Effect of Bovine Leukemia Virus (BLV) Infection on Bovine Mammary Epithelial Cells RNA-seq Transcriptome Profile

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2020 Jun 25

PMID 32579585

Citations 5

Authors

Lucia Martinez Cuesta

Juan Pedro Liron

Maria Victoria Nieto Farias

Guillermina Laura Dolcini

Maria Carolina Ceriani

Affiliations

Soon will be listed here.

Abstract

Bovine leukemia virus (BLV) is a δ-retrovirus responsible for Enzootic Bovine Leukosis (EBL), a lymphoproliferative disease that affects cattle. The virus causes immune system deregulation, favoring the development of secondary infections. In that context, mastitis incidence is believed to be increased in BLV infected cattle. The aim of this study was to analyze the transcriptome profile of a BLV infected mammary epithelial cell line (MAC-T). Our results show that BLV infected MAC-T cells have an altered expression of IFN I signal pathway and genes involved in defense response to virus, as well as a collagen catabolic process and some protooncogenes and tumor suppressor genes. Our results provide evidence to better understand the effect of BLV on bovine mammary epithelial cell's immune response.

Citing Articles

Seroprevalence of bovine leukemia virus and association with bovine infectious abortion in Creole breeds from tropical grazing herds in the Colombian Caribbean.

Oviedo-Pastrana M, Doria-Ramos M, Mattar S, Oviedo-Socarras T, Vallejo-Timaran D Vet World. 2024; 17(8):1715-1721.

PMID: 39328435 PMC: 11422635. DOI: 10.14202/vetworld.2024.1715-1721.

Genetic factors influencing milk and fat yields in tropically adapted dairy cattle: insights from quantitative trait loci analysis and gene associations.

Laodim T, Koonawootrittriron S, Elzo M, Suwanasopee T, Jattawa D, Sarakul M Anim Biosci. 2023; 37(4):576-590.

PMID: 37946425 PMC: 10915225. DOI: 10.5713/ab.23.0246.

Bovine mastitis, a worldwide impact disease: Prevalence, antimicrobial resistance, and viable alternative approaches.

Morales-Ubaldo A, Rivero-Perez N, Valladares-Carranza B, Velazquez-Ordonez V, Delgadillo-Ruiz L, Zaragoza-Bastida A Vet Anim Sci. 2023; 21:100306.

PMID: 37547227 PMC: 10400929. DOI: 10.1016/j.vas.2023.100306.

Milk Macrophage Function in Bovine Leukemia Virus-Infected Dairy Cows.

de Souza Lima E, Blagitz M, Batista C, Alves A, de Carvalho Fernandes A, Ramos Sanchez E Front Vet Sci. 2021; 8:650021.

PMID: 34222393 PMC: 8245700. DOI: 10.3389/fvets.2021.650021.

Correction: Effect of bovine leukemia virus (BLV) infection on bovine mammary epithelial cells RNA-seq transcriptome profile.

PLoS One. 2020; 15(7):e0236912.

PMID: 32702042 PMC: 7377480. DOI: 10.1371/journal.pone.0236912.

References

Lawrence M, Huber W, Pages H, Aboyoun P, Carlson M, Gentleman R . Software for computing and annotating genomic ranges. PLoS Comput Biol. 2013; 9(8):e1003118. PMC: 3738458. DOI: 10.1371/journal.pcbi.1003118. View

Kumar M, Belcaid M, Nerurkar V . Identification of host genes leading to West Nile virus encephalitis in mice brain using RNA-seq analysis. Sci Rep. 2016; 6:26350. PMC: 4876452. DOI: 10.1038/srep26350. View

Howell C, Smith J, Shute J . Targeting matrix metalloproteinase-13 in bronchial epithelial repair. Clin Exp Allergy. 2018; 48(9):1214-1221. DOI: 10.1111/cea.13215. View

Ioannidis I, McNally B, Willette M, Peeples M, Chaussabel D, Durbin J . Plasticity and virus specificity of the airway epithelial cell immune response during respiratory virus infection. J Virol. 2012; 86(10):5422-36. PMC: 3347264. DOI: 10.1128/JVI.06757-11. View

