Changes in Gene Expression in Potato Meristems Treated with the Sprout Suppressor 1,4-dimethylnaphthalene Are Dependent on Tuber Age and Dormancy Status

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2020 Jul 3

PMID 32614863

Citations 2

Authors

Michael A Campbell

Carley Gwin

Helen H Tai

Rachael Adams

Affiliations

Soon will be listed here.

Abstract

Commercial storage of potatoes often relies on the use of sprout inhibitors to prolong storage and reduce spoilage. The compound 1,4-dimethylnaphthalene (DMN) has seen increase application as a sprout inhibitor in the potato industry as older chemistries are being phased out. The mode of action of DMN is poorly understood as is the sensitivity of potato tissues to this new class of inhibitor. During storage potato tubers transition from a state of endo-dormant to eco-dormant and it is not known if the DMN response is consistent across this developmental transition. RNA-seq gene expression profiling was used to establish if stored potato tubers (Solanum tuberosum cv La Chipper) have differential sensitivity to DMN as tubers age. DMN was applied at three different times during storage; just after harvest when tubers are in endo-dormancy, midwinter at early eco-dormancy, and in spring during late eco-dormancy when sprouting was prevented via exposure to cold storage temperatures. Changes in gene expression were lowest during endo-dormancy while midwinter and spring treatments exhibited a greater and more diverse expression response. Functional analysis of differential gene expression demonstrated gene sets associated with DNA replication, cell division, and DNA methylation are suppressed after DMN treatment. However, gene sets associated with salicylic acid, jasmonic acid, abiotic and biotic stress responses are elevated by DMN only after endodormancy terminates. Gene clusters associated with pathogenesis related proteins PR-4 and PR-5 are also upregulated in response to DMN. These results indicate that DMN sensitivity changes as potato tubers age and transition from endo-dormant to eco-dormant in storage and the overall response is a shift in gene classes that regulate growth and response to stress.

Citing Articles

Physiological and molecular mechanisms associated with potato tuber dormancy.

Dogramaci M, Dobry E, Fortini E, Sarkar D, Eshel D, Campbell M J Exp Bot. 2024; 75(19):6093-6109.

PMID: 38650389 PMC: 11480654. DOI: 10.1093/jxb/erae182.

Evaluating Ecologically Acceptable Sprout Suppressants for Enhancing Dormancy and Potato Storability: A Review.

Gumbo N, Magwaza L, Ngobese N Plants (Basel). 2021; 10(11).

PMID: 34834670 PMC: 8624915. DOI: 10.3390/plants10112307.

References

Seo P, Lee A, Xiang F, Park C . Molecular and functional profiling of Arabidopsis pathogenesis-related genes: insights into their roles in salt response of seed germination. Plant Cell Physiol. 2008; 49(3):334-44. DOI: 10.1093/pcp/pcn011. View

Neilson J, Lague M, Thomson S, Aurousseau F, Murphy A, Bizimungu B . Gene expression profiles predictive of cold-induced sweetening in potato. Funct Integr Genomics. 2017; 17(4):459-476. DOI: 10.1007/s10142-017-0549-9. View

Lawrence S, Novak N, Ju C, Cooke J . Potato, Solanum tuberosum, defense against Colorado potato beetle, Leptinotarsa decemlineata (Say): microarray gene expression profiling of potato by Colorado potato beetle regurgitant treatment of wounded leaves. J Chem Ecol. 2008; 34(8):1013-25. DOI: 10.1007/s10886-008-9507-2. View

Tai H, Lague M, Thomson S, Aurousseau F, Neilson J, Murphy A . Tuber transcriptome profiling of eight potato cultivars with different cold-induced sweetening responses to cold storage. Plant Physiol Biochem. 2019; 146:163-176. DOI: 10.1016/j.plaphy.2019.11.001. View

Stanford A, Northcote D, Bevan M . Spatial and temporal patterns of transcription of a wound-induced gene in potato. EMBO J. 1990; 9(3):593-603. PMC: 551712. DOI: 10.1002/j.1460-2075.1990.tb08151.x. View

Stanford A, Bevan M, Northcote D . Differential expression within a family of novel wound-induced genes in potato. Mol Gen Genet. 1989; 215(2):200-8. DOI: 10.1007/BF00339718. View

