Immunomodulatory Effects of a Low-Molecular Weight Polysaccharide from on RAW 264.7 Macrophages and Cyclophosphamide- Induced Immunosuppression Mouse Models

Overview

Journal Mar Drugs

Publisher MDPI

Specialties Biology
Pharmacology

Date 2020 Jul 2

PMID 32605327

Citations 10

Authors

Yingjuan Liu

Xiaolin Wu

Weihua Jin

Yunliang Guo

Affiliations

Soon will be listed here.

Abstract

The water-soluble polysaccharide EP2, from , belongs to the group of polysaccharides known as glucuronoxylorhamnan, which mainly contains glucuronic acid (GlcA), xylose (Xyl), and rhamnose (Rha). The aim of this study was to detect the immunomodulatory effects of EP2 on RAW 264.7 macrophages and cyclophosphamide (CYP)-induced immunosuppression mouse models. The cells were treated with EP2 for different time periods (0, 0.5, 1, 3, and 6 h). The results showed that EP2 promoted nitric oxide production and up-regulated the expression of pro-inflammatory cytokines, such as IL-1β, IL-6, and TNF-α, in a time-dependent manner. Furthermore, we found that EP2-activated iNOS, COX2, and NLRP3 inﬂammasomes, and the TLR4/MAPK/NF-κB signaling pathway played an important role. Moreover, EP2 significantly increased the body weight, spleen index, thymus index, inflammatory cell counts, and the levels of IL-1β, IL-6, and TNF-α in CYP-induced immunosuppression mouse models. These results indicate that EP2 might be a potential immunomodulatory drug and provide the scientific basis for the comprehensive utilization and evaluation of E. prolifera in future applications.

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