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Multicentre Comparison of Quantitative PCR-based Assays to Detect SARS-CoV-2, Germany, March 2020

Overview
Journal Euro Surveill
Specialty Infectious Diseases
Date 2020 Jun 26
PMID 32583765
Citations 40
Authors
Maximilian Muenchhoff
Helga Mairhofer
Hans Nitschko
Natascha Grzimek-Koschewa
Dieter Hoffmann
Annemarie Berger
Holger Rabenau
Marek Widera
Nikolaus Ackermann
Regina Konrad
Sabine Zange
Alexander Graf
Stefan Krebs
Helmut Blum
Andreas Sing
Bernhard Liebl
Roman Wolfel
Sandra Ciesek
Christian Drosten
Ulrike Protzer
Stephan Boehm
Oliver T Keppler
Affiliations
Soon will be listed here.
Abstract

Containment strategies and clinical management of coronavirus disease (COVID-19) patients during the current pandemic depend on reliable diagnostic PCR assays for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, we compare 11 different RT-PCR test systems used in seven diagnostic laboratories in Germany in March 2020. While most assays performed well, we identified detection problems in a commonly used assay that may have resulted in false-negative test results during the first weeks of the pandemic.

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