Livak K, Schmittgen T . Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2002; 25(4):402-8. DOI: 10.1006/meth.2001.1262. View

Paul V, Ezekiel R, Pandey R . Sprout suppression on potato: need to look beyond CIPC for more effective and safer alternatives. J Food Sci Technol. 2016; 53(1):1-18. PMC: 4711416. DOI: 10.1007/s13197-015-1980-3. View

Irigoyen M, Garceau D, Bohorquez-Chaux A, Becerra Lopez-Lavalle L, Perez-Fons L, Fraser P . Genome-wide analyses of cassava Pathogenesis-related (PR) gene families reveal core transcriptome responses to whitefly infestation, salicylic acid and jasmonic acid. BMC Genomics. 2020; 21(1):93. PMC: 6990599. DOI: 10.1186/s12864-019-6443-1. View

10.

Singh N, Bracker C, Hasegawa P, Handa A, Buckel S, Hermodson M . Characterization of osmotin : a thaumatin-like protein associated with osmotic adaptation in plant cells. Plant Physiol. 1987; 85(2):529-36. PMC: 1054289. DOI: 10.1104/pp.85.2.529. View

11.

Trapnell C, Williams B, Pertea G, Mortazavi A, Kwan G, van Baren M . Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation. Nat Biotechnol. 2010; 28(5):511-5. PMC: 3146043. DOI: 10.1038/nbt.1621. View

12.

Edgar R, Domrachev M, Lash A . Gene Expression Omnibus: NCBI gene expression and hybridization array data repository. Nucleic Acids Res. 2001; 30(1):207-10. PMC: 99122. DOI: 10.1093/nar/30.1.207. View

13.

Campbell M, Gleichsner A, Alsbury R, Horvath D, Suttle J . The sprout inhibitors chlorpropham and 1,4-dimethylnaphthalene elicit different transcriptional profiles and do not suppress growth through a prolongation of the dormant state. Plant Mol Biol. 2010; 73(1-2):181-9. DOI: 10.1007/s11103-010-9607-6. View

14.

Svensson B, Svendsen I, Hojrup P, Roepstorff P, Ludvigsen S, Poulsen F . Primary structure of barwin: a barley seed protein closely related to the C-terminal domain of proteins encoded by wound-induced plant genes. Biochemistry. 1992; 31(37):8767-70. DOI: 10.1021/bi00152a012. View

15.

Kasprzewska A . Plant chitinases--regulation and function. Cell Mol Biol Lett. 2003; 8(3):809-24. View

16.

Bertini L, Proietti S, Aleandri M, Mondello F, Sandini S, Caporale C . Modular structure of HEL protein from Arabidopsis reveals new potential functions for PR-4 proteins. Biol Chem. 2013; 393(12):1533-46. DOI: 10.1515/hsz-2012-0225. View

17.

Campbell M, Gleichsner A, Hilldorfer L, Horvath D, Suttle J . The sprout inhibitor 1,4-dimethylnaphthalene induces the expression of the cell cycle inhibitors KRP1 and KRP2 in potatoes. Funct Integr Genomics. 2011; 12(3):533-41. DOI: 10.1007/s10142-011-0257-9. View

18.

Cheng Y, Liu H, Cao L, Wang S, Li Y, Zhang Y . Down-regulation of multiple CDK inhibitor ICK/KRP genes promotes cell proliferation, callus induction and plant regeneration in Arabidopsis. Front Plant Sci. 2015; 6:825. PMC: 4602110. DOI: 10.3389/fpls.2015.00825. View

19.

Singh A, Jain D, Tyagi C, Singh S, Kumar S, Singh I . In silico prediction of active site and in vitro DNase and RNase activities of Helicoverpa-inducible pathogenesis related-4 protein from Cicer arietinum. Int J Biol Macromol. 2018; 113:869-880. DOI: 10.1016/j.ijbiomac.2018.03.027. View

20.

Devoto A, Ellis C, Magusin A, Chang H, Chilcott C, Zhu T . Expression profiling reveals COI1 to be a key regulator of genes involved in wound- and methyl jasmonate-induced secondary metabolism, defence, and hormone interactions. Plant Mol Biol. 2005; 58(4):497-513. DOI: 10.1007/s11103-005-7306-5. View