Influence of Genetic Diversity of Seventeen Beauveria Bassiana Isolates from Different Hosts on Virulence by Comparative Genomics

Overview

Journal BMC Genomics

Publisher Biomed Central

Specialty Genetics

Date 2020 Jul 2

PMID 32605539

Citations 10

Authors

Zhengkun Zhang

Yang Lu

Wenjing Xu

Li Sui

Qian Du

Yangzhou Wang

Yu Zhao

Qiyun Li

Affiliations

Soon will be listed here.

Abstract

Background: Beauveria bassiana (B. bassiana) is a famous entomopathogenic fungus that could parasitize on hundreds of insect species, which are being used as an environmentally friendly mycoinsecticide. Nevertheless, the possible effect of genetic diversity of these B. bassiana isolates from different hosts on virulence has not been explored before. In order to explore that issue, we compared the genome sequences among seventeen B. bassiana isolates from 17 different insects using whole genome re-sequencing, with B. bassiana strain ARSEF 2860 as the reference genome.

Results: There were a total of 10,098 missense mutated genes, 720 positively selected genes were identified in 17 strains of B. bassiana. Among these, two genes with high frequency mutations encode the toxin-producing non-ribosomal peptide synthase (NRPS) protein. Seven genes undergoing positive selection were enriched in the two-component signaling pathway that is known to regulate the fungal toxicity. In addition, the domain changes of three positively selected genes are also directly related to the virulence plasticity. Besides, the functional categorization of mutated genes showed that most of them involved in the biological functions of toxic proteins involved in.

Conclusions: Based on our data, our results indicate that several mutated genes and positively selected genes may underpin virulence of B. bassiana towards hosts during infection process, which provide an insight into the potential effects of natural variation on the virulence of B. bassiana, which will be useful in screening out potential virulence factors in B. bassiana.

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