Characterization of Ca2+ Fluxes in Rat Liver Plasma-membrane Vesicles

Overview

Journal Biochem J

Specialty Biochemistry

Date 1988 Nov 15

PMID 3265619

Citations 2

Authors

C Dargemont

M Hilly

M Claret

J P Mauger

Affiliations

Soon will be listed here.

Abstract

Inside-out plasma-membrane vesicles isolated from rat liver [Prpic, Green, Blackmore & Exton (1984) J. Biol. Chem. 259, 1382-1385] accumulated a substantial amount of 45Ca2+ when they were incubated in a medium whose ionic composition and pH mimicked those of cytosol and which contained MgATP. The Vmax of the initial 45Ca2+ uptake rate was 2.9 +/- 0.6 nmol/min per mg and the Km for Ca2+ was 0.50 +/- 0.08 microM. The ATP-dependent 45Ca2+ uptake by inside-out plasma-membrane vesicles was about 20 times more sensitive to saponin than was the ATP-dependent uptake by a microsomal preparation. The 45Ca2+ efflux from the inside-out vesicles, which is equivalent to the Ca2+ influx in intact cells, was increased when the free Ca2+ concentration in the medium was decreased. The Ca2+ antagonists La3+ and Co2+ inhibited the 45Ca2+ efflux from the vesicles. Neomycin stimulated the Ca2+ efflux in the presence of either a high or a low free Ca2+ concentration. These results confirm that polyvalent cations regulate Ca2+ fluxes through the plasma membrane.

Citing Articles

Inositol 1,4,5-trisphosphate 3-kinase B promotes Ca mobilization and the inflammatory activity of dendritic cells.

Marongiu L, Mingozzi F, Cigni C, Marzi R, Gioia M, Garre M Sci Signal. 2021; 14(676).

PMID: 33785611 PMC: 8091591. DOI: 10.1126/scisignal.aaz2120.

The inositol 1,4,5-trisphosphate receptor is localized on specialized sub-regions of the endoplasmic reticulum in rat liver.

Lievremont J, Hill A, Hilly M, Mauger J Biochem J. 1994; 300 ( Pt 2):419-27.

PMID: 8002947 PMC: 1138179. DOI: 10.1042/bj3000419.

References

Goodman F, Weiss G, Adams H . Alterations by neomycin of 45Ca movements and contractile responses in vascular smooth muscle. J Pharmacol Exp Ther. 1974; 188(2):472-80. View

Guillemette G, Balla T, Baukal A, Catt K . Characterization of inositol 1,4,5-trisphosphate receptors and calcium mobilization in a hepatic plasma membrane fraction. J Biol Chem. 1988; 263(10):4541-8. View

Evans W . A biochemical dissection of the functional polarity of the plasma membrane of the hepatocyte. Biochim Biophys Acta. 1980; 604(1):27-64. DOI: 10.1016/0005-2736(80)90584-2. View

Lotersztajn S, Hanoune J, Pecker F . A high affinity calcium-stimulated magnesium-dependent ATPase in rat liver plasma membranes. Dependence of an endogenous protein activator distinct from calmodulin. J Biol Chem. 1981; 256(21):11209-15. View

Downes C, Michell R . The polyphosphoinositide phosphodiesterase of erythrocyte membranes. Biochem J. 1981; 198(1):133-40. PMC: 1163219. DOI: 10.1042/bj1980133. View

Lipsky J, Lietman P . Aminoglycoside inhibition of a renal phosphatidylinositol phospholipase C. J Pharmacol Exp Ther. 1982; 220(2):287-92. View

Parker J, Barritt G . Evidence that lanthanum ions stimulate calcium inflow to isolated hepatocytes. Biochem J. 1981; 200(1):109-14. PMC: 1163508. DOI: 10.1042/bj2000109. View

Sastrasinh M, Knauss T, Weinberg J, Humes H . Identification of the aminoglycoside binding site in rat renal brush border membranes. J Pharmacol Exp Ther. 1982; 222(2):350-8. View

Tsien R, Pozzan T, Rink T . Calcium homeostasis in intact lymphocytes: cytoplasmic free calcium monitored with a new, intracellularly trapped fluorescent indicator. J Cell Biol. 1982; 94(2):325-34. PMC: 2112871. DOI: 10.1083/jcb.94.2.325. View

10.

Biber J, Murer H, Carafoli E . Calcium uptake in isolated hepatic plasma-membrane vesicles. Eur J Biochem. 1982; 129(1):7-12. DOI: 10.1111/j.1432-1033.1982.tb07014.x. View

11.

Chan K, Junger K . Calcium transport and phosphorylated intermediate of (Ca2+ + Mg2+)-ATPase in plasma membranes of rat liver. J Biol Chem. 1983; 258(7):4404-10. View

12.

Charest R, Blackmore P, Berthon B, EXTON J . Changes in free cytosolic Ca2+ in hepatocytes following alpha 1-adrenergic stimulation. Studies on Quin-2-loaded hepatocytes. J Biol Chem. 1983; 258(14):8769-73. View

13.

Marche P, Koutouzov S, Girard A . Impairment of membrane phosphoinositide metabolism by aminoglycoside antibiotics: streptomycin, amikacin, kanamycin, dibekacin, gentamicin and neomycin. J Pharmacol Exp Ther. 1983; 227(2):415-20. View

14.

Burgess G, McKinney J, Fabiato A, Leslie B, Putney Jr J . Calcium pools in saponin-permeabilized guinea pig hepatocytes. J Biol Chem. 1983; 258(24):15336-45. View

15.

Prpic V, GREEN K, Blackmore P, EXTON J . Vasopressin-, angiotensin II-, and alpha 1-adrenergic-induced inhibition of Ca2+ transport by rat liver plasma membrane vesicles. J Biol Chem. 1984; 259(3):1382-5. View

16.

Berthon B, Binet A, Mauger J, Claret M . Cytosolic free Ca2+ in isolated rat hepatocytes as measured by quin2. Effects of noradrenaline and vasopressin. FEBS Lett. 1984; 167(1):19-24. DOI: 10.1016/0014-5793(84)80824-8. View

17.

Dawson A, Irvine R . Inositol (1,4,5)trisphosphate-promoted Ca2+ release from microsomal fractions of rat liver. Biochem Biophys Res Commun. 1984; 120(3):858-64. DOI: 10.1016/s0006-291x(84)80186-2. View

18.

Mauger J, POGGIOLI J, Guesdon F, Claret M . Noradrenaline, vasopressin and angiotensin increase Ca2+ influx by opening a common pool of Ca2+ channels in isolated rat liver cells. Biochem J. 1984; 221(1):121-7. PMC: 1144010. DOI: 10.1042/bj2210121. View

19.

Berridge M, Irvine R . Inositol trisphosphate, a novel second messenger in cellular signal transduction. Nature. 1984; 312(5992):315-21. DOI: 10.1038/312315a0. View

20.

Epping R, Bygrave F . A procedure for the rapid isolation from rat liver of plasma membrane vesicles exhibiting Ca2+-transport and Ca2+-ATPase activities. Biochem J. 1984; 223(3):733-45. PMC: 1144357. DOI: 10.1042/bj2230733. View