S-methyl Methanethiosulfonate: Promising Late Blight Inhibitor or Broad Range Toxin?

Overview

Journal Pathogens

Date 2020 Jun 26

PMID 32580401

Citations 6

Authors

Charlotte Joller

Mout De Vrieze

Aboubakr Moradi

Claudine Fournier

Delphine Chinchilla

Floriane LHaridon

Sebastien Bruisson

Laure Weisskopf

Affiliations

Soon will be listed here.

Abstract

(1) Background: S-methyl methanethiosulfonate (MMTS), a sulfur containing volatile organic compound produced by plants and bacterial species, has recently been described to be an efficient anti-oomycete agent with promising perspectives for the control of the devastating potato late blight disease caused by However, earlier work raised questions regarding the putative toxicity of this compound. To assess the suitability of MMTS for late blight control in the field, the present study thus aimed at evaluating the effect of MMTS on a wide range of non-target organisms in comparison to . (2) Methods: To this end, we exposed as well as different pathogenic and non-pathogenic fungi, bacteria, the nematode as well as the plant to MMTS treatment and evaluated their response by means of in vitro assays. (3) Results: Our results showed that fungi (both mycelium and spores) tolerated MMTS better than the oomycete but that the compound nevertheless exhibited non-negligible toxic effects on bacteria, nematodes and plants. (4) Conclusions: We discuss the mode of action of MMTS and conclude that even though this compound might be too toxic for chemical application in the field, its strong anti-oomycete activity could still be exploited when naturally released at the site of infection by plant-associated microbes inoculated as biocontrol agents.

Citing Articles

The Volatile Organic Compounds of spp.: An In-Depth Analysis of Their Antifungal Properties.

Cuervo L, Alvarez-Garcia S, Salas J, Mendez C, Olano C, Malmierca M Microorganisms. 2023; 11(7).

PMID: 37512992 PMC: 10384482. DOI: 10.3390/microorganisms11071820.

An agroecological structure model of compost-soil-plant interactions for sustainable organic farming.

Miyamoto H, Shigeta K, Suda W, Ichihashi Y, Nihei N, Matsuura M ISME Commun. 2023; 3(1):28.

PMID: 37002405 PMC: 10066230. DOI: 10.1038/s43705-023-00233-9.

Antibacterial Activity of Endophytic Bacteria Against Sugar Beet Root Rot Agent by Volatile Organic Compound Production and Induction of Systemic Resistance.

Safara S, Harighi B, Bahramnejad B, Ahmadi S Front Microbiol. 2022; 13:921762.

PMID: 35722285 PMC: 9201493. DOI: 10.3389/fmicb.2022.921762.

Phytochemical Profiling of L. Aqueous Extract with Antioxidant, Antimicrobial, Antibiofilm, and Anti-Quorum Sensing Properties: In Vitro and In Silico Studies.

Snoussi M, Noumi E, Hajlaoui H, Bouslama L, Hamdi A, Saeed M Plants (Basel). 2022; 11(4).

PMID: 35214828 PMC: 8878528. DOI: 10.3390/plants11040495.

In vitro anticandidal activity and gas chromatography-mass spectrometry (GC-MS) screening of leaf extracts.

Ababutain I, Alghamdi A PeerJ. 2021; 9:e10561.

PMID: 33505793 PMC: 7789864. DOI: 10.7717/peerj.10561.

References

Fry W, Birch P, Judelson H, Grunwald N, Danies G, Everts K . Five Reasons to Consider Phytophthora infestans a Reemerging Pathogen. Phytopathology. 2015; 105(7):966-81. DOI: 10.1094/PHYTO-01-15-0005-FI. View

De Vrieze M, Pandey P, Bucheli T, Varadarajan A, Ahrens C, Weisskopf L . Volatile Organic Compounds from Native Potato-associated Pseudomonas as Potential Anti-oomycete Agents. Front Microbiol. 2015; 6:1295. PMC: 4655239. DOI: 10.3389/fmicb.2015.01295. View

Rajini P, Melstrom P, Williams P . A comparative study on the relationship between various toxicological endpoints in Caenorhabditis elegans exposed to organophosphorus insecticides. J Toxicol Environ Health A. 2008; 71(15):1043-50. DOI: 10.1080/15287390801989002. View

