The Regulatory Role of MiR-107 in Coxsackie B3 Virus Replication

Overview

Journal Aging (Albany NY)

Specialty Geriatrics

Date 2020 Jul 17

PMID 32674073

Citations 6

Authors

Min Yao

Chi Xu

Hongxing Shen

Tingjun Liu

Xiuping Wang

Chen Shao

Shihe Shao

Affiliations

Soon will be listed here.

Abstract

Coxsackie B3 virus (CVB3) is a member of small RNA viruses that belongs to the genus Enterovirus of the family Picornaviridae and CVB3 is the main pathogen of acute and chronic viral myocarditis. In this study RT-qPCR was used to determine the expression of miR-107 in CVB3-infected and uninfected HeLa cells. The experimental results show that the level of miR-107 began to rise at 4 h after the infection, and significantly boosted at 6 h. Based on the results of this experiment, we consider that miR-107 expression is related to CVB3 infection. In order to further clarify the effect of miR-107 in the process of CVB3 infection, we studied the effect of miR-107 upstream and downstream target genes on CVB3 replication. Levels of the target RNAs were detected by RT-qPCR after CVB3 infection, and the expression of CVB3 capsid protein VP1 by western blot analysis. Then the virus in the supernatant was quantitated via a viral plaque assay, reflecting the release of the virus. The experimental results showed that miRNA-107 expression is associated with CVB3 replication and proliferation, while KLF4 and BACE1 as the downstream of miR-107 weakened CVB3 replication. Overexpressions of KLF4 and BACE1 negatively regulated CVB3 replication, this effect on CVB3 was completely opposite to that of miR-107. Further experiments revealed that the upstream lncRNA004787, a new lncRNA that had not been reported, was located on chromosome 5, strand - from 37073250 to 37070908 (genome assembly: hg19). We sequenced and studied lncRNA004787 and found that it partially inhibited CVB3 replication. This prompted us to speculate that lncRNA004787 probably impacted the replication by other means. In conclusion, miR-107 interfered with CVB3 replication and release.

Citing Articles

Role of non-coding RNAs in the pathogenesis of viral myocarditis.

Kang Z, Zhang L, Yang Z Virulence. 2025; 16(1):2466480.

PMID: 39950847 PMC: 11849450. DOI: 10.1080/21505594.2025.2466480.

Application of the Trend of miRNA Expression Levels in APP/PS1 Mice Plasma for the Early Diagnosis of Alzheimer's Disease.

Zhang Y, Wang Y, Zhao F Mol Neurobiol. 2025; .

PMID: 39937416 DOI: 10.1007/s12035-025-04743-6.

Research progress on miRNAs function in the interaction between human infectious viruses and hosts: A review.

Wang X, Zhao W Biomol Biomed. 2024; 24(6):1452-1462.

PMID: 39101759 PMC: 11496870. DOI: 10.17305/bb.2024.10821.

A Narrative Review of Preclinical In Vitro Studies Investigating microRNAs in Myocarditis.

Grodzka O, Procyk G, Wrzosek M Curr Issues Mol Biol. 2024; 46(2):1413-1423.

PMID: 38392209 PMC: 10887635. DOI: 10.3390/cimb46020091.

MicroRNA-22-3p displaces critical host factors from the 5' UTR and inhibits the translation of Coxsackievirus B3 RNA.

Rani P, George B, V S, Biswas S, V M, Pal A J Virol. 2024; 98(2):e0150423.

PMID: 38289119 PMC: 10883805. DOI: 10.1128/jvi.01504-23.

References

Shi S, Wang L, Yu B, Li Y, Jin Y, Bai X . LncRNA-ATB promotes trastuzumab resistance and invasion-metastasis cascade in breast cancer. Oncotarget. 2015; 6(13):11652-63. PMC: 4484483. DOI: 10.18632/oncotarget.3457. View

Fitzgerald K, Caffrey D . Long noncoding RNAs in innate and adaptive immunity. Curr Opin Immunol. 2014; 26:140-6. PMC: 3932021. DOI: 10.1016/j.coi.2013.12.001. View

Horita K, Kurosaki H, Nakatake M, Ito M, Kono H, Nakamura T . Long noncoding RNA UCA1 enhances sensitivity to oncolytic vaccinia virus by sponging miR-18a/miR-182 and modulating the Cdc42/filopodia axis in colorectal cancer. Biochem Biophys Res Commun. 2019; 516(3):831-838. DOI: 10.1016/j.bbrc.2019.06.125. View

Wang P, Xu J, Wang Y, Cao X . An interferon-independent lncRNA promotes viral replication by modulating cellular metabolism. Science. 2017; 358(6366):1051-1055. DOI: 10.1126/science.aao0409. View

Paraskevopoulou M, Hatzigeorgiou A . Analyzing MiRNA-LncRNA Interactions. Methods Mol Biol. 2016; 1402:271-286. DOI: 10.1007/978-1-4939-3378-5_21. View

