LINC01094 Triggers Radio-resistance in Clear Cell Renal Cell Carcinoma Via MiR-577/CHEK2/FOXM1 Axis

Overview

Journal Cancer Cell Int

Publisher Biomed Central

Date 2020 Jun 30

PMID 32595418

Citations 25

Authors

Yufeng Jiang

Wei Li

Yang Yan

Xudong Yao

Wenyu Gu

Haimin Zhang

Affiliations

Soon will be listed here.

Abstract

Background: Radioresistance is an obstacle to limit efficacy of radiotherapy. Meanwhile, long non-coding RNAs (lncRNAs) have been reported to affect radioresistance. Here, we aimed to investigate lncRNAs involving radioresistance development of clear cell renal cell carcinoma (ccRCC), the most frequent type of renal cell carcinoma (RCC).

Methods: The mRNA and protein expressions of genes were measured via qRT-PCR and western blot. The relationships among genes were verified by RIP and luciferase reporter assay. The radioresistance of ccRCC cells was evaluated through clonogenic survival assay, MTT assay and TUNEL assay.

Results: LINC01094 was over-expressed in ccRCC cell lines. LINC01094 expression was increased along with the radiation exposure time and the final stable level was 8 times of the initial level. Knockdown of LINC01094 resulted in enhanced radiosensitivity of ccRCC cells. Mechanically, LINC01094 was a ceRNA of CHEK2 by sponging miR-577. Also, the enhancement of LINC01094 on ccRCC radioresistance was mediated by CHEK2-stabilized FOXM1 protein.

Conclusion: LINC01094 facilitates ccRCC radioresistance by targeting miR-577/CHEK2/FOXM1 axis, blazing a new trail for overcoming radioresistance in ccRCC.

Citing Articles

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LINC01094: A key long non-coding RNA in the regulation of cancer progression and therapeutic targets.

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TGF-β1-Induced LINC01094 promotes epithelial-mesenchymal transition in hepatocellular carcinoma through the miR-122-5p/TGFBR2-SAMD2-SMAD3 Axis.

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References

Schaue D, McBride W . Opportunities and challenges of radiotherapy for treating cancer. Nat Rev Clin Oncol. 2015; 12(9):527-40. PMC: 8396062. DOI: 10.1038/nrclinonc.2015.120. View

Santivasi W, Xia F . Ionizing radiation-induced DNA damage, response, and repair. Antioxid Redox Signal. 2013; 21(2):251-9. DOI: 10.1089/ars.2013.5668. View

Wang Y, Lu Z, Wang N, Feng J, Zhang J, Luan L . Long noncoding RNA DANCR promotes colorectal cancer proliferation and metastasis via miR-577 sponging. Exp Mol Med. 2018; 50(5):1-17. PMC: 5938019. DOI: 10.1038/s12276-018-0082-5. View

Mercer T, Dinger M, Mattick J . Long non-coding RNAs: insights into functions. Nat Rev Genet. 2009; 10(3):155-9. DOI: 10.1038/nrg2521. View

Ficarra V, Guille F, Schips L, de la Taille A, Prayer Galetti T, Tostain J . Proposal for revision of the TNM classification system for renal cell carcinoma. Cancer. 2005; 104(10):2116-23. DOI: 10.1002/cncr.21465. View

Li J, Ji X, Wang H . Targeting Long Noncoding RNA HMMR-AS1 Suppresses and Radiosensitizes Glioblastoma. Neoplasia. 2018; 20(5):456-466. PMC: 5915996. DOI: 10.1016/j.neo.2018.02.010. View

Lichter A, Lawrence T . Recent advances in radiation oncology. N Engl J Med. 1995; 332(6):371-9. DOI: 10.1056/NEJM199502093320607. View

Zannini L, Delia D, Buscemi G . CHK2 kinase in the DNA damage response and beyond. J Mol Cell Biol. 2014; 6(6):442-57. PMC: 4296918. DOI: 10.1093/jmcb/mju045. View

Noh J, Kim K, McClusky W, Abdelmohsen K, Gorospe M . Cytoplasmic functions of long noncoding RNAs. Wiley Interdiscip Rev RNA. 2018; 9(3):e1471. PMC: 5963534. DOI: 10.1002/wrna.1471. View

10.

Kavanagh J, Redmond K, Schettino G, Prise K . DNA double strand break repair: a radiation perspective. Antioxid Redox Signal. 2013; 18(18):2458-72. DOI: 10.1089/ars.2012.5151. View

11.

Lokeshwar S, Talukder A, Yates T, Hennig M, Garcia-Roig M, Lahorewala S . Molecular Characterization of Renal Cell Carcinoma: A Potential Three-MicroRNA Prognostic Signature. Cancer Epidemiol Biomarkers Prev. 2018; 27(4):464-472. DOI: 10.1158/1055-9965.EPI-17-0700. View

12.

Zhang W, Shen C, Li C, Yang G, Liu H, Chen X . miR-577 inhibits glioblastoma tumor growth via the Wnt signaling pathway. Mol Carcinog. 2015; 55(5):575-85. DOI: 10.1002/mc.22304. View

13.

Baumann M, Krause M, Hill R . Exploring the role of cancer stem cells in radioresistance. Nat Rev Cancer. 2008; 8(7):545-54. DOI: 10.1038/nrc2419. View

14.

Zheng R, Yao Q, Ren C, Liu Y, Yang H, Xie G . Upregulation of Long Noncoding RNA Small Nucleolar RNA Host Gene 18 Promotes Radioresistance of Glioma by Repressing Semaphorin 5A. Int J Radiat Oncol Biol Phys. 2016; 96(4):877-887. DOI: 10.1016/j.ijrobp.2016.07.036. View

15.

Tang T, Shan G, Zeng F . Knockdown of DGCR5 enhances the radiosensitivity of human laryngeal carcinoma cells via inducing miR-195. J Cell Physiol. 2018; 234(8):12918-12925. DOI: 10.1002/jcp.27958. View

16.

Chen Y, Li Z, Dong Z, Beebe J, Yang K, Fu L . 14-3-3σ Contributes to Radioresistance By Regulating DNA Repair and Cell Cycle via PARP1 and CHK2. Mol Cancer Res. 2017; 15(4):418-428. PMC: 5380477. DOI: 10.1158/1541-7786.MCR-16-0366. View

17.

Prensner J, Chen W, Iyer M, Cao Q, Ma T, Han S . PCAT-1, a long noncoding RNA, regulates BRCA2 and controls homologous recombination in cancer. Cancer Res. 2014; 74(6):1651-60. PMC: 4009928. DOI: 10.1158/0008-5472.CAN-13-3159. View

18.

Ricketts C, Crooks D, Sourbier C, Schmidt L, Srinivasan R, Linehan W . SnapShot: Renal Cell Carcinoma. Cancer Cell. 2016; 29(4):610-610.e1. DOI: 10.1016/j.ccell.2016.03.021. View

19.

Liu N, Cui W, Jiang X, Zhang Z, Gnosa S, Ali Z . The Critical Role of Dysregulated RhoB Signaling Pathway in Radioresistance of Colorectal Cancer. Int J Radiat Oncol Biol Phys. 2019; 104(5):1153-1164. DOI: 10.1016/j.ijrobp.2019.04.021. View

20.

Lieber M . The mechanism of double-strand DNA break repair by the nonhomologous DNA end-joining pathway. Annu Rev Biochem. 2010; 79:181-211. PMC: 3079308. DOI: 10.1146/annurev.biochem.052308.093131. View