Proteomic Profiling of Using a Three-Dimensional Separation Method Combined with Tandem Mass Spectrometry

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2020 Jul 8

PMID 32630776

Citations 2

Authors

Goyeun Yun

Jong-Moon Park

Van-An Duong

Jeong-Hun Mok

Jongho Jeon

Onyou Nam

Joonwon Lee

EonSeon Jin

Hookeun Lee

Affiliations

Soon will be listed here.

Abstract

is one of the most abundant marine planktons, and it has a crucial feature in the carbon cycle. However, proteomic analyses of have not been done extensively. In this study, a three-dimensional liquid chromatography (3D-LC) system consisting of strong cation exchange, high- and low-pH reversed-phase liquid chromatography was established for in-depth proteomic profiling of . From tryptic proteome digest, 70 fractions were generated and analyzed using liquid chromatography-tandem mass spectrometry. In total, more than 84,000 unique peptides and 10,000 proteins groups were identified with a false discovery rate of ≤0.01. The physicochemical properties of the identified peptides were evaluated. Using ClueGO, approximately 700 gene ontology terms and 15 pathways were defined from the identified protein groups with -value ≤0.05, covering a wide range of biological processes, cellular components, and molecular functions. Many biological processes associated with CO fixation, photosynthesis, biosynthesis, and metabolic process were identified. Various molecular functions relating to protein binding and enzyme activities were also found. The 3D-LC strategy is a powerful approach for comparative proteomic studies on to reveal changes in its protein level and related mechanism.

Citing Articles

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Duong V, Nam O, Jin E, Park J, Lee H Molecules. 2021; 26(7).

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