Generating Ventral Spinal Organoids from Human Induced Pluripotent Stem Cells
Overview
Authors
Affiliations
Current advances in human pluripotent stem cell (hPSC) technology allow directed differentiation into three-dimensional spinal organoid cultures that mimic the unique microenvironment and cytoarchitecture of the human spinal cord. Organoids also serves as important cellular tools to model spinal cord development and motor neuron diseases such as Spinal Muscular Atrophy and Amyotrophic Lateral Sclerosis. In this chapter, we describe a detailed step-by-step methodology to generate spinal organoids from human PSCs. We include advantages and limitations of the use of spinal organoids and notes on technical and design considerations in generating these spinal organoids.
Chen X, Liu C, McDaniel G, Zeng O, Ali J, Zhou Y Adv Healthc Mater. 2024; 13(32):e2402199.
PMID: 39300854 PMC: 11671291. DOI: 10.1002/adhm.202402199.
Spinal Cord Organoids to Study Motor Neuron Development and Disease.
Buchner F, Dokuzluoglu Z, Grass T, Rodriguez-Muela N Life (Basel). 2023; 13(6).
PMID: 37374039 PMC: 10303776. DOI: 10.3390/life13061254.
Spinal cord tissue engineering using human primary neural progenitor cells and astrocytes.
Jin C, Wu Y, Zhang H, Xu B, Liu W, Ji C Bioeng Transl Med. 2023; 8(2):e10448.
PMID: 36925694 PMC: 10013752. DOI: 10.1002/btm2.10448.
An Optimized Workflow to Generate and Characterize iPSC-Derived Motor Neuron (MN) Spheroids.
Castellanos-Montiel M, Chaineau M, Franco-Flores A, Haghi G, Carrillo-Valenzuela D, Reintsch W Cells. 2023; 12(4).
PMID: 36831212 PMC: 9954647. DOI: 10.3390/cells12040545.
Progress and challenges in directing the differentiation of human iPSCs into spinal motor neurons.
Castillo Bautista C, Sterneckert J Front Cell Dev Biol. 2023; 10:1089970.
PMID: 36684437 PMC: 9849822. DOI: 10.3389/fcell.2022.1089970.