Hopff W, RIGGIO G, Waser P . Blockade of acetylcholine synthesis in organophosphate poisoning. Toxicol Appl Pharmacol. 1984; 72(3):513-8. DOI: 10.1016/0041-008x(84)90128-5. View

Marks H, ANDERSON J, Stoewsand G . Effect of S-methyl cysteine sulphoxide and its metabolite methyl methane thiosulphinate, both occurring naturally in Brassica vegetables, on mouse genotoxicity. Food Chem Toxicol. 1993; 31(7):491-5. DOI: 10.1016/0278-6915(93)90108-b. View

Derman A, Prinz W, Belin D, Beckwith J . Mutations that allow disulfide bond formation in the cytoplasm of Escherichia coli. Science. 1993; 262(5140):1744-7. DOI: 10.1126/science.8259521. View

Defonsi Lestard M, Diaz S, Tuttolomondo M, Sanchez Cortez S, Puiatti M, Pierini A . Interaction of S-methyl methanethiosulfonate with DPPC bilayer. Spectrochim Acta A Mol Biomol Spectrosc. 2012; 97:479-89. DOI: 10.1016/j.saa.2012.06.045. View

Syed Ab Rahman S, Singh E, Pieterse C, Schenk P . Emerging microbial biocontrol strategies for plant pathogens. Plant Sci. 2018; 267:102-111. DOI: 10.1016/j.plantsci.2017.11.012. View

Kim D, Chun S, Jeon J, Lee S, Joe G . Synthesis and fungicidal activity of ethaboxam against Oomycetes. Pest Manag Sci. 2004; 60(10):1007-12. DOI: 10.1002/ps.873. View

10.

Borlinghaus J, Albrecht F, Gruhlke M, Nwachukwu I, Slusarenko A . Allicin: chemistry and biological properties. Molecules. 2014; 19(8):12591-618. PMC: 6271412. DOI: 10.3390/molecules190812591. View

11.

Haas B, Kamoun S, Zody M, Jiang R, Handsaker R, Cano L . Genome sequence and analysis of the Irish potato famine pathogen Phytophthora infestans. Nature. 2009; 461(7262):393-8. DOI: 10.1038/nature08358. View

12.

Gelhaye E, Rouhier N, Navrot N, Jacquot J . The plant thioredoxin system. Cell Mol Life Sci. 2004; 62(1):24-35. DOI: 10.1007/s00018-004-4296-4. View

13.

Chinchilla D, Bruisson S, Meyer S, Zuhlke D, Hirschfeld C, Joller C . A sulfur-containing volatile emitted by potato-associated bacteria confers protection against late blight through direct anti-oomycete activity. Sci Rep. 2020; 9(1):18778. PMC: 6937334. DOI: 10.1038/s41598-019-55218-3. View

14.

Kamoun S . A catalogue of the effector secretome of plant pathogenic oomycetes. Annu Rev Phytopathol. 2006; 44:41-60. DOI: 10.1146/annurev.phyto.44.070505.143436. View

15.

Neher D . Nematode communities in organically and conventionally managed agricultural soils. J Nematol. 2009; 31(2):142-54. PMC: 2620368. View

16.

OSullivan D, OGara F . Traits of fluorescent Pseudomonas spp. involved in suppression of plant root pathogens. Microbiol Rev. 1992; 56(4):662-76. PMC: 372893. DOI: 10.1128/mr.56.4.662-676.1992. View

17.

Stoewsand G . Bioactive organosulfur phytochemicals in Brassica oleracea vegetables--a review. Food Chem Toxicol. 1995; 33(6):537-43. DOI: 10.1016/0278-6915(95)00017-v. View

18.

Karala A, Ruddock L . Does s-methyl methanethiosulfonate trap the thiol-disulfide state of proteins?. Antioxid Redox Signal. 2007; 9(4):527-31. DOI: 10.1089/ars.2006.1473. View

19.

Hunt P . The C. elegans model in toxicity testing. J Appl Toxicol. 2016; 37(1):50-59. PMC: 5132335. DOI: 10.1002/jat.3357. View

20.

Dorange J, Aranda G, Cornu A, Dulieu H . Genetic toxicity of methyl methanethiosulfonate on Salmonella typhimurium, Saccharomyces cerevisiae and Nicotiana tabacum. Mutat Res. 1983; 120(4):207-17. DOI: 10.1016/0165-7992(83)90092-1. View