally relevant fungi such as species is challenging. In this study, we introduce the online multilocus identification system (MIST) for automated detection of 349 species based on a set of three DNA barcodes. MIST is based on the reference databases of validated sequences of three commonly used phylogenetic markers collected from public databases. The databases consist of 414 complete sequences of the nuclear rRNA internal transcribed spacers (ITS) 1 and 2, 583 sequence fragments of the gene encoding translation elongation factor 1-alpha (), and 534 sequence fragments of the gene encoding RNA polymerase subunit 2 (). Through MIST, information from different DNA barcodes can be combined and the identification of species can be achieved based on the integrated parametric sequence similarity search (blastn) performed in the manner of a decision tree classifier. In the verification process, MIST provided correct identification for 44 species based on DNA barcodes consisting of and markers. Thus, MIST can be used to obtain an automated species identification as well as to retrieve sequences required for manual identification by means of phylogenetic analysis. The genus is important to humankind, with a wide range of applications in industry, agriculture, and bioremediation. Thus, quick and accurate identification of species is paramount, since it is usually the first step in -based research. However, it frequently becomes a limitation, especially for researchers who lack taxonomic knowledge of fungi. Moreover, as the number of -based studies has increased, a growing number of unidentified sequences have been stored in public databases, which has made the species identification more ambiguous. In this study, we provide an easy-to-use tool, MIST, for automated species identification, a list of species, and corresponding sequences of reference DNA barcodes. Therefore, this study will facilitate the research on the biodiversity and applications of the genus .