Cutaneous Wound Healing: Canine Allogeneic ASC Therapy

Overview

Journal Stem Cell Res Ther

Publisher Biomed Central

Date 2020 Jul 1

PMID 32600465

Citations 17

Authors

Nathaly Enciso

Luis Avedillo

Maria Luisa Fermin

Cristina Fragio

Concepcion Tejero

Affiliations

Soon will be listed here.

Abstract

Background: Wound healing is a complex biological process comprised of a series of sequential events aiming to repair injured tissue. Adult mesenchymal stem cells (MSCs) have been used in cellular therapy in preclinical animal studies; a promising source of MSCs is adipose tissue (AT). In this paper, we evaluated the clinical value and safety of the application of cultured allogenic MSCs from AT for acute and chronic skin wound healing in a canine model.

Methods: Twenty-four dogs of different breeds between 1 and 10 years of age with acute and chronic wounds were studied. Morphology of the wounded skin was monitored for changes over time via serial photographs and histopathological studies.

Results: The percentage of the wounds that exhibited contraction and re-epithelialization were significantly different between wounds treated with adipose mesenchymal stem cells (ASCs) and control wounds; this effect was observed in both acute and chronic conditions. At 90 days, re-epithelization of acute and chronic wounds reached more than 97%. Histopathological study revealed a reduction in inflammatory infiltrate and the presence of multiple hair follicles on day 7 after treatment with ASCs, promoting epidermal and dermal regeneration. To guarantee the safety of our treatment, we determined the serum levels of cytokine markers in our patients. ASC treatment upregulated granulocyte-macrophage colony stimulating factor (GM-CSF) at the gene level, which may contribute to the recruitment of cells that participate in skin repair to the site of injury.

Conclusions: The development of an allogenic ASC therapy to improve wound healing in a canine model could have a clinical impact in human treatment.

Citing Articles

In Situ Treatment of Refractory Perianal Fistulas in Dogs with Low-Dose Allogeneic Adipose-Derived Mesenchymal Stem Cells.

Enciso N, Enciso-Benavides J, Sandoval J, Enciso J Animals (Basel). 2024; 14(22).

PMID: 39595352 PMC: 11591124. DOI: 10.3390/ani14223300.

Therapeutic Application of Extracellular Vesicles Derived from Mesenchymal Stem Cells in Domestic Animals.

Lanci A, Iacono E, Merlo B Animals (Basel). 2024; 14(15).

PMID: 39123673 PMC: 11310970. DOI: 10.3390/ani14152147.

Mesenchymal stem cells in animal reproduction: sources, uses and scenario.

de Oliveira A, Braga A, Miranda J, Fantinato-Neto P, Ambrosio C Braz J Vet Med. 2024; 46:e002524.

PMID: 38737577 PMC: 11087005. DOI: 10.29374/2527-2179.bjvm002524.

Current Advances in Mesenchymal Stem Cell Therapies Applied to Wounds and Skin, Eye, and Neuromuscular Diseases in Companion Animals.

Picazo R, Rojo C, Rodriguez-Quiros J, Gonzalez-Gil A Animals (Basel). 2024; 14(9).

PMID: 38731367 PMC: 11083242. DOI: 10.3390/ani14091363.

Adipose stem cells in tissue regeneration and repair: From bench to bedside.

Dong L, Li X, Leng W, Guo Z, Cai T, Ji X Regen Ther. 2023; 24:547-560.

PMID: 37854632 PMC: 10579872. DOI: 10.1016/j.reth.2023.09.014.

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