Kambara H, Niazi F, Kostadinova L, Moonka D, Siegel C, Post A . Negative regulation of the interferon response by an interferon-induced long non-coding RNA. Nucleic Acids Res. 2014; 42(16):10668-80. PMC: 4176326. DOI: 10.1093/nar/gku713. View

Ye X, Hemida M, Qiu Y, Hanson P, Zhang H, Yang D . MiR-126 promotes coxsackievirus replication by mediating cross-talk of ERK1/2 and Wnt/β-catenin signal pathways. Cell Mol Life Sci. 2013; 70(23):4631-44. PMC: 11113642. DOI: 10.1007/s00018-013-1411-4. View

Zheng L, Zhang Y, Fu Y, Gong H, Guo J, Wu K . Long non-coding RNA MALAT1 regulates BLCAP mRNA expression through binding to miR-339-5p and promotes poor prognosis in breast cancer. Biosci Rep. 2019; 39(2). PMC: 6379223. DOI: 10.1042/BSR20181284. View

Zhong M, Chen Y, Zhang G, Xu L, Ge W, Wu B . LncRNA H19 regulates PI3K-Akt signal pathway by functioning as a ceRNA and predicts poor prognosis in colorectal cancer: integrative analysis of dysregulated ncRNA-associated ceRNA network. Cancer Cell Int. 2019; 19:148. PMC: 6543669. DOI: 10.1186/s12935-019-0866-2. View

10.

Carnero E, Barriocanal M, Prior C, Unfried J, Segura V, Guruceaga E . Long noncoding RNA EGOT negatively affects the antiviral response and favors HCV replication. EMBO Rep. 2016; 17(7):1013-28. PMC: 4931568. DOI: 10.15252/embr.201541763. View

11.

Li Y, Wang H, Huang H . Long non-coding RNA MIR205HG function as a ceRNA to accelerate tumor growth and progression via sponging miR-122-5p in cervical cancer. Biochem Biophys Res Commun. 2019; 514(1):78-85. DOI: 10.1016/j.bbrc.2019.04.102. View

12.

Holmes A, Semler B . Picornaviruses and RNA Metabolism: Local and Global Effects of Infection. J Virol. 2019; 93(21). PMC: 6803262. DOI: 10.1128/JVI.02088-17. View

13.

Wu H, Zhuo Y, Zhou Y, Wang X, Wang Y, Si C . miR-29a promotes hepatitis B virus replication and expression by targeting SMARCE1 in hepatoma carcinoma. World J Gastroenterol. 2017; 23(25):4569-4578. PMC: 5504372. DOI: 10.3748/wjg.v23.i25.4569. View

14.

Amaral P, Clark M, Gascoigne D, Dinger M, Mattick J . lncRNAdb: a reference database for long noncoding RNAs. Nucleic Acids Res. 2010; 39(Database issue):D146-51. PMC: 3013714. DOI: 10.1093/nar/gkq1138. View

15.

Chen S, Hu X, Cui I, Wu S, Dou C, Liu Y . An endogenous retroviral element exerts an antiviral innate immune function via the derived lncRNA lnc-ALVE1-AS1. Antiviral Res. 2019; 170:104571. DOI: 10.1016/j.antiviral.2019.104571. View

16.

Li X, Kim S, Hong D, Jung K, Choi C, Seo Y . KLF4 suppresses the tumor activity of cutaneous squamous cell carcinoma (SCC) cells via the regulation of SMAD signaling and SOX2 expression. Biochem Biophys Res Commun. 2019; 516(4):1110-1115. DOI: 10.1016/j.bbrc.2019.07.011. View

17.

Feng T, Tammineni P, Agrawal C, Jeong Y, Cai Q . Autophagy-mediated Regulation of BACE1 Protein Trafficking and Degradation. J Biol Chem. 2016; 292(5):1679-1690. PMC: 5290944. DOI: 10.1074/jbc.M116.766584. View

18.

Mao Y, Liu R, Zhou H, Yin S, Zhao Q, Ding X . Transcriptome analysis of miRNA-lncRNA-mRNA interactions in the malignant transformation process of gastric cancer initiation. Cancer Gene Ther. 2017; 24(6):267-275. DOI: 10.1038/cgt.2017.14. View

19.

Tong L, Lin L, Wu S, Guo Z, Wang T, Qin Y . MiR-10a* up-regulates coxsackievirus B3 biosynthesis by targeting the 3D-coding sequence. Nucleic Acids Res. 2013; 41(6):3760-71. PMC: 3616696. DOI: 10.1093/nar/gkt058. View

20.

Fan J, Cheng M, Chi X, Liu X, Yang W . A Human Long Non-coding RNA LncATV Promotes Virus Replication Through Restricting RIG-I-Mediated Innate Immunity. Front Immunol. 2019; 10:1711. PMC: 6658999. DOI: 10.3389/fimmu.2019.01711. View