Antimicrobial Activity of Epsilon-Poly-L-lysine Against Phytopathogenic Bacteria

Overview

Journal Sci Rep

Specialty Science

Date 2020 Jul 11

PMID 32647256

Citations 16

Authors

Barbara Rodrigues

Tamara P Morais

Paulo A Zaini

Cassio S Campos

Hebreia O Almeida-Souza

Abhaya M Dandekar

Rafael Nascimento

Luiz R Goulart

Affiliations

Soon will be listed here.

Abstract

Antimicrobial peptides (AMPs) are components of immune defense in many organisms, including plants. They combat pathogens due to their antiviral, antifungal and antibacterial properties, and are considered potential therapeutic agents. An example of AMP is Epsilon-Poly-L-lysine (EPL), a polypeptide formed by ~ 25 lysine residues with known antimicrobial activity against several human microbial pathogens. EPL presents some advantages such as good water solubility, thermal stability, biodegradability, and low toxicity, being a candidate for the control of phytopathogens. Our aim was to evaluate the antimicrobial activity of EPL against four phytobacterial species spanning different classes within the Gram-negative phylum Proteobacteria: Agrobacterium tumefaciens (syn. Rhizobium radiobacter), Ralstonia solanacearum, Xanthomonas citri subsp. citri (X. citri), and Xanthomonas euvesicatoria. The minimum inhibitory concentration (MIC) of the peptide ranged from 80 μg/ml for X. citri to 600 μg/ml for R. solanacearum and X. euvesicatoria. Two hours of MIC exposure led to pathogen death due to cell lysis and was enough for pathogen clearance. The protective and curative effects of EPL were demonstrated on tomato plants inoculated with X. euvesicatoria. Plants showed less disease severity when sprayed with EPL solution, making it a promising natural product for the control of plant diseases caused by diverse Proteobacteria.

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References

Kering K, Kibii B, Wei H . Biocontrol of phytobacteria with bacteriophage cocktails. Pest Manag Sci. 2019; 75(7):1775-1781. DOI: 10.1002/ps.5324. View

Yuliar , Nion Y, Toyota K . Recent trends in control methods for bacterial wilt diseases caused by Ralstonia solanacearum. Microbes Environ. 2015; 30(1):1-11. PMC: 4356456. DOI: 10.1264/jsme2.ME14144. View

Subramoni S, Nathoo N, Klimov E, Yuan Z . Agrobacterium tumefaciens responses to plant-derived signaling molecules. Front Plant Sci. 2014; 5:322. PMC: 4086400. DOI: 10.3389/fpls.2014.00322. View

Lacroix B, Citovsky V . Transfer of DNA from Bacteria to Eukaryotes. mBio. 2016; 7(4). PMC: 4958254. DOI: 10.1128/mBio.00863-16. View

Potnis N, Timilsina S, Strayer A, Shantharaj D, Barak J, Paret M . Bacterial spot of tomato and pepper: diverse Xanthomonas species with a wide variety of virulence factors posing a worldwide challenge. Mol Plant Pathol. 2015; 16(9):907-20. PMC: 6638463. DOI: 10.1111/mpp.12244. View

Ahmed F, Larrea-Sarmiento A, Alvarez A, Arif M . Genome-informed diagnostics for specific and rapid detection of Pectobacterium species using recombinase polymerase amplification coupled with a lateral flow device. Sci Rep. 2018; 8(1):15972. PMC: 6206099. DOI: 10.1038/s41598-018-34275-0. View

Guell I, Vila S, Badosa E, Montesinos E, Feliu L, Planas M . Design, synthesis, and biological evaluation of cyclic peptidotriazoles derived from BPC194 as novel agents for plant protection. Biopolymers. 2016; 108(3). DOI: 10.1002/bip.23012. View

Morais T, Zaini P, Chakraborty S, Gouran H, Carvalho C, Almeida-Souza H . The plant-based chimeric antimicrobial protein SlP14a-PPC20 protects tomato against bacterial wilt disease caused by Ralstonia solanacearum. Plant Sci. 2019; 280:197-205. DOI: 10.1016/j.plantsci.2018.11.017. View

Jenssen H, Hamill P, Hancock R . Peptide antimicrobial agents. Clin Microbiol Rev. 2006; 19(3):491-511. PMC: 1539102. DOI: 10.1128/CMR.00056-05. View

10.

Shima S, Matsuoka H, Iwamoto T, Sakai H . Antimicrobial action of epsilon-poly-L-lysine. J Antibiot (Tokyo). 1984; 37(11):1449-55. DOI: 10.7164/antibiotics.37.1449. View

11.

Yoshida T, Nagasawa T . epsilon-Poly-L-lysine: microbial production, biodegradation and application potential. Appl Microbiol Biotechnol. 2003; 62(1):21-6. DOI: 10.1007/s00253-003-1312-9. View

12.

Takehara M, Hibino A, Saimura M, Hirohara H . High-yield production of short chain length poly(epsilon-L-lysine) consisting of 5-20 residues by Streptomyces aureofaciens, and its antimicrobial activity. Biotechnol Lett. 2010; 32(9):1299-303. DOI: 10.1007/s10529-010-0294-9. View

13.

Ye R, Xu H, Wan C, Peng S, Wang L, Xu H . Antibacterial activity and mechanism of action of ε-poly-L-lysine. Biochem Biophys Res Commun. 2013; 439(1):148-53. DOI: 10.1016/j.bbrc.2013.08.001. View

14.

Hyldgaard M, Mygind T, Vad B, Stenvang M, Otzen D, Meyer R . The antimicrobial mechanism of action of epsilon-poly-l-lysine. Appl Environ Microbiol. 2014; 80(24):7758-70. PMC: 4249222. DOI: 10.1128/AEM.02204-14. View

15.

Berney M, Hammes F, Bosshard F, Weilenmann H, Egli T . Assessment and interpretation of bacterial viability by using the LIVE/DEAD BacLight Kit in combination with flow cytometry. Appl Environ Microbiol. 2007; 73(10):3283-90. PMC: 1907116. DOI: 10.1128/AEM.02750-06. View

16.

Guo R, McGoverin C, Swift S, Vanholsbeeck F . A rapid and low-cost estimation of bacteria counts in solution using fluorescence spectroscopy. Anal Bioanal Chem. 2017; 409(16):3959-3967. PMC: 5437196. DOI: 10.1007/s00216-017-0347-1. View

17.

Tan Z, Bo T, Guo F, Cui J, Jia S . Effects of ε-Poly-l-lysine on the cell wall of Saccharomyces cerevisiae and its involved antimicrobial mechanism. Int J Biol Macromol. 2018; 118(Pt B):2230-2236. DOI: 10.1016/j.ijbiomac.2018.07.094. View