AcGI1, a Novel Genomic Island Carrying Antibiotic Resistance Integron In687 in Multidrug Resistant Achromobacter Xylosoxidans in a Teaching Hospital in Thailand

Overview

Journal FEMS Microbiol Lett

Publisher Oxford University Press

Specialty Microbiology

Date 2020 Jun 28

PMID 32592387

Citations 2

Authors

Pisut Pongchaikul

Pitak Santanirand

Svetlana Antonyuk

Craig Winstanley

Alistair C Darby

Affiliations

Soon will be listed here.

Abstract

This study investigated the genetic basis of multidrug resistance in two strains of Achromobacter xylosoxidans isolated from patients attending a hospital in Thailand in 2012. These isolates were highly resistant to cephalosporins, aminoglycosides, fluoroquinolones, co-trimoxazole and carbapenems. Whole genome sequencing revealed that the two isolates were not clonally related and identified a carbapenem resistance gene-habouring integron (In687), residing in a novel genomic island, AcGI1. This In687 shares 100% identical nucleotide sequence with ones found in Acinetobacter baumannii Aci 16, isolated from the same hospital in 2007. We report the first analysis of multidrug-resistant A. xylosoxidans isolated in Thailand, and the first example of this island in A. xylosoxidans. Our data support the idea that resistance has spread in Thailand via horizontal gene transfer between species and suggest the possibility of A. xylosoxidans may serve as a reservoir of antibiotic resistance, especially in hospital setting.

Citing Articles

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References

Neuwirth C, Freby C, Ogier-Desserrey A, Perez-Martin S, Houzel A, Pechinot A . VEB-1 in Achromobacter xylosoxidans from cystic fibrosis patient, France. Emerg Infect Dis. 2007; 12(11):1737-9. PMC: 3372329. DOI: 10.3201/eid1211.060143. View

Hansen C, Pressler T, Ridderberg W, Johansen H, Skov M . Achromobacter species in cystic fibrosis: cross-infection caused by indirect patient-to-patient contact. J Cyst Fibros. 2013; 12(6):609-15. DOI: 10.1016/j.jcf.2013.05.004. View

Yamamoto M, Nagao M, Hotta G, Matsumura Y, Matsushima A, Ito Y . Molecular characterization of IMP-type metallo-β-lactamases among multidrug-resistant Achromobacter xylosoxidans. J Antimicrob Chemother. 2012; 67(9):2110-3. DOI: 10.1093/jac/dks179. View

Maddamsetti R, Lenski R . Analysis of bacterial genomes from an evolution experiment with horizontal gene transfer shows that recombination can sometimes overwhelm selection. PLoS Genet. 2018; 14(1):e1007199. PMC: 5809092. DOI: 10.1371/journal.pgen.1007199. View

Zhao W, Hu Z . IMP-type metallo-β-lactamases in Gram-negative bacilli: distribution, phylogeny, and association with integrons. Crit Rev Microbiol. 2011; 37(3):214-26. DOI: 10.3109/1040841X.2011.559944. View

Di Pilato V, Pollini S, Rossolini G . Characterization of plasmid pAX22, encoding VIM-1 metallo-β-lactamase, reveals a new putative mechanism of In70 integron mobilization. J Antimicrob Chemother. 2013; 69(1):67-71. DOI: 10.1093/jac/dkt311. View

McArthur A, Waglechner N, Nizam F, Yan A, Azad M, Baylay A . The comprehensive antibiotic resistance database. Antimicrob Agents Chemother. 2013; 57(7):3348-57. PMC: 3697360. DOI: 10.1128/AAC.00419-13. View

Sofianou D, Markogiannakis A, Metzidie E, Pournaras S, Tsakris A . VIM-2 metallo-beta-lactamase in Achromobacter xylosoxidans in Europe. Eur J Clin Microbiol Infect Dis. 2005; 24(12):854-5. DOI: 10.1007/s10096-005-0054-2. View

Jia B, Raphenya A, Alcock B, Waglechner N, Guo P, Tsang K . CARD 2017: expansion and model-centric curation of the comprehensive antibiotic resistance database. Nucleic Acids Res. 2016; 45(D1):D566-D573. PMC: 5210516. DOI: 10.1093/nar/gkw1004. View

10.

Kansakar P, Dorji D, Chongtrakool P, Mingmongkolchai S, Mokmake B, Dubbs P . Local dissemination of multidrug-resistant Acinetobacter baumannii clones in a Thai hospital. Microb Drug Resist. 2010; 17(1):109-19. DOI: 10.1089/mdr.2010.0062. View

11.

Shin K, Han K, Lee J, Hong S, Son B, Youn S . Imipenem-resistant Achromobacter xylosoxidans carrying blaVIM-2-containing class 1 integron. Diagn Microbiol Infect Dis. 2005; 53(3):215-20. DOI: 10.1016/j.diagmicrobio.2005.06.018. View

12.

Sun D, Jeannot K, Xiao Y, Knapp C . Editorial: Horizontal Gene Transfer Mediated Bacterial Antibiotic Resistance. Front Microbiol. 2019; 10:1933. PMC: 6718914. DOI: 10.3389/fmicb.2019.01933. View

13.

Spilker T, Vandamme P, LiPuma J . Identification and distribution of Achromobacter species in cystic fibrosis. J Cyst Fibros. 2012; 12(3):298-301. DOI: 10.1016/j.jcf.2012.10.002. View

14.

Bellissimo F, Pinzone M, Tosto S, Nunnari G, Cacopardo B . Achromobacter xylosoxidans meningitis in an immunosuppressed patient. QJM. 2013; 107(1):65-6. DOI: 10.1093/qjmed/hct170. View

15.

Bador J, Amoureux L, Duez J, Drabowicz A, Siebor E, Llanes C . First description of an RND-type multidrug efflux pump in Achromobacter xylosoxidans, AxyABM. Antimicrob Agents Chemother. 2011; 55(10):4912-4. PMC: 3186983. DOI: 10.1128/AAC.00341-11. View

16.

Braker G, Fesefeldt A, Witzel K . Development of PCR primer systems for amplification of nitrite reductase genes (nirK and nirS) to detect denitrifying bacteria in environmental samples. Appl Environ Microbiol. 1998; 64(10):3769-75. PMC: 106545. DOI: 10.1128/AEM.64.10.3769-3775.1998. View

17.

Bertelli C, Laird M, Williams K, Lau B, Hoad G, Winsor G . IslandViewer 4: expanded prediction of genomic islands for larger-scale datasets. Nucleic Acids Res. 2017; 45(W1):W30-W35. PMC: 5570257. DOI: 10.1093/nar/gkx343. View

18.

Coward A, Kenna D, Perry C, Martin K, Doumith M, Turton J . Use of nrdA gene sequence clustering to estimate the prevalence of different Achromobacter species among Cystic Fibrosis patients in the UK. J Cyst Fibros. 2015; 15(4):479-85. DOI: 10.1016/j.jcf.2015.09.005. View

19.

Stoesser N, Sheppard A, Peirano G, Sebra R, Lynch T, Anson L . First Report of blaIMP-14 on a Plasmid Harboring Multiple Drug Resistance Genes in Escherichia coli Sequence Type 131. Antimicrob Agents Chemother. 2016; 60(8):5068-71. PMC: 4958194. DOI: 10.1128/AAC.00840-16. View

20.

Matsumura Y, Peirano G, Motyl M, Adams M, Chen L, Kreiswirth B . Global Molecular Epidemiology of IMP-Producing Enterobacteriaceae. Antimicrob Agents Chemother. 2017; 61(4). PMC: 5365671. DOI: 10.1128/AAC.02729-16. View