DiMartino J, Lacayo N, Varadi M, Li L, Saraiya C, Ravindranath Y . Low or absent SPARC expression in acute myeloid leukemia with MLL rearrangements is associated with sensitivity to growth inhibition by exogenous SPARC protein. Leukemia. 2006; 20(3):426-32. DOI: 10.1038/sj.leu.2404102. View

Wu X, Wang L, Xue J, Dai Y, Yang D, Qu M . A study of the impact of inhibitors of DNA binding-1 on proliferation and migration in human colon carcinoma cells. Kaohsiung J Med Sci. 2019; 35(4):209-213. DOI: 10.1002/kjm2.12037. View

Dubail J, Kesteloot F, Deroanne C, Motte P, Lambert V, Rakic J . ADAMTS-2 functions as anti-angiogenic and anti-tumoral molecule independently of its catalytic activity. Cell Mol Life Sci. 2010; 67(24):4213-32. PMC: 11115784. DOI: 10.1007/s00018-010-0431-6. View

Huynh H, Robitaille G, Turner J . Establishment of bovine mammary epithelial cells (MAC-T): an in vitro model for bovine lactation. Exp Cell Res. 1991; 197(2):191-9. DOI: 10.1016/0014-4827(91)90422-q. View

Penzo M, Montanaro L, Trere D, Derenzini M . The Ribosome Biogenesis-Cancer Connection. Cells. 2019; 8(1). PMC: 6356843. DOI: 10.3390/cells8010055. View

10.

Parker D, Planet P, Soong G, Narechania A, Prince A . Induction of type I interferon signaling determines the relative pathogenicity of Staphylococcus aureus strains. PLoS Pathog. 2014; 10(2):e1003951. PMC: 3930619. DOI: 10.1371/journal.ppat.1003951. View

11.

Lam W, Yeung A, Chu I, Chan P . Profiles of cytokine and chemokine gene expression in human pulmonary epithelial cells induced by human and avian influenza viruses. Virol J. 2010; 7:344. PMC: 3002310. DOI: 10.1186/1743-422X-7-344. View

12.

Love M, Huber W, Anders S . Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol. 2014; 15(12):550. PMC: 4302049. DOI: 10.1186/s13059-014-0550-8. View

13.

Martinez Cuesta L, Nieto Farias M, Lendez P, Rowland R, Sheahan M, Cheuquepan Valenzuela F . Effect of bovine leukemia virus on bovine mammary epithelial cells. Virus Res. 2019; 271:197678. DOI: 10.1016/j.virusres.2019.197678. View

14.

Katsounas A, Schlaak J, Lempicki R . CCL5: a double-edged sword in host defense against the hepatitis C virus. Int Rev Immunol. 2011; 30(5-6):366-78. DOI: 10.3109/08830185.2011.593105. View

15.

Said N, Frierson H, Sanchez-Carbayo M, Brekken R, Theodorescu D . Loss of SPARC in bladder cancer enhances carcinogenesis and progression. J Clin Invest. 2013; 123(2):751-66. PMC: 3561835. DOI: 10.1172/JCI64782. View

16.

Guo L, Xu X, Zhou L, Zhou R, Wang X, Li J . Human Intestinal Epithelial Cells Release Antiviral Factors That Inhibit HIV Infection of Macrophages. Front Immunol. 2018; 9:247. PMC: 5825896. DOI: 10.3389/fimmu.2018.00247. View

17.

OConnell R, Saha S, Vaidya S, Bruhn K, Miranda G, Zarnegar B . Type I interferon production enhances susceptibility to Listeria monocytogenes infection. J Exp Med. 2004; 200(4):437-45. PMC: 2211937. DOI: 10.1084/jem.20040712. View

18.

Bohnekamp J, Schoneberg T . Cell adhesion receptor GPR133 couples to Gs protein. J Biol Chem. 2011; 286(49):41912-41916. PMC: 3234928. DOI: 10.1074/jbc.C111.265934. View

19.

Carrero J, Calderon B, Unanue E . Type I interferon sensitizes lymphocytes to apoptosis and reduces resistance to Listeria infection. J Exp Med. 2004; 200(4):535-40. PMC: 2211931. DOI: 10.1084/jem.20040769. View

20.

Mantovani A, Allavena P, Sica A, Balkwill F . Cancer-related inflammation. Nature. 2008; 454(7203):436-44. DOI: 10.1038/nature07205. View