Molecular Cloning of a CDNA Encoding the Human Sm-D Autoantigen

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1988 Jul 1

PMID 3260384

Citations 47

Authors

L A Rokeach

J A Haselby

S O Hoch

Affiliations

Soon will be listed here.

Abstract

Antibodies to the Sm-D polypeptide antigen are closely associated with the rheumatic disease systemic lupus erythematosus. Sm-D exists in the cell as one of the core proteins of the small nuclear ribonucleoprotein complexes implicated in RNA processing. We have isolated a cDNA clone, D45-2, coding for the Sm-D human nuclear antigen by screening a human B-lymphocyte cDNA library with synthetic oligonucleotide probes. The 1633-base-pair clone contains an open reading frame (ORF) 357 nucleotides long, capable of encoding a 13,282-dalton polypeptide. The Sm-D coding region is initiated at an AUG codon downstream from a sequence with excellent match to the consensus for the eukaryotic ribosome-binding site. The Sm-D ORF is preceded by a 150-nucleotide-long untranslated leader and followed by a 1126-nucleotide-long untranslated region containing four putative poly(A) signals. The predicted amino acid sequence reveals a (Gly-Arg)9 repeated motif at the C terminus, which may constitute one of the Sm-D immunoreactive determinants. Moreover, this C terminus shows interesting features: (i) a good homology to protamines as expected for a nucleic acid binding protein and (ii) a striking similarity to a region in the Epstein-Barr nuclear antigen.

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References

Earnshaw W, Sullivan K, Machlin P, Cooke C, Kaiser D, Pollard T . Molecular cloning of cDNA for CENP-B, the major human centromere autoantigen. J Cell Biol. 1987; 104(4):817-29. PMC: 2114438. DOI: 10.1083/jcb.104.4.817. View

Bringmann P, Luhrmann R . Purification of the individual snRNPs U1, U2, U5 and U4/U6 from HeLa cells and characterization of their protein constituents. EMBO J. 1986; 5(13):3509-16. PMC: 1167387. DOI: 10.1002/j.1460-2075.1986.tb04676.x. View

Swanson M, Nakagawa T, Levan K, Dreyfuss G . Primary structure of human nuclear ribonucleoprotein particle C proteins: conservation of sequence and domain structures in heterogeneous nuclear RNA, mRNA, and pre-rRNA-binding proteins. Mol Cell Biol. 1987; 7(5):1731-9. PMC: 365274. DOI: 10.1128/mcb.7.5.1731-1739.1987. View

Stanford D, Rohleder A, Neiswanger K, Wieben E . DNA sequence of a human Sm autoimmune antigen. The multigene family contains a processed pseudogene. J Biol Chem. 1987; 262(21):9931-4. View

Oldstone M . Molecular mimicry and autoimmune disease. Cell. 1987; 50(6):819-20. DOI: 10.1016/0092-8674(87)90507-1. View

Spritz R, Strunk K, Surowy C, Hoch S, Barton D, Francke U . The human U1-70K snRNP protein: cDNA cloning, chromosomal localization, expression, alternative splicing and RNA-binding. Nucleic Acids Res. 1987; 15(24):10373-91. PMC: 339950. DOI: 10.1093/nar/15.24.10373. View

Mead D, Kemper B . Single-stranded DNA 'blue' T7 promoter plasmids: a versatile tandem promoter system for cloning and protein engineering. Protein Eng. 1986; 1(1):67-74. DOI: 10.1093/protein/1.1.67. View

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

Chou P, Fasman G . Prediction of the secondary structure of proteins from their amino acid sequence. Adv Enzymol Relat Areas Mol Biol. 1978; 47:45-148. DOI: 10.1002/9780470122921.ch2. View

10.

Towbin H, Staehelin T, Gordon J . Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979; 76(9):4350-4. PMC: 411572. DOI: 10.1073/pnas.76.9.4350. View

11.

Lerner M, Steitz J . Antibodies to small nuclear RNAs complexed with proteins are produced by patients with systemic lupus erythematosus. Proc Natl Acad Sci U S A. 1979; 76(11):5495-9. PMC: 411675. DOI: 10.1073/pnas.76.11.5495. View

12.

White P, Billings P, Hoch S . Assays for the Sm and RNP autoantigens: the requirement for RNA and influence of the tissue source. J Immunol. 1982; 128(6):2751-6. View

13.

Wilbur W, Lipman D . Rapid similarity searches of nucleic acid and protein data banks. Proc Natl Acad Sci U S A. 1983; 80(3):726-30. PMC: 393452. DOI: 10.1073/pnas.80.3.726. View

14.

Liautard J, Brunel C, Jeanteur P . Structural organization of ribonucleoproteins containing small nuclear RNAs from HeLa cells. Proteins interact closely with a similar structural domain of U1, U2, U4 and U5 small nuclear RNAs. J Mol Biol. 1982; 162(3):623-43. DOI: 10.1016/0022-2836(82)90392-8. View

15.

Hunkapiller M, Lujan E, Ostrander F, HOOD L . Isolation of microgram quantities of proteins from polyacrylamide gels for amino acid sequence analysis. Methods Enzymol. 1983; 91:227-36. DOI: 10.1016/s0076-6879(83)91019-4. View

16.

AIKEN J, McKenzie D, Zhao H, States J, DIXON G . Sequence homologies in the protamine gene family of rainbow trout. Nucleic Acids Res. 1983; 11(14):4907-22. PMC: 326094. DOI: 10.1093/nar/11.14.4907. View

17.

Hennessy K, Kieff E . One of two Epstein-Barr virus nuclear antigens contains a glycine-alanine copolymer domain. Proc Natl Acad Sci U S A. 1983; 80(18):5665-9. PMC: 384319. DOI: 10.1073/pnas.80.18.5665. View

18.

Speckert W, Kennedy B, Daisley S, Davies P . Primary structure of protamine from the Northern pike Esox lucius. Eur J Biochem. 1983; 136(2):283-9. DOI: 10.1111/j.1432-1033.1983.tb07739.x. View

19.

Young R, Davis R . Yeast RNA polymerase II genes: isolation with antibody probes. Science. 1983; 222(4625):778-82. DOI: 10.1126/science.6356359. View

20.

Chan Y, Gutell R, Noller H, Wool I . The nucleotide sequence of a rat 18 S ribosomal ribonucleic acid gene and a proposal for the secondary structure of 18 S ribosomal ribonucleic acid. J Biol Chem. 1984; 259(1):224-30. View